We narrowed to 4,223 results for: 424

1. NES-mCherry-PH-BTKx2

   Plasmid

   #183654

   Purpose

   PtdIns(3,4,5)P3 biosensor

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   BTK (BTK Human, Frog)
   

   Use

   Tags

   X. laevis map2k1.L(32-44)-mCherry

   Expression

   Mammalian

   Mutation

   Amino acids 2-174 and 2-170

   Promoter

   CMV

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q66

   Plasmid

   #111750

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q66 (polyQ repeat)

   Promoter

   CMV and P10

   Available since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q73

   Plasmid

   #111724

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q73 (polyQ repeat)

   Promoter

   CMV and P10

   Available since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSbi-pur-EGFP-KSR1-CA3 (N-KSR)

   Plasmid

   #217769

   Purpose

   Fluorescent reporter for glucosyl-ceramide (putative, mammalian expression)

   Depositor

   Paula Nunes-Hasler

   Article

   Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

   Insert

   Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
   

   Use

   Sleeping beauty transposase-compatible genome ins…

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   CA3 domain aa 317-400

   Promoter

   EF-1alpha promoter

   Available since

   June 10, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pRSFDuet-FBXL5-SKP1

   Plasmid

   #226620

   Purpose

   Co-expression of FBXL5-SKP1 complex in E.coli

   Depositor

   Ning Zheng

   Article

   Wang et al Mol Cell. 2020 Apr 2;78(1):31-41.e5. doi

   Inserts

   FBXL5 (FBXL5 Human)
   SKP1 (SKP1 Human)
   

   Use

   Tags

   GB1

   Expression

   Bacterial

   Mutation

   Amino acids 1-198 was deleted, and 420-596 was re…

   Promoter

   T7

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. mRFP-FKBP12-5ptpase domain

   Plasmid

   #67516

   Purpose

   Recruitable human type IV 5-phosphatase domain (214-644)

   Depositor

   Tamas Balla

   Article

   Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

   Insert

   FKBP12-5ptpase enzyme (INPP5E, FKBP1A Human)
   

   Use

   Tags

   mRFP

   Expression

   Mutation

   INPP5E has C641A to destroy the C-terminal CAAX d…

   Promoter

   Available since

   Sept. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q73

   Plasmid

   #111728

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q73 (polyQ repeat)

   Promoter

   CMV and P10

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXs-GNL

   Plasmid

   #188232

   Purpose

   Polycistronic human Glis1, LIN28 and NANOG were cloned into the pMXs vector

   Depositor

   Young Tang

   Article

   Wang et al Biol Open. 2019 Dec 5;8(12). pii: 8/12/b

   Insert

   Glis1, NANOG and LIN28A separated by 2A sequence (GLIS1, NANOG, LIN28A Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-PD-1ecto&tmd-BTLAicd-mGFP

   Plasmid

   #180794

   Purpose

   Expression of human PD-1 extracellular domain and transmembrane domain fused to BTLA intracellular domain followed by mEGFP

   Depositor

   Enfu Hui

   Article

   Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   PD-1ecto&tmd-BTLAint (PDCD1, BTLA Human)
   

   Use

   Lentiviral

   Tags

   mEGFP

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223_NRAS_p.Q61K

    Plasmid

    #81657

    Purpose

    Gateway Donor vector containing NRAS, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    NRAS (NRAS Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Q61K

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223_STAT1_WT

    Plasmid

    #82234

    Purpose

    Gateway Donor vector containing STAT1, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    STAT1 (STAT1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1-TRC.mKO2_shB3GNT5.1

    Plasmid

    #110325

    Purpose

    TRCN0000034809 (Target GCCTATGTAATCTCCGGTGAT), silence human B3GNT5 gene and express monomeric Kusabira-Orange2

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    B3GNT5 (B3GNT5 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKSR1-CA3-EGFP (C-KSR)

    Plasmid

    #217753

    Purpose

    Fluorescent reporter for ceramide (mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CA3 domain aa 317-400 plus vector-based linker LE…

    Promoter

    CMV

    Available since

    June 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pMXS-IRES-BLAST EGFP-flag-CAD

    Plasmid

    #188118

    Purpose

    Expresses N-terminal EGFP-tagged/C-terminal flag-tagged human CAD in mammalian cells

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    EGFP tag and Flag tag

    Expression

    Mammalian

    Mutation

    TCC -> AGT silent mutations at nt439-441 elimi…

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKSR1-CA3-mRFP1 (C-KSR)

    Plasmid

    #217754

    Purpose

    Fluorescent reporter for ceramide (mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    CA3 domain aa 317-400 plus vector-based linker LE…

    Promoter

    CMV

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSbi-pur-KSR1-CA3-EGFP (C-KSR)

    Plasmid

    #217768

    Purpose

    Fluorescent reporter for ceramide (for stable mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    

    Use

    Sleeping beauty transposase-compatible genome ins…

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CA3 domain aa 317-400 plus vector-based linker LE…

    Promoter

    EF-1alpha promoter

    Available since

    June 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pETM11-SUMO3- sfGFP-KSR1-CA3 (N-KSR)

    Plasmid

    #217767

    Purpose

    Fluorescent reporter for glucosyl-ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    

    Use

    Protein purification (6xhis tag, tev site, sumo3 …

    Tags

    sfGFP

    Expression

    Bacterial

    Mutation

    CA3 domain aa 317-400

    Promoter

    T7 promoter

    Available since

    June 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pETM11-SUMO3-KSR1-CA3-sfGFP (C-KSR)

    Plasmid

    #217766

    Purpose

    Fluorescent reporter for ceramide (for bacterial expression and purification)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    

    Use

    Protein purification (6xhis tag, tev site, sumo3 …

    Tags

    sfGFP

    Expression

    Bacterial

    Mutation

    CA3 domain aa 317-400 plus vector-based linker LE…

    Promoter

    T7 promoter

    Available since

    June 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. BCL6-GFP G55A in Artichoke

    Plasmid

    #169935

    Purpose

    Overexpression of BCL6-GFP G55A mutant fusion

    Depositor

    Benjamin Ebert

    Article

    Slabicki et al Nature. 2020 Dec;588(7836):164-168. doi:

    Insert

    BCL6 G55A (BCL6 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    160R>C

    Promoter

    Available since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL3-sgRANGAP.C1-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid

    #216249

    Purpose

    Express Cas9 with CAG promoter to improve the expression in hES cells and sgRANGAP.C1 to tag endogenous RANGAP1 C-terminus

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    Cas9 and sgRANGAP.C1 (RANGAP1 Human)
    

    Use

    CRISPR; Endogenous tagging

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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