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pET28a SpyCatcher-SnoopCatcher

Plasmid

#72324

Purpose

Bacterial expression of SpyCatcher linked to SnoopCatcher.

Depositor

Mark Howarth

Publication

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

pET28a SpyCatcher-SnoopCatcher

Tags

N-terminal His6 tag

Expression

Bacterial

Mutation

35 amino acids at the N-term of SpyCatcher have b…

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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scFLARE2

Plasmid

#158700

Purpose

Neuronal expression of scFLARE2 component

Depositor

Alice Ting

Publication

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

scFLARE2

Use

AAV

Tags

HA, Flag, VP16

Expression

Mammalian

Mutation

Truncated form of TEV protease (220-242 amino aci…

Promoter

Syn

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-nEF-ChRmine-mScarlet

Plasmid

#137158

Purpose

Viral expression of ChRmine-p2a-mScarlet

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Publication

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has service

AAV8

Insert

ChRmine-p2a-mScarlet

Use

AAV

Mutation

n/a

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pET28 MBP_PrA/G-H6

Plasmid

#176077

Purpose

Express His6 tagged MBP_PrA/G in Escherichia coli

Depositor

Tom Rapoport

Publication

Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

MBP PrA/G-H6

Tags

His tag

Expression

Bacterial

Promoter

T7 Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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mPlum-pBAD

Plasmid

#54564

Purpose

Localization: Protein Expression Vector , Excitation: 590, Emission: 649

Depositor

Michael Davidson, Roger Tsien

Publication

Wang et al Proc Natl Acad Sci U S A. 2004 Nov 30;10

Type

Empty backbone

Tags

6xHis and mPlum

Expression

Bacterial

Available since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCMV5 HA-TBRII (delta Cyt)

Plasmid

#14051

Depositor

Joan Massague

Publication

Siegel et al Proc Natl Acad Sci U S A. 2003 Jul 8. 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TGF beta rec II (delta cyt) (TGFBR2 Human)

Tags

HA (PYDVPDYA)

Expression

Mammalian

Mutation

Dominant negative mutant. A stop codon was introd…

Available since

May 10, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pUD628

Plasmid

#103018

Purpose

expression of a Cpf1 programming crRNA targetting ADE2 (crADE2-3.S)

Depositor

Jean-Marc Daran

Publication

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

crADE2-3.S (ADE2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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mRFP1-C1

Plasmid

#54764

Purpose

Localization: C1 Cloning Vector, Excitation: 584, Emission: 607

Depositor

Robert Campbell, Michael Davidson, Roger Tsien

Publication

Campbell et al Proc Natl Acad Sci U S A. 2002 Jun 11;99

Type

Empty backbone

Tags

mRFP1

Expression

Mammalian

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pCS2-UTX-F-MT2

Plasmid

#40619

Purpose

Expression of enzyme-dead UTX in mammalian cells

Depositor

Kai Ge

Publication

Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

UTX (KDM6A Human)

Tags

6xMyc and Flag

Expression

Mammalian

Mutation

changed Histidine 1146 and Glutamic Acid 1148 to …

Promoter

CMV

Available since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pET32a-HD46Q

Plasmid

#11515

Depositor

Pamela Bjorkman

Publication

Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

Insert

huntingtin, exon 1, 46Q glutamines (HTT Human)

Tags

His, His (out of frame), and Trx

Expression

Bacterial

Mutation

huntingtin exon 1 with 46 glutamines, vector m…

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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SpyLigase

Plasmid

#51722

Purpose

Plasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!

Depositor

Mark Howarth

Publication

Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.

Insert

SpyLigase

Tags

His6 Tag

Expression

Bacterial

Promoter

T7

Available since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAdTrack-CMV FLAG Rev-Erb alpha

Plasmid

#22745

Depositor

Bruce Spiegelman

Publication

Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

Insert

Rev Erb alpha (NR1D1 Human)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Available since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG-HcRed

Plasmid

#11152

Purpose

Mammalian expression vector for expression of HcRed

Depositor

Connie Cepko

Publication

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Insert

HcRed1 from Heteractis crispa

Expression

Mammalian

Available since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pBabe human FOXC2

Plasmid

#15535

Depositor

Bob Weinberg

Publication

Mani et al Proc Natl Acad Sci U S A. 2007 Jun 12. 1

Insert

FOXC2 (FOXC2 Human)

Use

Retroviral

Expression

Mammalian

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pCAG-Fab_8D3_2_H-H6

Plasmid

#176075

Purpose

Express His6 tagged heavy chain of Fab_8D3_2 in mammalian cells

Depositor

Tom Rapoport

Publication

Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

His6 tagged heavy chain of Fab_8D3_2

Tags

His tag

Expression

Mammalian

Promoter

CAG promoter

Available since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Fab_8D3_2_L

Plasmid

#176076

Purpose

Express light chain of Fab_8D3_2 in mammalian cells

Depositor

Tom Rapoport

Publication

Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

Light chain of Fab_8D3_2

Expression

Mammalian

Promoter

CAG promoter

Available since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCFD1-dU6:1gRNA

Plasmid

#49408

Purpose

expression of gRNA under control of the Drosophila U6:1 promoter

Depositor

Simon Bullock

Publication

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Insert

dU6-1:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6-1

Available since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pHR' CMV GFP

Plasmid

#14858

Depositor

Inder Verma

Publication

Miyoshi et al Proc Natl Acad Sci U S A. 1997 Sep 16. 9

Insert

CMV-GFP

Use

Lentiviral

Expression

Mammalian

Available since

May 11, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pEP0016_pIVTRup(BsmBI)_BSD

Plasmid

#242546

Purpose

IVT

Depositor

Christoph Bock, Paul Datlinger

Publication

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

BSD (Blasticidin S deaminase)

Use

Other

Available since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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MAC-CTSS

Plasmid

#172415

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Cathepsin S (CTSS Human)

Tags

MAC-tag

Expression

Mammalian

Promoter

CMV promoter

Available since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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