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We narrowed to 6,501 results for: nls

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Showing: 3501 - 3520 of 6501 results

  2. pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

    Plasmid
    #84244
    Purpose
    Expresses GA-inducible VPR-Sa dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GAI-tagBFP-Sa dCas9
    GID1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …
    Expression
    Mammalian
    Mutation
    D10A, N580A and Synonymous mutation in PYL1 to re…
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    84244-plasmid-map-sequence-id-160887

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  3. SpCas9-YE1-BE4max

    Plasmid
    #198546
    Purpose
    Expresses human codon-optimized SpCas9-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-YE1-BE4max-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-YE1-BE4max
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198546-plasmid-map-sequence-id-390295

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  4. SpCas9-NG-SsAPOBEC3B

    Plasmid
    #198536
    Purpose
    Expresses human codon-optimized SpCas9-NG-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NG-SsAPOBEC3B-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-SsAPOBEC3B
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198536-plasmid-map-sequence-id-388885

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  5. VanGlow-GL

    Plasmid
    #83342
    Purpose
    for Gateway cloning of promoter elements upstream of a GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.
    Depositor
    Cheng-Yu Lee
    Publication
    Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    GFP::Luciferase(nls)
    Expression
    Insect
    Available since
    Dec. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    83342-plasmid-map-sequence-id-158671

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  8. pUC57-ITR2-H1- sgCTG Bilbo -eCMV-CjSpD8A-3XFLAG-SV40 NLS-ITR2

    Plasmid
    #210749
    Purpose
    Coding for CjSp D8A alongside Bilbo sgRNA targeting CAG repeats
    Depositor
    Vincent Dion
    Publication
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    CjSp D8A Cas9
    Bilbo sgCAG
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    D8A, N-terminal Cj Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    210749-plasmid-map-sequence-id-417988

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  9. VPR-ps-dSpCas9

    Plasmid
    #102855
    Purpose
    A single-chain light-controllable dSpCas9 with pdDronpa1.2 domains
    Depositor
    Michael Lin
    Publication
    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dSpCas9, pdDronpa1.2
    Use
    CRISPR and Synthetic Biology
    Tags
    NLS and VP64-p64-Rta transcription activator
    Expression
    Mammalian
    Promoter
    PGK
    Available since
    Nov. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    102855-plasmid-map-sequence-id-199006

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  10. 3xFLAG-dCas9/pMXs-IG

    Plasmid
    #51258
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Publication
    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    51258-plasmid-map-sequence-id-77869

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  11. pBridge-tyrosinase.σ2

    Plasmid
    #197415
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Publication
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-2 σ2
    Tags
    GAL4-DNA binding domain fragment, HA tag, and nuc…
    Expression
    Yeast
    Mutation
    encodes R42G substitution, contains silent substi…
    Promoter
    ADH1 and MET25
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197415-plasmid-map-sequence-id-388893

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  13. pBUN6I11

    Plasmid
    #50580
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9-KRAB
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Mutation
    dCas9 that is defective in DNA cleavage; the maiz…
    Promoter
    OsU3p and Ubi1p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    50580-plasmid-map-sequence-id-75637

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  14. pSL1180-HR-PUbECFP

    Plasmid
    #47917
    Purpose
    Donor plasmid for homologous recombination that expresses ECFP consitutively using the Aedes aegypti polyubiquitin (PUb) promoter.
    Depositor
    Leslie Vosshall
    Insert
    ECFP
    Tags
    NLS
    Expression
    Insect
    Promoter
    Aedes aegypti polyubiquitin (PUb)
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    12 Articles
    47917-plasmid-map-sequence-id-68604

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  19. pYPQ167

    Plasmid
    #69309
    Purpose
    Gateway entry vector with Cas9p
    Depositor
    Yiping Qi
    Publication
    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9p (Plant codon-optimized; high GC content at 5 prime region)
    Use
    CRISPR
    Mutation
    NLS fusion
    Available since
    Sept. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    69309-plasmid-map-sequence-id-123952

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  20. pTE4497

    Plasmid
    #80339
    Purpose
    mammalian expression FnCpf1 nuclease and Fn crRNA
    Depositor
    Ervin Welker
    Publication
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Fn crRNA
    FnCpf1
    Use
    CRISPR
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Promoter
    CMV and human U6
    Available since
    Aug. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    80339-plasmid-map-sequence-id-151162

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Showing: 3501 - 3520 of 6501 results