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We narrowed to 75,864 results for: Rest

Showing: 8751 - 8800 of 75864 results

  1. pOpen-MMLV_RT (mut H)

    Plasmid
    #165546
    Purpose
    Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165546-plasmid-map-sequence-id-339423

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  2. Pet28a_Human_H2B.1

    Plasmid
    #42630
    Depositor
    Joe Landry
    Insert
    Human H2B.1 (H2BC8 Human)
    Use
    Tags
    Untagged
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 22, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    42630-plasmid-map-sequence-id-57745

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  3. pDual_dsCas9_Blast

    Plasmid
    #214681
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two empty sgRNA cloning sites
    Depositor
    Kevin Janes
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214681-plasmid-map-sequence-id-428811

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  4. eGFP-ova-C1

    Plasmid
    #163524
    Purpose
    Expresses both eGFP and chicken ovalbumin in mammalian cells
    Depositor
    Thomas Weber
    Insert
    enhanced-GFP-chicken ovalbumin tandem
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    163524-plasmid-map-sequence-id-321577

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  5. pet30b-7M SrtA

    Plasmid
    #51141
    Purpose
    expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity
    Depositor
    Hidde Ploegh
    Insert
    S.aureus SrtA 7M
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    P94R, E105K, E108Q, D160N, D165A, K190E, K196T
    Promoter
    T7
    Available since
    Feb. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    51141-plasmid-map-sequence-id-211598

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  6. pSpCas9(BB)-2A-Neo

    Plasmid
    #127762
    Purpose
    Used for expression of sgRNA in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127762-plasmid-map-sequence-id-247554

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  7. pPD1101 Integration Test Circuit Vector

    Plasmid
    #139251
    Purpose
    Integration vector with TUs incorporated. Cells will be red and, if treated with doxycycline, blue.
    Depositor
    Joshua Leonard
    Insert
    Tus
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    139251-plasmid-map-sequence-id-337940

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  9. pOpen-lambda red operon

    Plasmid
    #165521
    Purpose
    Operon containing Exo, Bet, and Gam. To use the lambda red recombineering system to modify your target DNA.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    lambda red operon
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 3, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165521-plasmid-map-sequence-id-338838

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  10. pAAV-EF1a-DIO-mCherry

    Plasmid
    #50462
    Purpose
    Double floxed mCherry under the control of EF1a promoter
    Depositor
    Bryan Roth
    Insert
    mCherry
    Use
    AAV
    Tags
    N/A
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    March 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    50462-plasmid-map-sequence-id-182309

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  11. pRSET.HaloTag.v7.AviTag

    Plasmid
    #195486
    Purpose
    Bacterial expression plasmid with Halotag v7 insert plus a C-terminus appended AviTag for enzymatic biotinylation.
    Depositor
    HHMI-JRC Tool Translation Team, Tim Brown
    Insert
    HaloTag.v7.AviTag
    Use
    Tags
    6xHIS and AviTag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    195486-plasmid-map-sequence-id-393193

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  12. pLKO5d.SFFV.Cas9.P2A.BSD

    Plasmid
    #181981
    Purpose
    Lentiviral Cas9 expression
    Depositor
    Jan-Henning Klusmann
    Insert
    Cas9 (cas9 Streptococcus pyogenes (strain: NGAS638))
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    181981-plasmid-map-sequence-id-366611

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  13. pAAV-Ef1a-DIO EYFP

    Plasmid
    #27056
    Depositor
    Karl Deisseroth
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, and AAV9
    Insert
    EYFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2011
    Availability
    Academic Institutions and Nonprofits only
    27056-plasmid-map-sequence-id-126562

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  14. MIGR-mFoxP3

    Plasmid
    #24067
    Depositor
    Dan Littman
    Insert
    Foxp3 (Foxp3 Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 27, 2010
    Availability
    Academic Institutions and Nonprofits only
    24067-plasmid-map-sequence-id-473505

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  15. CMV-mt4xi-GCaMP6f

    Plasmid
    #192075
    Purpose
    Fluorescent reporter for mitochondrial calcium
    Depositor
    Timothy Ryan
    Insert
    4 repeats of improved COX8 signal peptide plus GCaMP6f
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    192075-plasmid-map-sequence-id-378919

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  17. pcDNA3-TRAM-CFP

    Plasmid
    #13027
    Depositor
    Doug Golenbock
    Insert
    Toll-like Receptor Adaptor Molecule (TICAM2 Human)
    Use
    Tags
    CFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13027-plasmid-map-sequence-id-7425

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  18. Rich-pT7

    Plasmid
    #108445
    Purpose
    This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pUC57. Xenopus globin 3UTR and 5UTR, pT7, and polyA added.
    Depositor
    Richard Bélanger
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mutation
    Promoter
    pT7
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    108445-plasmid-map-sequence-id-217180

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  19. pAAV-hSyn-EGFP-KASH

    Plasmid
    #209201
    Purpose
    Neuronal expression of EGFP tagged KASH protein domain
    Depositor
    Larry Zweifel
    Insert
    eGFP-KASH
    Use
    AAV
    Tags
    KASH localization domain
    Expression
    Mammalian
    Mutation
    Promoter
    hsyn
    Available since
    March 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    209201-plasmid-map-sequence-id-418201

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  20. pLenti CMVtight eGFP Neo (w784-1)

    Plasmid
    #26586
    Purpose
    2nd generation lentiviral vector
    Depositor
    Eric Campeau
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Lentiviral; Tet-on advanced vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 6, 2011
    Availability
    Academic Institutions and Nonprofits only
    26586-plasmid-map-sequence-id-12596

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  21. pET His6 NusA TEV LIC cloning vector (1N)

    Plasmid
    #29657
    Depositor
    Scott Gradia
    Type
    Empty backbone
    Use
    Tags
    6xHis, NusA, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    Aug. 17, 2011
    Availability
    Academic Institutions and Nonprofits only
    29657-plasmid-map-sequence-id-418922

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  22. pT2-3X-FLAG-H3.1-K27M-BFP

    Plasmid
    #209439
    Purpose
    SB-transposable plasmid expressing a FLAGGED mutant histone H3.1-K27M with BFP
    Depositor
    Maria Castro
    Insert
    H3.1-K27M
    Use
    Tags
    Expression
    Mammalian
    Mutation
    K27M
    Promoter
    Available since
    March 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    209439-plasmid-map-sequence-id-424832

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  23. pLV-mScarlet-I

    Plasmid
    #159172
    Purpose
    To express a bright monomeric red FP for labelling and detection of mammalian cells
    Depositor
    Pantelis Tsoulfas
    Insert
    mScarlet-I
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Codons were optimized to Human codon usage
    Promoter
    CMV
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159172-plasmid-map-sequence-id-311154

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  24. CYP2C19

    Plasmid
    #69606
    Purpose
    For protein expression of CYP2C19
    Depositor
    Joyce Goldstein
    Insert
    CYP2C19 (CYP2C19 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    deletion of AA1-8 and mutation I331V
    Promoter
    Available since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    69606-plasmid-map-sequence-id-193146

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  25. pJK645

    Plasmid
    #71700
    Purpose
    Produces E. coli thioredoxin (EcTrxA)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71700-plasmid-map-sequence-id-131737

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  26. pAG GST EGFP

    Plasmid
    #236736
    Purpose
    Bacterial expression plasmid containing GST-tagged EGFP
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    EGFP
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    Tac
    Available since
    April 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236736-plasmid-map-sequence-id-469209

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  27. pLVX-H3.3K27M-FLAG-BFP

    Plasmid
    #209434
    Purpose
    Lentiviral packagable plasmid expressing FLAGGED human histone mutant H3.3-K27M with BFP
    Depositor
    Maria Castro
    Insert
    H3.3-K27M
    Use
    Tags
    Expression
    Mammalian
    Mutation
    K27M
    Promoter
    Available since
    March 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    209434-plasmid-map-sequence-id-424833

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  28. SspB-iRFP-DHPH1-TRIO

    Plasmid
    #176119
    Purpose
    Heterodimerization with iLID, Rac1 and RhoG activation
    Depositor
    Dorus Gadella
    Insert
    TRIO (TRIO Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    176119-plasmid-map-sequence-id-346321

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  29. pMCMV3

    Plasmid
    #85711
    Purpose
    Expression vector employing the mouse CMV major immediate-early promoter enhancer (MIEP); there is a small polylinker (PstI, SmaI, XbaI, SalI ,HpaI) followed by the SV40 polyA signal sequences
    Depositor
    Ulrich Koszinowski, Martin Messerle
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    MIEPmouse CMV MIEP
    Available since
    April 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    85711-plasmid-map-sequence-id-177907

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  30. NADK

    Plasmid
    #28268
    Depositor
    Cheryl Arrowsmith
    Insert
    NADK:HPC0AC-F12:C216828 (NADK Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    contains residues 68-426.
    Promoter
    Available since
    Feb. 10, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  31. pREP-NFAT2

    Plasmid
    #11788
    Depositor
    Anjana Rao
    Insert
    NFAT2 (NFATC1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 25, 2006
    Availability
    Academic Institutions and Nonprofits only
    11788-plasmid-map-sequence-id-323906

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  32. pSpot-Tag-GFP-GPI

    Plasmid
    #117061
    Purpose
    For mammalian expression of GPI-anchored GFP with N-terminal Spot-Tag
    Depositor
    Felix Hartlepp
    Insert
    GPI-anchored GFP
    Use
    Tags
    GPI-anchor and Spot-Tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    117061-plasmid-map-sequence-id-224390

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  33. pOpen-K12polLF (exo-)

    Plasmid
    #165522
    Purpose
    DNA pol fragment used in flourescent labelling for microarray, dA and dT tailing, and ligating DNA adapters to DNA fragments.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    DNA Polymerase I, Large (Klenow) Fragment (3'-5' exo-)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165522-plasmid-map-sequence-id-350938

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  35. SGEP-sh-Hs-PHGDH-1956

    Plasmid
    #188670
    Purpose
    shRNA
    Depositor
    Lewis Cantley
    Insert
    shPHGDH (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188670-plasmid-map-sequence-id-375063

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  36. SGEP-sh-Hs-PHGDH-1883

    Plasmid
    #188669
    Purpose
    shRNA
    Depositor
    Lewis Cantley
    Insert
    shPHGDH (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188669-plasmid-map-sequence-id-375055

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  37. P22_wtCP_SP141his

    Plasmid
    #122651
    Purpose
    Expression of P22 virus like particle in bacteria
    Depositor
    Trevor Douglas
    Insert
    P22 Coat: WT / SP: 6xHis-SP141
    Use
    Tags
    Expression
    Bacterial
    Mutation
    truncated SP, P120H in the capsid scaffold (see …
    Promoter
    Available since
    Aug. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    122651-plasmid-map-sequence-id-237561

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  38. pENTR11 CA NFAT1

    Plasmid
    #11792
    Depositor
    Anjana Rao
    Insert
    NFAT1 (Nfatc2 Mouse)
    Use
    Tags
    HA
    Expression
    Bacterial
    Mutation
    Constitutively active: multiple serine to alanine…
    Promoter
    Available since
    Aug. 7, 2006
    Availability
    Academic Institutions and Nonprofits only
    11792-plasmid-map-sequence-id-229776

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  40. pLNCX chick v-src

    Plasmid
    #14578
    Depositor
    Joan Brugge
    Insert
    v-Src (SRC Chicken)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 12, 2007
    Availability
    Academic Institutions and Nonprofits only

  42. XLone-PE2

    Plasmid
    #136463
    Purpose
    Expresses prime editing 2 (PE2) enzyme upon doxycycline treatment
    Depositor
    Xiaojun Lian
    Insert
    PE2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3GS
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    136463-plasmid-map-sequence-id-272540

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  43. pGL3-Basic-Pax8

    Plasmid
    #11994
    Depositor
    Steven Johnson
    Insert
    PAX8 (PAX8 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    The following construct contains the PAX8 promote…
    Promoter
    Available since
    July 25, 2007
    Availability
    Academic Institutions and Nonprofits only
    11994-plasmid-map-sequence-id-266089

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  44. pUC119-MCS

    Plasmid
    #58807
    Purpose
    contains multiple cloning sites (MCSs) for cloning and transient expression of single or multiple guide RNA (gRNA) expression cassettes.
    Depositor
    Jen Sheen
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    58807-plasmid-map-sequence-id-91105

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  47. pCer-C3-Plk1

    Plasmid
    #68132
    Purpose
    Expression of Cerulean-tagged Plk1 in mammalian cells
    Depositor
    Catherine Lindon
    Insert
    Polo-like kinase 1 (PLK1 Human)
    Use
    Tags
    Cerulean
    Expression
    Mammalian
    Mutation
    wild-type
    Promoter
    CMV
    Available since
    Oct. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    68132-plasmid-map-sequence-id-123472

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  48. pmR-mCherry miR-31

    Plasmid
    #84916
    Purpose
    mammalian expression of miR-31
    Depositor
    Keith Baar
    Insert
    miR-31 (Mir31 Mouse)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 14, 2019
    Availability
    Academic Institutions and Nonprofits only

  49. pShuttle-CMV-F2A-T2A

    Plasmid
    #62622
    Purpose
    AdEasy Shuttle vector modified for multicistronic expression. Contains T2A and F2A
    Depositor
    Zheng-Xu Wang
    Insert
    MCS with F2A and T2A
    Use
    Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62622-plasmid-map-sequence-id-102955

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Showing: 8751 - 8800 of 75864 results