We narrowed to 13,954 results for: Cre

Showing: 3461 - 3480 of 13954 results

Results per page:

Sort results by:

pET15b T7pCONS TIR-2 sfGFP

Plasmid

#154468

Purpose

pET15b with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFP

Depositor

Daniel Daley

Publication

Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

Insert

super folder GFP

Tags

His-thrombin

Expression

Bacterial

Promoter

T7

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9(BB)-T2A-HygR

Plasmid

#118153

Purpose

SpCas9 expression vector

Depositor

Jorge Ferrer

Publication

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hSpCas9-T2A-HygR

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Promoter

CAG and U6

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pOT_7-lenti-EFS-SS1-BBz-2A-puro

Plasmid

#217990

Purpose

Expresses anti-Mesothelin CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A for lentiviral delivery.

Depositor

Neville Sanjana

Publication

Xue et al bioRxiv [Preprint]. 2024 Nov 27:2024.11.

Insert

SS1-BBz CAR

Use

Lentiviral

Available since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GAL4-DrBphP

Plasmid

#158986

Purpose

Expresses DrBphP fused with an N-terminal GAL4 DNA binding domain in mammalian cells

Depositor

Liangcai Gu

Publication

Huang et al ACS Synth Biol. 2020 Nov 12. doi: 10.102

Insert

GAL4-DrBphP

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-APEX2-P2A-mKate2

Plasmid

#67671

Purpose

Multisite gateway entry clone for adding C-terminal fusions of APEX2-P2A-mKate2

Depositor

Rob Parton

Publication

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-P2A-mKate2

Use

Gateway multisite 3' entry clone

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR221-FGFR4

Plasmid

#60531

Purpose

Entry vector for human FGFR4

Depositor

John Brognard

Publication

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

FGFR4 (FGFR4 Human)

Use

Gateway Cloning

Mutation

c2134a P712T

Promoter

NA

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1.Gα12-LgB115

Plasmid

#134363

Purpose

Nanoluc complementation assay. Expression of Gα12 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 115 and 116 of Gα12.Addition of the HA epitope at N terminus of Gα12.

Depositor

Julien Hanson

Publication

Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Gα12-LgB115 (GNA12 Human)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

T7

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPR - EGFP sgRNA 6

Plasmid

#51765

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

Depositor

Feng Zhang

Publication

Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

Inserts

Cas9
Puromycin resistance
EGFP sgRNA 6

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EFS and U6

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
PKCalpha.K/N.FLAG

Plasmid

#10806

Depositor

Alex Toker

Publication

Hodges et al Adv Exp Med Biol. 2002 . 506(Pt A):237-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PKC alpha (PRKCA Bovine)

Tags

FLAG

Expression

Mammalian

Mutation

Kinase dead mutant: K368N (Lys 368 to Asn)

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
Cas9-PCV

Plasmid

#123644

Purpose

Expresses Cas9 with a C-terminal HUH-tag called PCV2 in E. coli

Depositor

Wendy Gordon

Publication

Aird et al Commun Biol. 2018 May 31;1:54. doi: 10.1

Inserts

PCV2 (ORF1 )
Cas9

Tags

6xHis and SUMO

Expression

Bacterial

Mutation

2-116

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDA_526

Plasmid

#184859

Purpose

Lentiviral vector that enables constitutive sgRNA expression. Also encodes Thy1.1 as a marker of transduction.

Depositor

Arlene Sharpe

Publication

Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.

Insert

sgRNA cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6

Available since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pOT_2 - lenti-EFS-tNGFR-2A-puro

Plasmid

#181971

Purpose

Expresses human truncated NGFR linked to puromycin resistance via P2A for lentiviral delivery.

Depositor

Neville Sanjana

Publication

Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

Insert

tNGFR (NGFR Human)

Use

Lentiviral

Mutation

truncation: aa 11-276

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pSH1/M-FGFR2-Fv-Fvls-E

Plasmid

#15286

Depositor

David Spencer

Publication

Freeman et al Cancer Res. 2003 Oct 1. 63(19):6237-43.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FGFR2 kinase, FKBP12v36 (Fgfr2 Mouse)

Tags

Myristoylation-targeting domain c-Src (14aa) and …

Expression

Mammalian

Mutation

FGFR2 cytoplasmic domain FKBP12 F36V

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-TBG-mem-TurboID

Plasmid

#149413

Purpose

Hepatocyte-specific expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Publication

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Insert

mem-TurboID

Use

AAV

Tags

HA and V5

Promoter

TBG

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNASAM(gTetO)-TREGFP-PGKpuroBFP-W

Plasmid

#112927

Purpose

CRISPR-a reporter assay lentiviral vector (gTetO)

Depositor

Kosuke Yusa

Publication

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

hU6-gRNASAM(gTetO), TREGFP, PGKpuroGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Str-KDEL_flGalT-SBP-EGFP

Plasmid

#65272

Purpose

synchronize trafficking of GalT from the ER (RUSH system)

Depositor

Franck Perez

Publication

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

galactosidase

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Promoter

CMV

Available since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
Str-KDEL_flGalT-SBP-mCherry

Plasmid

#65273

Purpose

synchronize trafficking of GalT from the ER (RUSH system)

Depositor

Franck Perez

Publication

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

galactosidase

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Promoter

CMV

Available since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCCl-MCS_miniCMV_EGFP

Plasmid

#134984

Purpose

The plasmid includes a EGFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

Depositor

Claudio Mussolino

Publication

Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

Insert

EGFP

Use

Lentiviral

Promoter

minCMV

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pEXPYR

Plasmid

#70030

Purpose

High yield protein secretion in Aspergillus nidulans

Depositor

Rolf Prade

Publication

Segato et al Enzyme Microb Technol. 2012 Jul 15;51(2)

Type

Empty backbone

Use

Synthetic Biology

Promoter

A. niger glucoamylase promoter (glaAp)4

Available since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
bU6-sgNeo1-mU6-sgLkb1_2nd-hU6-sgNT

Plasmid

#177229

Purpose

Expresses Neomycin (bU6), Lkb1 (mU6) and non-targeting(hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Insert

sgNeo1/sgLkb1_2nd/sgNT1

Use

Lentiviral

Promoter

bU6/mU6/hU6

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge