We narrowed to 162,651 results for: addgene

1. pMB3-B-TelR

   Plasmid

   #204992

   Purpose

   Level 3 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

   Depositor

   Veronica Godoy-Carter

   Article

   Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

   Insert

   mRFP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Added replications elements for Acinetobacter spe…

   Promoter

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiGuide_mCherry_sgEPAS1#1

   Plasmid

   #183122

   Purpose

   pLentiGuide modified to express mCherry and an sgRNA targeting EPAS1

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   sgEPAS1-1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-LAP2-mCherry

   Plasmid

   #159524

   Purpose

   Expresses mCherry from LAP2 promoter

   Depositor

   Esteban Engel

   Article

   Maturana et al Mol Ther Methods Clin Dev. 2020 Apr 14;1

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LAP2

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAGM4723:TpCC_Urease

   Plasmid

   #85982

   Purpose

   Golden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAs

   Depositor

   Thomas Mock

   Article

   Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

   Inserts

   Cas9
   Urease-targeting gRNA 1
   Urease-targeting gRNA 2
   

   Use

   CRISPR; Diatom (t. pseudonana)

   Tags

   NLS and YFP

   Expression

   Mutation

   Promoter

   FCP and U6

   Available since

   Jan. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETDuet-LahT150

   Plasmid

   #166785

   Purpose

   Cleavage of double glycine motif in peptides to remove the leader sequence

   Depositor

   Wilfred van der Donk

   Article

   Bobeica et al Elife. 2019 Jan 14;8. pii: 42305. doi: 1

   Insert

   LahT150
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. mc2 mNeon EF1a

   Plasmid

   #206224

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Hmga2 3'UTR wt luciferase (Luc-wt)

   Plasmid

   #14785

   Depositor

   David Bartel

   Article

   Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

   Insert

   Luc-wt (Hmga2 Mouse)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 6, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDBTrp-LexABD-CRY2FL

   Plasmid

   #78210

   Purpose

   Use with Addgene 40615 for blue light transcription control in yeast

   Depositor

   Chandra Tucker

   Article

   Pathak et al ACS Synth Biol. 2014 Nov 21;3(11):832-8.

   Insert

   LexABD-CRY2 (CRY2 Mustard Weed)
   

   Use

   Tags

   lexA binding domain

   Expression

   Yeast

   Mutation

   contains endogenous CRY2 NLS

   Promoter

   ADH1

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPerimCh

   Plasmid

   #227013

   Purpose

   Expresses periplasmic mCherry

   Depositor

   Suzan Rooijakkers

   Article

   Heesterbeek et al Sci Rep. 2019 Feb 28;9(1):3074. doi: 10.

   Insert

   PelB-mCherry, arabinose inducible cytosolic GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rpsM and pBAD

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. MAC_C_PDK1

    Plasmid

    #111672

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    PDK1 (PDK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. B52_puro_empty_gRNA

    Plasmid

    #197557

    Purpose

    sites for cloning two gRNA with puromycin cassette

    Depositor

    Darius Bägli

    Type

    Empty backbone

    Use

    Empty grna backbone with puromycin resistance gene

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for gRNA, CMV for puromycin resistance.

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. NLS-tdPCP-GFP

    Plasmid

    #183934

    Purpose

    PCP tandem dimer binding to PP7 RNA stem loops; labeled with GFP

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    tdPCP-GFP
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV (TATA box removed)

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. mc2 puro EF1a

    Plasmid

    #206221

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-eDIO-EYFP

    Plasmid

    #104388

    Purpose

    PiggyBac plasmid encoding EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.

    Depositor

    Volker Busskamp

    Article

    Klapper et al Sci Rep. 2017 Oct 31;7(1):14450. doi: 10

    Insert

    Enhanced Yellow Fluorescent Protein
    

    Use

    Cre/Lox; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG2IG-Hmga1a

    Plasmid

    #122293

    Purpose

    Overexpression for mouse Hmga1a

    Depositor

    Yukiko Gotoh

    Article

    Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

    Insert

    Hmga1a (Hmga1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCOLA-Spec-yibDp-Erv1p

    Plasmid

    #212094

    Purpose

    Expresses sulfydryl oxidase (Erv1p) after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    Sulfydryl oxidase (ERV1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. N-terminal Halo-XLF

    Plasmid

    #207546

    Purpose

    Homologous recombination donor to insert halo tag at the N terminal of the endogenous XLF locus.

    Depositor

    Jens Schmidt

    Article

    Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

    Insert

    HaloTag with internal PuroR cassette flanked by human Lig4 locus sequences
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO.1 RagA shRNA #1

    Plasmid

    #30319

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    RagA shRNA (RRAGA Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pRRL WS1.6 WASp

    Plasmid

    #36250

    Depositor

    David Rawlings

    Article

    Astrakhan et al Blood. 2012 Mar 19.

    Insert

    WASp (WAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    WS1.6

    Available since

    July 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. lentiMutB3GALT5-blast

    Plasmid

    #208381

    Purpose

    lentiviral vector for expressing human B3GALT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

    Depositor

    Jennifer Kohler

    Insert

    beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
    

    Use

    Lentiviral

    Tags

    myc, FLAG

    Expression

    Mammalian

    Mutation

    nucleotide change in PAM targeting sequence nt 51…

    Promoter

    EF-1 alpha core promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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