We narrowed to 8,877 results for: BLI;
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Plasmid#221079PurposeSEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertTTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
iEscSnFR
Plasmid#182807PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiEscSnFR
UseTags6xHis, HA and MycExpressionBacterialMutationPromoterT7Available sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
iFluoxSnFR
Plasmid#182808PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiFluoxSnFR
UseTags6xHis, HA and MycExpressionBacterialMutationPromoterT7Available sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xQUAS-EBFP2-GMRWhite
Plasmid#165921PurposeBlasticidin resistant 5xQUAS EBFP2 GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable QUAS Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xQUAS-sfGFP-MiniWhite
Plasmid#165922PurposeBlasticidin resistant 5xQUAS sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable QUAS Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xQUAS-EBFP2-MiniWhite
Plasmid#165924PurposeBlasticidin resistant 5xQUAS EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable QUAS Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xUAS-EBFP2-MiniWhite
Plasmid#165912PurposeBlasticidin resistant 5xUAS EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable UAS Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xUAS-mCherry-GMRWhite
Plasmid#165908PurposeBlasticidin resistant 5xUAS mCherry GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable UAS Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xQUAS-mCherry-GMRWhite
Plasmid#165920PurposeBlasticidin resistant 5xQUAS mCherry GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable QUAS Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-12xLexAOp-sfGFP-MiniWhite
Plasmid#165916PurposeBlasticidin resistant 12xLexAOp sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable LexAOp Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xQUAS-mCherry-MiniWhite
Plasmid#165923PurposeBlasticidin resistant 5xQUAS mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable QUAS Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
iSKetSnFR2
Plasmid#140467PurposeDevelop a family of genetically encoded fluorescent biosensors for S-ketamine, termed iSKetSnFR2, thus enabling optical subcellular pharmacokinetics for S-Ketamine.DepositorInsertiSKetSnFR2
UseTagsHis Tag, HA Tag and Myc tagExpressionBacterialMutationPromoterT7Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
iSKetSnFR1
Plasmid#140466PurposeDevelop a family of genetically encoded fluorescent biosensors for S-ketamine, termed iSKetSnFR1, thus enabling optical subcellular pharmacokinetics for S-Ketamine.DepositorInsertiSKetSnFR1
UseTagsHis Tag, HA Tag and Myc tagExpressionBacterialMutationPromoterT7Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
KN501: pMVP (L3-L2) ecDHFR DD + WPRE
Plasmid#121793PurposepMVP L3-L2 entry plasmid, contains ecDHFR DD + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term ecDHFR degron domain (stablized by TMP) to gene in lentivirus vectorsDepositorInsertecDHFR Degron Domain + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFAB1907
Plasmid#47857PurposeBIOFAB reporter plasmid for measuring BBa_B1006 U10 termination efficiencyDepositorInsertBBa_B1006 U10
UseSynthetic BiologyTagsExpressionBacterialMutationAdded two extra U at the end of Bba_1006 poly-U t…PromoterpLTetO12Available sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3989
Plasmid#47820PurposeBIOFAB RFP reporter plasmid for measuring promoter 14 + BCD13 efficiency.DepositorInsertpromoter 14 and BCD13
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3991
Plasmid#47822PurposeBIOFAB RFP reporter plasmid for measuring promoter 14 + BCD15 efficiency.DepositorInsertpromoter 14 and BCD15
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3993
Plasmid#47824PurposeBIOFAB RFP reporter plasmid for measuring promoter 14 + BCD17 efficiency.DepositorInsertpromoter 14 and BCD17
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3994
Plasmid#47825PurposeBIOFAB RFP reporter plasmid for measuring promoter 14 + BCD18 efficiency.DepositorInsertpromoter 14 and BCD18
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3995
Plasmid#47826PurposeBIOFAB RFP reporter plasmid for measuring promoter 14 + BCD19 efficiency.DepositorInsertpromoter 14 and BCD19
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3996
Plasmid#47827PurposeBIOFAB RFP reporter plasmid for measuring promoter 14 + BCD20 efficiency.DepositorInsertpromoter 14 and BCD20
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3998
Plasmid#47829PurposeBIOFAB RFP reporter plasmid for measuring promoter 14 + BCD22 efficiency.DepositorInsertpromoter 14 and BCD22
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3999
Plasmid#47830PurposeBIOFAB RFP reporter plasmid for measuring promoter 14 + BCD23 efficiency.DepositorInsertpromoter 14 and BCD23
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB4000
Plasmid#47831PurposeBIOFAB RFP reporter plasmid for measuring promoter 14 + BCD24 efficiency.DepositorInsertpromoter 14 and BCD24
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3713
Plasmid#47836PurposeBIOFAB RFP reporter plasmid for measuring promoter 5 + BCD1 efficiency.DepositorInsertpromoter 5 and BCD1
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3910
Plasmid#47813PurposeBIOFAB RFP reporter plasmid for measuring promoter 14 + BCD6 efficiency.DepositorInsertpromoter 14 and BCD6
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3914
Plasmid#47817PurposeBIOFAB RFP reporter plasmid for measuring promoter 14 + BCD10 efficiency.DepositorInsertpromoter 14 and BCD10
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3915
Plasmid#47818PurposeBIOFAB RFP reporter plasmid for measuring promoter 14 + BCD11 efficiency.DepositorInsertpromoter 14 and BCD11
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3916
Plasmid#47819PurposeBIOFAB RFP reporter plasmid for measuring promoter 14 + BCD12 efficiency.DepositorInsertpromoter 14 and BCD12
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3911
Plasmid#47814PurposeBIOFAB RFP reporter plasmid for measuring promoter 14 + BCD7 efficiency.DepositorInsertpromoter 14 and BCD7
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3909
Plasmid#47812PurposeBIOFAB RFP reporter plasmid for measuring promoter 14 + BCD5 efficiency.DepositorInsertpromoter 14 and BCD5
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB801
Plasmid#47846PurposeBIOFAB reporter plasmid for measuring trp_att_L126 termination efficiencyDepositorInserttrp_att_L126 terminator
UseSynthetic BiologyTagsExpressionBacterialMutationLoop mutation in trp TerminatorPromoterpLTetO1Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3881
Plasmid#47843PurposeBIOFAB RFP reporter plasmid for measuring promoter 12 + BCD1 efficiency.DepositorInsertpromoter 12 and BCD1
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Frmd4b
Plasmid#45867DepositorInsertFrmd4b in situ probe (Frmd4b Mouse)
UseIn situTagsExpressionMutationcontains bp# 3933-4535 of NM_145148.2PromoterAvailable sinceJuly 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TLR targeting vector
Plasmid#64215PurposeTargeting vector for the human AAVS1 locus for insertion of traffic light reporter (TLR) constructDepositorInserttraffic light reporter (TLR) (AAVS1 Human)
UseHuman targetingTagsExpressionMutationsee publication for detailsPromoterCAGAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-CBE-Cas9n-Act3.0
Plasmid#178955PurposeIt consists of a Cas9 nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.DepositorInserthA3A-zCas9-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMinDis.X513-iNicSnFR3a-(CC93)-PM
Plasmid#125122PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotineDepositorInsertiNicSnFR3a-(CC93)-PM
UseTagsIg-K-Chain and membrane anchoring sequence-PDGFRExpressionMammalianMutationNAPromoterT7Available sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMinDis.X513-iNicSnFR3a-(CC93)-ER
Plasmid#125121PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotineDepositorInsertiNicSnFR3a-(CC93)-ER
UseTagsER retention motifExpressionMammalianMutationPromoterT7Available sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPBT/EFS-Cas9-PuroTk
Plasmid#170542PurposeExpresses spCas9 with a P2A junction to the puromycin resistance gene directly fused to the thymidine kinaseDepositorInsertPuroTK
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-ABE-Cas9n-Act3.0
Plasmid#178956PurposeIt consists of a Cas9 nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.DepositorInsertABE8e-zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGRG36-Kn-PA1-GFP
Plasmid#79088PurposepGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)DepositorInsertGFP
UseBacterial chromosome integration and expression o…TagsExpressionBacterialMutationPromoterPA1Available sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
Human a4 nicotinic subunit crystallization construct
Plasmid#85841PurposepEZT-BM vector with alpha4 gene after CMV promoter, published crystallization constructDepositorInsertHuman a4 nAChR subunit crystallization construct (CHRNA4 Human)
UseBaculovirus (bacmam) production and mammalian exp…TagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-IGK-dAPEX2-KDEL
Plasmid#117183PurposePlasmid name in publication: pAAV-DIO-ER-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertIGK-dAPEX2-KDEL
UseAAV and Cre/LoxTagsExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5 FLAG human DEPTOR
Plasmid#21334DepositorInsertDEPTOR (DEPTOR Human)
UseTagsFLAGExpressionMammalianMutationN204S and S389N mutations (numbering according to…PromoterAvailable sinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
TFORF2031
Plasmid#142906PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPRDM1 (PRDM1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Human b2 nicotinic subunit crystallization construct
Plasmid#85842PurposepEZT-BM vector with beta2 gene after CMV promoter, published crystallization constructDepositorInsertHuman b2 nAChR subunit crystallization construct (CHRNB2 Human)
UseBaculovirus (bacmam) production and mammalian exp…TagsC-terminal StrepII tagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-CBE-SpRYn-Act3.0
Plasmid#178958PurposeIt consists of a SpRY nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2 -SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.DepositorInserthA3A-SpRY-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-ABE-SpRYn-Act3.0
Plasmid#178959PurposeIt consists of a SpRY nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.DepositorInsertABE8e-SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF3486
Plasmid#144962PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPRDM1 (PRDM1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Transposon-for RMCE_Sleeping Beauty_LIR-CAG-Frt-red(katushka)-P2A-Puro-F3-PA-RIR
Plasmid#67275PurposeFor sleeping beauty transposition and establishment of an recombinase mediated cassette exchange docking site. To make RMCE in cell line instead of flp in cell linesDepositorInsertpuromycin resistant gene
UseTagslinked to red flouresent protein gene (katushka)ExpressionMammalianMutationPromoterCAGAvailable sinceJuly 15, 2015AvailabilityAcademic Institutions and Nonprofits only