We narrowed to 26,261 results for: Vit

1. pLenti_EF1α-NES-Kinprola_PKA-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

   Plasmid

   #233371

   Purpose

   EF1α driven co-expression of the PKA activity recorder Kinprola_PKA fused to mEGFP and Cas9 expression through lentivirus transduction

   Depositor

   Kai Johnsson

   Article

   Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

   Inserts

   NES-Kinprola_PKA-mEGFP
   FLAG-SV40NLS-Cas9-NLS-T2A-BSD
   

   Use

   Lentiviral

   Tags

   FLAG, NES, and mEGFP

   Promoter

   EF1α

   Available Since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3.1 KSR2

   Plasmid

   #25968

   Depositor

   Rob Lewis

   Article

   Costanzo-Garvey et al Cell Metab. 2009 Nov . 10(5):366-78.

   Insert

   Kinase suppressor of Ras 2 (Ksr2 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Aug. 13, 2010

   Availability

   Academic Institutions and Nonprofits only

3. JWW-1 human chimeric antibody

   Plasmid

   #66748

   Purpose

   Expresses a rat/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 17-36. JWW-1 works in ELISA/WB/HPV Neutralization assay.

   Depositor

   Richard Roden

   Article

   Wang et al Clin Vaccine Immunol. 2015 May 13. pii:

   Inserts

   JWW-1 Heavy Chain Gamma
   JWW-1 Light Chain Kappa
   

   Expression

   Mammalian

   Mutation

   First 3 amino acids of rat light chain constant r…

   Promoter

   mEF1 and rEF1

   Available Since

   July 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFB1.HMBP.PrS.dEZH2

   Plasmid

   #220241

   Purpose

   Expression of an EZH2 catalytic site mutant in insect cells.

   Depositor

   Chen Davidovich

   Article

   Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

   Insert

   dEZH2 (EZH2 Human)
   

   Tags

   6x His, MBP

   Expression

   Insect

   Mutation

   Y731F

   Promoter

   Polyhedrin

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3.1-Kozak-SP-SNAPf-lk-EK-ADGRL3-CTF

   Plasmid

   #201217

   Purpose

   pCDNA3.1 with Kozak (GCCACC), ADGRL3 signal peptide, SNAP-tag, GS linker, and Flag-tag (EK site) ahead of ADGRL3-CTF

   Depositor

   Jonathan Javitch

   Article

   Perry-Hauser et al J Biol Chem. 2022 Dec;298(12):102594. do

   Insert

   ADGRL3 (Adgrl3 Mouse)
   

   Tags

   Flag and SNAP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNOC_dCas9_sgRNA NC

   Plasmid

   #176259

   Purpose

   pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   dead spCas9
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   luciferase

   Mutation

   H840A and D10A mutations on spCas9 to inactivate …

   Available Since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-mCherry-TSC2(AA,3Q)-NLS

   Plasmid

   #192445

   Purpose

   Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity; targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q )-NLS (TSC2 Human)
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

   Promoter

   CMV

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA4-His-Max-A-PQBP1

   Plasmid

   #25802

   Depositor

   Marius Sudol

   Article

   Tapia et al J Biol Chem. 2010 Jun 18;285(25):19391-4

   Insert

   PQBP1 (PQBP1 Human)
   

   Tags

   His and Xpress

   Expression

   Mammalian

   Available Since

   July 21, 2010

   Availability

   Academic Institutions and Nonprofits only

9. human rSHIP2 (18-893)

   Plasmid

   #128568

   Purpose

   Expression of human SHIP2 in bacteria

   Depositor

   Christophe Erneux

   Article

   Pesesse et al FEBS Lett. 1998 Oct 23;437(3):301-3.

   Insert

   SHIP2 (INPPL1 Human)
   

   Tags

   His Xpress (N-terminal)

   Expression

   Bacterial

   Mutation

   SHIP2 coding sequence from amino acid 18 (A) till…

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. KHBD00332

    Plasmid

    #34455

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG1765 (EcR Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pDEST53-LRRK2-ROC

    Plasmid

    #25053

    Depositor

    Mark Cookson

    Article

    Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

    Insert

    LRRK2 (LRRK2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Contains only the ROC domain- aa 1333-1516

    Available Since

    Nov. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

12. UPRT-mCh-Nluc-P2A-neo-UPRT

    Plasmid

    #135015

    Purpose

    Expresses mCherry protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum UPRT locus

    Depositor

    David Sibley

    Article

    Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

    Inserts

    mCh-Nluc-P2A-neo
    UPRT 5'UTR
    UPRT 3'UTR
    

    Use

    Cryptosporidium expression

    Tags

    mCherry and Nluc-neo

    Promoter

    Cryptosporidium actin and enolase

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lyn-FLINC-EKAR1(TA)

    Plasmid

    #87708

    Purpose

    Negative-control mutant of Lyn-FLINC-EKAR1.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

    Insert

    Lyn-FLINC-EKAR1(TA)
    

    Tags

    N-terminal targeting sequence from Lyn kinase

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNOC_dfnCas12a_crRNA NC

    Plasmid

    #176256

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and spacer sequence is replaced by the type IIS restriction site for endonuclease BaeI that can be

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Mutation

    D917A and E1006A mutations to inactivate the endo…

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. KHBD00202

    Plasmid

    #34353

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG33261 (Trl Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

16. KHBD00020

    Plasmid

    #34182

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG1429 (Mef2 Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA-zeo: alpha6mGFP

    Plasmid

    #35611

    Purpose

    mammalian expression of mouse Alpha6 (Chrna6) with internal mEGFP tag

    Depositor

    Henry Lester

    Article

    Xiao et al Biochem Pharmacol. 2011 Oct 15;82(8):852

    Insert

    nAChr alpha6mEGFP (Chrna6 Mouse)
    

    Tags

    mEGFP inserted after A405 with AGA linker on eith…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. KHBD00210

    Plasmid

    #34361

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG17894 (cnc Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

19. KHBD00009

    Plasmid

    #34172

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG5058 (grh Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

20. KHBD00624

    Plasmid

    #39696

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG7450 (CrebA Fly)
    

    Use

    Gateway donr vector

    Promoter

    none

    Available Since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only