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We narrowed to 9,474 results for: BLI;

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Showing: 6961 - 7000 of 9474 results

  1. pALS3-Ma-sfGFP-TAG150

    Plasmid
    #212121
    Purpose
    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-TAG150 and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    sfGFP-150TAG
    M. alvus Pyl-tRNA(6)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    N150TAG
    Promoter
    araC and lpp
    Available Since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212121-plasmid-map-sequence-id-444186

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  2. pBK-Ma pylRS nitroY/haloY-F5

    Plasmid
    #212125
    Purpose
    Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control for Ma Pyl tRNA-synthetase selections.
    Depositor
    Ryan Mehl
    Insert
    M. alvus PylRS 3-NY/HaloY F5
    Expression
    Bacterial
    Mutation
    CTG125CTT, N166A V168S, A223C, W239R
    Promoter
    GlnS (constitutive)
    Available Since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    212125-plasmid-map-sequence-id-443626

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  3. SARS-CoV-2 Spike-d18

    Plasmid
    #164583
    Purpose
    A mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.
    Depositor
    Jason Sheltzer
    Insert
    SARS-CoV-2 Spike (S SARS-CoV-2)
    Expression
    Mammalian
    Mutation
    Deleted amino acids 1256-1273
    Promoter
    EF1a
    Available Since
    Jan. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    164583-plasmid-map-sequence-id-322362

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  4. pB6-Gfap-T2A-tdTomato-FNF-DTA

    Plasmid
    #140271
    Purpose
    B6J Gfap-T2A-tdTomato allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    Gfap (Gfap Mouse)
    Use
    Mouse Targeting
    Tags
    C-terminal fusion of T2A-tdTomato
    Expression
    Mammalian
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    140271-plasmid-map-sequence-id-317910

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  5. ACACA

    Plasmid
    #39083
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ACACA (ACACA Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  6. Hs.KRAS4b G13D

    Plasmid
    #83133
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G13D mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Mutation
    G13D
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83133-plasmid-map-sequence-id-158096

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  7. pJCX3-ACTG2_R257C

    Plasmid
    #228911
    Purpose
    Expresses mutant R257C smooth muscle actin gamma-2 in human cells
    Depositor
    Roberto Dominguez
    Insert
    Actin gamma 2, smooth muscle (ACTG2 Human)
    Tags
    HIS-FLAG-TEV
    Expression
    Bacterial and Mammalian
    Mutation
    R257C
    Promoter
    CMV
    Available Since
    Jan. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    228911-plasmid-map-sequence-id-457665

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  8. pJCX3-ACTG2_WT

    Plasmid
    #228910
    Purpose
    Expresses wildtype smooth muscle actin gamma-2 in human cells
    Depositor
    Roberto Dominguez
    Insert
    Actin gamma 2, smooth muscle (ACTG2 Human)
    Tags
    HIS-FLAG-TEV
    Expression
    Bacterial and Mammalian
    Mutation
    WT
    Promoter
    CMV
    Available Since
    Jan. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    228910-plasmid-map-sequence-id-457664

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  9. Hs.KRAS4b G12C

    Plasmid
    #83130
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12C mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Mutation
    G12C
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83130-plasmid-map-sequence-id-158093

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  10. AICSDP-69: NPM1-mTagRFP-T

    Plasmid
    #124608
    Purpose
    Homology arms and linker-mTagRFP-T sequence for C-terminus tagging of human NPM1
    Depositor
    Allen Institute for Cell Science
    Insert
    NPM1 Homology Arms with linker-mTagRFP-T (NPM1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    May 13, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    124608-plasmid-map-sequence-id-244304

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  12. Hs.KRAS4a G12D

    Plasmid
    #83168
    Purpose
    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G12D mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Mutation
    G12D
    Available Since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83168-plasmid-map-sequence-id-158131

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  13. Lamin A (G465D)-mRFP

    Plasmid
    #124271
    Purpose
    Lamin A mutant (G465D), C-terminally tagged with mRFP. Mutation associated with FPLD lipodystrophy.
    Depositor
    Eric Schirmer
    Insert
    LMNA (G465D) (LMNA Human)
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    LMNA (G465D)
    Available Since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124271-plasmid-map-sequence-id-241962

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  16. pAAV-hSyn-DIO-HA-rM3D(Gs)-IRES-mCitrine

    Plasmid
    #50456
    Purpose
    Double floxed Gs-coupled rM3D-IRES-mCitrine under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Insert
    rM3D-IRES-mCitrine (CHRM3 Human)
    Use
    AAV
    Tags
    HA
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available Since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50456-plasmid-map-sequence-id-75403

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  19. 4IKP

    Plasmid
    #60080
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CARM1:JMC049-A06:C226836 (CARM1 Human)
    Use
    Baculovirus expression
    Tags
    6xHis
    Mutation
    See comments
    Promoter
    polyhedrin
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)

    Plasmid
    #66708
    Purpose
    expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed
    Depositor
    Charles Gersbach
    Insert
    S.Pyogenes VP64-dCas9-VP64
    Use
    Lentiviral and Synthetic Biology
    Tags
    VP64
    Mutation
    D10A and H840A
    Promoter
    hUbC
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    66708-plasmid-map-sequence-id-449286

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  21. pOET5 CVB3-VLP

    Plasmid
    #218146
    Purpose
    Forms coxsackievirus 3-like particle in insect cells by expression of VP1–VP4 polyprotein (GeneID M33854.1) and 3CD protease (GeneID M33854.1).
    Depositor
    Minna Hankaniemi
    Inserts
    VP1–VP4
    3CD
    Expression
    Insect
    Promoter
    CMV and Polyhedrin
    Available Since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218146-plasmid-map-sequence-id-433321

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  22. pCDF1-mAtf4-HT-puro

    Plasmid
    #101795
    Purpose
    Lentiviral vector containing C-terminally Halo-tag tagged mouse ATF4
    Depositor
    Seiichi Oyadomari
    Insert
    Activating transcription factor 4 (Atf4 Mouse)
    Use
    Lentiviral
    Tags
    Halo tag
    Expression
    Mammalian
    Available Since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    101795-plasmid-map-sequence-id-194514

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  24. Hs.NRAS G12V

    Plasmid
    #83174
    Purpose
    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), G12V mutation
    Depositor
    Dominic Esposito
    Insert
    neuroblastoma RAS viral oncogene homolog (NRAS Human)
    Use
    Gateway entry clone
    Mutation
    G12V
    Available Since
    Sept. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    83174-plasmid-map-sequence-id-158137

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  25. Hs.KRAS4b G12S

    Plasmid
    #83144
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12S mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Mutation
    G12S
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83144-plasmid-map-sequence-id-158107

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  26. pET28a-UBC9

    Plasmid
    #228949
    Purpose
    express a fragment of the wild-type human UBC9 (residues 2-158 of UniProt P63279 supplemented with the N-terminal thrombin-cleavable MGHHHHHHSSGLVPR|G- tag)
    Depositor
    Marcin Suskiewicz
    Insert
    UBC9 (UBE2I Human)
    Tags
    His tag and thrombine cleavage site : MGHHHHHHSSG…
    Expression
    Bacterial
    Promoter
    T7-lac
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    228949-plasmid-map-sequence-id-472392

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  28. pAAV-hSyn-GluA1-scFv-mNeon-KDEL

    Plasmid
    #230056
    Purpose
    AAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 tagged with mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.
    Depositor
    Matthew Kennedy
    Insert
    Anti-GluA1 single chain variable fragment (scFv)
    Use
    AAV
    Tags
    ER retrieval motif (KDEL) and mNeon
    Expression
    Mammalian
    Promoter
    human synapsin
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230056-plasmid-map-sequence-id-457171

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  29. pTRE-T2-IRES-His6-Ubiquitin

    Plasmid
    #107107
    Purpose
    Designed to co-express target gene upstream of the IRES element at the multiple cloning site with downstream His6-tagged ubiquitin expressed after IRES.
    Depositor
    Michael E. Wright
    Insert
    human ubiquitin UBB (UBB Human)
    Tags
    6xHis
    Expression
    Mammalian
    Promoter
    Tet
    Available Since
    Aug. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    107107-plasmid-map-sequence-id-218027

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  30. ALDH18A1

    Plasmid
    #38947
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ALDH18A1 (ALDH18A1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  33. pAAV-TREtight-mTagBFP2-N2cG

    Plasmid
    #192838
    Purpose
    plasmid for making helper virus for monosynaptic tracing using CVS-N2c rabies virus; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA
    Depositor
    Ian Wickersham
    Insert
    mTagBFP2 and rabies virus CVS-N2c strain glycoprotein genes (N2cG)
    Use
    AAV
    Expression
    Mammalian
    Promoter
    TREtight
    Available Since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    192838-plasmid-map-sequence-id-378336

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  34. JMC044-B12

    Plasmid
    #58752
    Purpose
    bacterial expression of PDB 4NW3
    Depositor
    Cheryl Arrowsmith
    Insert
    PDB:4NW3 (KMT2A Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available Since
    Aug. 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  35. MS2_SC2_S_Delta

    Plasmid
    #175950
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Delta SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Expression
    Bacterial
    Mutation
    Delta (Amino Acid 319-869)
    Promoter
    T7
    Available Since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    175950-plasmid-map-sequence-id-345521

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  36. PE-SUMO CRYAB

    Plasmid
    #217670
    Purpose
    For expression of SUMO tagged human alpha B crystallin
    Depositor
    Larry David
    Insert
    CRYAB (CRYAB Human)
    Tags
    6XHis and Smt3
    Expression
    Bacterial
    Available Since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    217670-plasmid-map-sequence-id-434869

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  37. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

    Plasmid
    #201151
    Purpose
    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.
    Depositor
    Moshe Szyf
    Inserts
    SadCas9-KRAB
    gRNA-TRE
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Mutation
    gRNA sequence: ATCAGTGATAGAGAACGTATG
    Available Since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    201151-plasmid-map-sequence-id-401792

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  38. pAAV-hSyn-GluA1-scFv-Halo

    Plasmid
    #230055
    Purpose
    AAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 fused to halotag. Can be directly transfected into neurons or used to prepare AAV expressing GluA1-scFv-Halo.
    Depositor
    Matthew Kennedy
    Insert
    Anti-GluA1 single chain variable fragment (scFv)
    Use
    AAV
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    human synapsin
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230055-plasmid-map-sequence-id-457173

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  39. pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s

    Plasmid
    #105715
    Purpose
    In the presence of Cre, the plasmid can be used to express GCaMP6s fused to the presynaptic protein synaptophysin. Drives enriched expression of GCaMP6s in neuronal axon boutons.
    Depositor
    Rylan Larsen
    Has Service
    AAV5
    Insert
    synaptophysin-GCaMP6s
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    Ef1a, Human elongation factor-1 alpha
    Available Since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    105715-plasmid-map-sequence-id-207274

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  40. pScDD2_ERdd

    Plasmid
    #109047
    Purpose
    destabilizing domains for yeast (S. cerevisiae) derived from the human estrogen receptor ligand binding domain. This ERdd can be stabilized with 10 uM 4-hydroxytamoxifen (4-OHT, OHTAM).
    Depositor
    Thomas Wandless
    Insert
    ER50 (ESR1 H. sapiens gene with yeast enhanced codon)
    Tags
    yeast enhanced GFP with 8 amino acids TSGRGEGQ li…
    Expression
    Yeast
    Mutation
    3 mutations : E380G, R434G, N532S
    Promoter
    SOD1
    Available Since
    April 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    109047-plasmid-map-sequence-id-209173

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Showing: 6961 - 7000 of 9474 results