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anti-Notch1_E6-pBIOCAM5

Plasmid

#39344

Depositor

John McCafferty

Article

Falk et al Methods. 2012 Sep;58(1):69-78. doi: 10.1

Insert

scFv-Fc-fusion specific for Notch1 (clone B9) (Notch1, NOTCH1 Mouse, Human)

Tags

3xFLAG, 6xHis, and human Fc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pGP18 (CK2alpha' K69M)

Plasmid

#27090

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha' K69M (CSNK2A2 Human)

Tags

HA

Expression

Mammalian

Mutation

K69M

Promoter

CMV

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only
pEMS1982

Plasmid

#49113

Purpose

AAV plasmid with CLDN5 (Ple261) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Baumann et al Exp Eye Res. 2019 Jul 16:107728. doi: 10

Insert

ssAAV-Ple261-iCre

Use

AAV

Expression

Mammalian

Promoter

CLDN5

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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CMV/N3IC-HA

Plasmid

#47618

Purpose

in vitro transcription and translation of HA-tagged Notch3 Intracellular domain

Depositor

Urban Lendahl

Article

Lardelli et al Mech Dev. 1996 Oct;59(2):177-90.

Insert

Notch3 Intracellular domain (Notch3 Mouse)

Use

In vitro transcription and translation

Tags

HA

Expression

Mammalian

Mutation

codons 1664 (Met) to 2318 (Ala)

Promoter

CMV

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
UBC plasmid

Plasmid

#169746

Purpose

Unregulated "open-loop" UBC promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

EGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

UBC

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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Red Glifon 3000

Plasmid

#163116

Purpose

Red fluorescent protein-based glucose sensor for mammalian cells with lower affinity

Depositor

Takashi Tsuboi

Article

Mita et al Cell Chem Biol. 2021 Jun 29. pii: S2451-

Insert

Red Glifon 3000

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV CMV GCGR-V5-sTurboID (N)

Plasmid

#153009

Purpose

Expresses split-TuboID N-terminal fragment fused to GCGR

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

GCGR-sTurboID (N) (L73)

Use

AAV

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmaxAW

Plasmid

#125647

Purpose

Expresses ABEmaxAW in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A, TadA* V106W)

Expression

Mammalian

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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double cut NH-donor

Plasmid

#83576

Purpose

eGFP reporter donor containing double SA cutting sites for NHEJ knock-in

Depositor

Bo Feng

Article

He et al Nucleic Acids Res. 2016 May 19;44(9):e85

Insert

eGFP

Use

Nhej donor

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPguCas13b-EGFP-NLS-3xFlag

Plasmid

#132400

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPguCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H151A; H1121A

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-ECFPEYFP

Plasmid

#24520

Depositor

Liusheng He, Jehonathan Pinthus

Article

He et al Cytometry A. 2003 Oct . 55(2):71-85.

Insert

ECFP, EYFP

Tags

ECFP and EYFP

Expression

Mammalian

Available Since

June 3, 2011

Availability

Academic Institutions and Nonprofits only
SuperNova Green/ pRSETB

Plasmid

#124839

Purpose

bacterial expression of SuperNovaGreen, a green monomeric photosensitizing fluorescent protein

Depositor

Takeharu Nagai

Article

Riani et al BMC Biol. 2018 Apr 30;16(1):50. doi: 10.

Insert

SuperNova Green

Tags

No tag

Expression

Bacterial

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pRRL-VinculinTS

Plasmid

#111830

Purpose

Vinculin tension sensor in lentiviral expression vector

Depositor

Brenton Hoffman

Article

Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

Insert

VinculinTS (VCL Chicken)

Use

Lentiviral

Promoter

CMV

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-2xFYVE_hrs

Plasmid

#67676

Purpose

Multisite gateway entry clone for adding C-terminal fusions of 2xFYVE domain from hrs

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

2xFYVEhrs

Use

Gateway multisite 3' entry clone

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pSGFP2-C1

Plasmid

#22881

Depositor

Dorus Gadella

Article

Kremers et al Biochemistry. 2007 Mar 27. 46(12):3775-8

Insert

SGFP2

Expression

Mammalian

Available Since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only

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pNL-EGFP/CMV/WPREdU3

Plasmid

#17579

Depositor

Jakob Reiser

Article

Ricks et al Stem Cells Dev. 2008 Jun . 17(3):441-50.

Insert

EGFP

Use

Lentiviral

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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LRG2.1-TagBFP2

Plasmid

#124773

Purpose

Lentiviral plasmid to co-express a guide RNA and TagBFP2

Depositor

Jason Sheltzer

Article

Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Tfap2a

Plasmid

#70274

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Tfap2a

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Tfap2a (Tfap2a Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only
Src-EGFP

Plasmid

#110496

Purpose

Fluorescent Src

Depositor

Terence Dermody, Bernardo Mainou

Article

Mainou et al J Virol. 2011 Apr;85(7):3203-13. doi: 10

Insert

c-Src (Src Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Gata6

Plasmid

#72694

Purpose

Doxycyline regulated expression of Gata6 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Gata 6 (Gata6 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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