We narrowed to 4,433 results for: chm

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NFATc1-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75236

Purpose

CRISPR/Cas9 plasmid against human NFATc1

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pMIG-hH4-AVITEV

Plasmid

#74051

Purpose

retroviral expression plasmid for human histone H4 with C-terminal BirA-biotinylation signal and TEV celavage site

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

human Histone-H4 (H4C16 Human)

Use

Retroviral

Tags

AVI-TEV

Expression

Mammalian

Promoter

pMSCV-LTRs

Available Since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

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attB-GFP-Tet1-Poly(A)

Plasmid

#65559

Purpose

Plasmid for Bxb1-mediated recombination of the GFP-Tet1 cDNA into a MIN-tagged locus

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Tet1 (Tet1 Mouse)

Use

Mouse Targeting; Bxb1

Tags

GFP

Expression

Mammalian

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-p35S-GFP(rc)13

Plasmid

#127526

Purpose

Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 13 attachment sites.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

EGFP reverse complement coding sequence flanked by attB/attP Integrase 13 attachment sites.

Expression

Plant

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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DDX27-1

Plasmid

#155514

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DDX27 (DDX27 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1-V1251R_R

Plasmid

#147376

Purpose

Mammalian Expression of HsNot1iso1-V1251R

Depositor

Elisa Izaurralde

Article

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1-V1251R (CNOT1 Human)

Expression

Mammalian

Mutation

A deletion of 5 AA 822-827 SKMKPS-> T and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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NFATc1-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75237

Purpose

CRISPR/Cas9 plasmid against human NFATc1

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.CD86.C-HA

Plasmid

#154848

Purpose

Expresses murine CD86 protein (T-lymphocyte activation antigen) with HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Cluster of Differentiation 86 (Cd86 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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attB-mCh-Dnmt3b6-Poly(A)-NeoR

Plasmid

#65554

Purpose

Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b6 cDNA into a MIN-tagged locus using Neomycin selection

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Dnmt3b (Dnmt3b Mouse)

Use

Mouse Targeting; Bxb1

Tags

mCherry

Expression

Mammalian

Mutation

isoform 6

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pGBT-RP8.1

Plasmid

#189846

Purpose

Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Lens tagBFP frameshift 1.

Depositor

Stephen Ekker

Article

Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

Type

Empty backbone

Use

Cre/Lox

Tags

mRFP and tagBFP

Promoter

beta Actin

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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CRKRS

Plasmid

#155484

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CRKRS (CDK12 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

K965R and P1269L mutation compared to NP_055898.1

Promoter

EF-1a

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_LN-HA-AGO2-5xA

Plasmid

#92010

Purpose

Expresses Lambda-N-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Tags

Lambda-N-HA

Expression

Mammalian

Mutation

S824A, S828A, T830A, S831A, S834A (5xA)

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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ELMOD3

Plasmid

#155584

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

ELMOD3 (ELMOD3 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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DHX40

Plasmid

#155540

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DHX40 (DHX40 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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RBBP6

Plasmid

#155879

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

RBBP6 (RBBP6 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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CCNL2

Plasmid

#155441

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CCNL2 (CCNL2 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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CCNL1

Plasmid

#155440

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CCNL1 (CCNL1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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BTG3

Plasmid

#155427

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

BTG3 (BTG3 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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EXOSC2

Plasmid

#155590

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

EXOSC2 (EXOSC2 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.351v2/wtRBD

Plasmid

#169467

Purpose

Encodes SARS-CoV-2 B.1.351v2/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.351v2/wtRBD Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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