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pARS3_MUbCAS9_GW

Plasmid

#128646

Purpose

T-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a Gateway cassette for cloning gRNA

Depositor

G. Eric Schaller

Article

Worthen et al Development. 2019 Jun 3. pii: dev.174870

Type

Empty backbone

Tags

HA

Expression

Plant

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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myc-hULK2

Plasmid

#31966

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

ULK2 (ULK2 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only
(R42A) Cyclin B1-GFP (62)

Plasmid

#61849

Purpose

mammalian expression of Cyclin B1 with R42A mutation in destruction box to make cyclin B1 non-destructable

Depositor

Jonathon Pines

Article

Clute et al Nat Cell Biol. 1999 Jun;1(2):82-7.

Insert

cyclin B1 (CCNB1 Human)

Tags

GFP and Myc

Expression

Mammalian

Mutation

V5A R42A

Promoter

CMV

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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TRPML1-CFP

Plasmid

#18827

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML1 (MCOLN1 Human)

Tags

CFP

Expression

Mammalian

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
Erk2

Plasmid

#29582

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

Erk2 (Mapk1 Rat)

Tags

HIS6 and TEV

Expression

Bacterial

Mutation

Starting Met removed from Erk2

Available Since

July 16, 2011

Availability

Academic Institutions and Nonprofits only

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pLKO.1 RagC shRNA #1

Plasmid

#26629

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RagC shRNA (RRAGC Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-LplA(W37V) Coumarin PRIME ligase

Plasmid

#25845

Depositor

Alice Ting

Article

Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

Insert

LplA-W37V

Tags

Flag and His

Expression

Mammalian

Mutation

W37V

Available Since

July 27, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV10-3xFLAG-prs-XK

Plasmid

#184369

Purpose

Express human XK in Expi293 cells

Depositor

Karin Reinisch

Article

Adlakha et al J Cell Biol. 2022 Jun 6;221(5):e20220203

Insert

Human XK (XK Human)

Tags

3xFLAG-PreScission cleavage site

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pR023_PIE-PUM2

Plasmid

#204819

Purpose

Expression PIE-PUM2 fusion protein in mammalian cells

Depositor

Xiaochang Zhang

Article

Ruan et al Nat Commun. 2023 Jun 6;14(1):3275. doi:

Insert

Pumilio 2 (PUM2 Human)

Tags

HA-Flag-rApobec1 and huADAR2dd

Expression

Mammalian

Promoter

chicken β-actin promoter

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pPW3781 : CMVd3-ERmembrane-GST-HaloTag-KKMP

Plasmid

#185686

Purpose

Low-level transient expression of a HaloTag protein targeted to the ER membrane, fused with a GST for constitutive dimerization.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-GST-HaloTag-KKMP

Expression

Mammalian

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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cr3Dd

Plasmid

#184862

Purpose

cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and a species-specific recombination system

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

Cas9, bet, exo

Tags

Cas9 ssrA degradation tag

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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hRIP3 GFP D160N

Plasmid

#41386

Depositor

Francis Chan

Article

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP3 (RIPK3 Human)

Tags

GFP

Expression

Mammalian

Mutation

Kinase Domain Mutant (D160N)

Promoter

CMV

Available Since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only

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Flag-p50(1-435)

Plasmid

#44747

Depositor

Gourisankar Ghosh

Article

Savinova et al Mol Cell. 2009 Jun 12;34(5):591-602. doi

Insert

p50 (Nfkb1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

AA 1-435

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pTlpA_mCherry_Txe/Axe

Plasmid

#229147

Purpose

Toxin-Antitoxin (Txe/Axe)

Depositor

Shrikrishnan Sankaran

Article

Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

Insert

mCherry, Txe/Axe

Expression

Bacterial

Promoter

PtlpA (mCherry), Txe/Axe (Native)

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+ cas

Plasmid

#26729

Depositor

Didier Stainier

Article

Kikuchi et al Genes Dev. 2001 Jun 15. 15(12):1493-505.

Insert

casanova (sox32 Zebrafish)

Use

Xenopus/mammalian/avian/zebrafish

Available Since

Jan. 18, 2011

Availability

Academic Institutions and Nonprofits only
mCherry-PIK3R1

Plasmid

#174192

Purpose

Expresses PIK3R1 in mammalian cells

Depositor

Cheng-han Yu

Article

Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

Insert

PIK3R1 (PIK3R1 Human)

Tags

mCherry

Expression

Mammalian

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1 RagD shRNA #1

Plasmid

#30321

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RagD shRNA (RRAGD Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 2, 2011

Availability

Academic Institutions and Nonprofits only
pME-PLIN2-tdTOMATO

Plasmid

#172533

Purpose

PLIN2-tdTOMATO fusion (zebrafish lipid droplet reporter) in middle entry vector for Gateway cloning

Depositor

Richard White

Article

Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

Insert

plin2-tdtomato

Mutation

S80R, A81S, E144D, I148F, and R415H mutations in …

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pmNFH

Plasmid

#85178

Purpose

Mouse neurofilament protein H cDNA mammalian expression construct

Depositor

Anthony Brown

Article

Colakoglu et al J Cell Biol. 2009 Jun 1;185(5):769-77. d

Insert

mouse NFH (Nefh Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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ATM-Halo HRD

Plasmid

#207082

Purpose

Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous ATM locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

HaloTag followed by a PolyA signal and PuroR cassette flanked by human ATM locus sequences

Tags

HaloTag

Expression

Mammalian

Promoter

None

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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