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AAV_CMV-AcrIIA5_cpGR2(GGS5)-N77

Plasmid

#246048

Purpose

Expression of AcrIIA5-cpGR2 hybrid in mammalian cells; insertion before N77; enables chemogenetic control of SauCas9; contains ITRs for AAV packaging with Rep2

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA5_cpGR2-N77

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV6 FLAG-DNAJC5 L115R

Plasmid

#246742

Purpose

Mammalian expression of human DNAJC5 with L115R mutation, and a FLAG tag on its N-terminus

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

DNAJC5 (DnaJ heat shock protein family) (DNAJC5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

L115R

Promoter

CMV

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV6 FLAG-DNAJC5 delL116

Plasmid

#246741

Purpose

Mammalian expression of human DNAJC5 with L115R mutation, and a FLAG tag on its N-terminus

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

DNAJC5 (DnaJ heat shock protein family) (DNAJC5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

delL116

Promoter

CMV

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4-CMV-myc-XPO1

Plasmid

#242469

Purpose

Expresses human XPO1 protein with an N-terminal cMyc tag.

Depositor

Dirk Daelemans

Article

Wing et al Nat Chem Biol. 2025 Dec;21(12):2002-2013

Insert

XPO1 (XPO1 Human)

Tags

cMyc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4-CMV-myc-XPO1_V518E_F561H

Plasmid

#242470

Purpose

Expresses human XPO1 protein with an N-terminal cMyc tag. The V518E_5561H mutations disrupt ASB8 binding.

Depositor

Dirk Daelemans

Article

Wing et al Nat Chem Biol. 2025 Dec;21(12):2002-2013

Insert

XPO1 (XPO1 Human)

Tags

cMyc

Expression

Mammalian

Mutation

V518E - F561H

Promoter

CMV

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4-CMV-myc-XPO1_H10-11

Plasmid

#242471

Purpose

Expresses human XPO1 protein with an N-terminal cMyc tag. The H10-11 mutations disrupt ASB8 binding.

Depositor

Dirk Daelemans

Article

Wing et al Nat Chem Biol. 2025 Dec;21(12):2002-2013

Insert

XPO1 (XPO1 Human)

Tags

cMyc

Expression

Mammalian

Mutation

XPO1 H10-11 = E478A - H481A - V484G - N485G - T48…

Promoter

CMV

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4-CMV-FLAG-XPO1

Plasmid

#242459

Purpose

Expresses human XPO1 with an N-terminal FLAG tag for pull-down assays.

Depositor

Dirk Daelemans

Article

Wing et al Nat Chem Biol. 2025 Dec;21(12):2002-2013

Insert

XPO1 (XPO1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAG AAV CMV EGFP-HA

Plasmid

#242963

Purpose

Edit AAV plasmid expressing EGFP-HA, control for plasmid 242962

Depositor

Meghan Rego, Addgene Research Program

Insert

EGFP

Use

AAV

Tags

HA

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAG AAV CMV HA-EGFP

Plasmid

#242961

Purpose

AAV plasmid expressing HA-EGFP, control for plasmid 242960

Depositor

Meghan Rego, Addgene Research Program

Insert

EGFP

Use

AAV

Tags

HA

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAG AAV CMV EGFP

Plasmid

#242959

Purpose

AAV plasmid expressing EGFP, control for plasmid 242957

Depositor

Meghan Rego, Addgene Research Program

Insert

EGFP

Use

AAV

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAG AAV CMV Empty

Plasmid

#242957

Purpose

Empty AAV expression backbone

Depositor

Meghan Rego, Addgene Research Program

Type

Empty backbone

Use

AAV

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pFlag-CMV4-PHF13 (1-150)

Plasmid

#246096

Purpose

Expression of human PHF13 (1-150) with Flag tag in mammalian cells

Depositor

Sarah Kinkley

Article

Rossi et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

PHF13 (1-150) (PHF13 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 1 - 150

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-LAMP1-iLID-Halo

Plasmid

#247296

Purpose

Mammalian expression of LAMP1-iLID-Halo fusion for light-induced recruitment to late endosomes/lysosomes.

Depositor

Jennifer Lippincott-Schwartz

Article

Choi et al Nature. 2025 Nov 5. doi: 10.1038/s41586-

Insert

LAMP1-iLID-Halo

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-mCherry-sspB-Sec61

Plasmid

#247297

Purpose

Mammalian expression of mCherry-sspB-Sec61 fusion; for optogenetic control of protein recruitment to the ER

Depositor

Jennifer Lippincott-Schwartz

Article

Choi et al Nature. 2025 Nov 5. doi: 10.1038/s41586-

Insert

mCherry-sspB-Sec61

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pShuttle-CMV-Flag-SREBP-1c

Plasmid

#237348

Purpose

Adenoviral-SREBP-1c cleavage-activation reporter system

Depositor

Gerald Höfler

Article

Pena de la Sancha et al Commun Biol. 2025 Apr 9;8(1):588. doi: 1

Insert

SREBF1 (SREBF1 Human)

Use

Adenoviral and Mouse Targeting

Tags

Flag tag

Promoter

CMV

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-DARPin 1238_G01-YPet

Plasmid

#244039

Purpose

anti mTFP1DARPin_G01 fused to YPet

Depositor

Markus Affolter

Article

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_G01-YPet

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-DARPin 1238_E11-YPet

Plasmid

#244038

Purpose

anti mTFP1DARPin_E11 fused to YPet

Depositor

Markus Affolter

Article

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_E11-YPet

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-DARPin 1238_G01-mCherry

Plasmid

#244029

Purpose

anti mTFP1DARPin_G01 fused to mCherry

Depositor

Markus Affolter

Article

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_G01-mCherry

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-DARPin 1238_E11-mCherry

Plasmid

#244028

Purpose

anti mTFP1DARPin_E11 fused to mCherry

Depositor

Markus Affolter

Article

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

DARPin_E11-mCherry

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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NSK249 CMV-TO-SCN1A (exons 20, 21)

Plasmid

#247284

Purpose

Expresses human SCN1a exons 20, 21

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv 2025.09.18.677213

Insert

SCN1a exons 20, 21 (SCN1A Human)

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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