We narrowed to 59,352 results for: cloning

1. LC701: pMVP (L3-L2) eCFP + polyA

   Plasmid

   #121767

   Purpose

   pMVP L3-L2 entry plasmid, contains eCFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eCFP fusion to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   eCFP + polyA
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. KO901: pMVP (L5-L4) Blast-P2A

   Plasmid

   #121715

   Purpose

   pMVP L5-L4 entry plasmid, contains Blast-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Blast selection marker linked by P2A to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   Blast-P2A (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. KP101: pMVP (L5-L4) Puro-P2A

   Plasmid

   #121716

   Purpose

   pMVP L5-L4 entry plasmid, contains Puro-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Puro selection marker linked by P2A to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   Puro-P2A (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. KP001: pMVP (L5-L4) Neo-P2A

   Plasmid

   #121717

   Purpose

   pMVP L5-L4 entry plasmid, contains Neo-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Neo selection marker linked by P2A to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   Neo-P2A (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. KK802: pMVP (L5-L4) Zeo-P2A

   Plasmid

   #121719

   Purpose

   pMVP L5-L4 entry plasmid, contains Zeo-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Zeo selection marker linked by P2A to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   Zeo-P2A (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. IH001: pMVP (L5-L4) HA epitope tag

   Plasmid

   #121699

   Purpose

   pMVP L5-L4 entry plasmid, contains HA epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   HA epitope tag (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. IG901: pMVP (L5-L4) FLAG epitope tag

   Plasmid

   #121701

   Purpose

   pMVP L5-L4 entry plasmid, contains FLAG epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   FLAG epitope tag (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. IH101: pMVP (L5-L4) OLLAS epitope tag

   Plasmid

   #121703

   Purpose

   pMVP L5-L4 entry plasmid, contains OLLAS epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   OLLAS epitope tag (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. IX104: pMVP (L5-L4) myc epitope tag

   Plasmid

   #121704

   Purpose

   pMVP L5-L4 entry plasmid, contains myc epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   myc epitope tag (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. IX203: pMVP (L5-L4) V5 epitope tag

    Plasmid

    #121705

    Purpose

    pMVP L5-L4 entry plasmid, contains V5 epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    V5 epitope tag (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. KP401: pMVP (L5-L4) eCFP-P2A

    Plasmid

    #121712

    Purpose

    pMVP L5-L4 entry plasmid, contains eCFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eCFP linked by P2A to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    eCFP-P2A (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223 LC3B G120A no-stop

    Plasmid

    #123206

    Purpose

    Gateway entry clone encoding human MAP1LC3B G120A lacking stop codon, suitable for C-terminal tagging

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Glycine 120 to Alanine, stop codon removed

    Available Since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223 GABARAPL1 no-stop

    Plasmid

    #123210

    Purpose

    Gateway entry clone encoding human GABARAPL1 lacking stop codon, suitable for C-terminal tagging

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Stop codon removed

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223 GABARAPL2 no-stop

    Plasmid

    #123211

    Purpose

    Gateway entry clone encoding human GABARAPL2 lacking stop codon, suitable for C-terminal tagging

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Stop codon removed

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMOD_B3200

    Plasmid

    #91079

    Purpose

    Module B, Promoter: none (begins with P2A to be fused to TALE-VPR_1), Gene: TALE-VPR_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TALE-VPR_2 backbone with Esp3I ccdb cassette for repeat cloning
    

    Use

    TALEN

    Promoter

    none (begins with P2A to be fused to TALE-VPR_1)

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMOD_B3400

    Plasmid

    #91080

    Purpose

    Module B, Promoter: none (begins with P2A to be fused to TALE-VP64_1), Gene: TALE-VP64_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TALE-VP64_2 backbone with Esp3I ccdb cassette for repeat cloning
    

    Use

    TALEN

    Promoter

    none (begins with P2A to be fused to TALE-VP64_1)

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMOD_A3101

    Plasmid

    #91048

    Purpose

    Module A, Promoter: 35S, Gene: TALE-VPR_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALE-VPR_ 2)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TALE-VPR_1 backbone with Esp3I ccdb cassette for repeat cloning
    

    Use

    TALEN

    Promoter

    35S

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMOD_A3301

    Plasmid

    #91049

    Purpose

    Module A, Promoter: 35S, Gene: TALE-VP64_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALE-VP64_ 2)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TALE-VP64_1 backbone with Esp3I ccdb cassette for repeat cloning
    

    Use

    TALEN

    Promoter

    35S

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. lucMAPT-GenRep

    Plasmid

    #214674

    Purpose

    MAPT Exon 10 reporter, with Exon 10 and 100bp flanking intronic regions replaced with a BamHI/EcoRI cloning site for testing different exons

    Depositor

    Eugene Yeo

    Article

    Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    MAPT (MAPT Human)
    

    Tags

    Firefly Luciferase, Renilla Luciferase, and V5

    Expression

    Mammalian

    Mutation

    Contains Exon 9 and Exon 11, with a BamHI EcoRI c…

    Promoter

    CMV

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pICH41780

    Plasmid

    #48019

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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