We narrowed to 4,189 results for: Pet2

1. pBEL1751

   Plasmid

   #139876

   Purpose

   Expression plasmid for naturally occurring 4xMCE protein (without TM region)

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   Desulfocapsa_sulfexigens_UWK_02604
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Desulfocapsa_sulfexigens_UWK_02604(38-533)-6xHis

   Promoter

   Available since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDC24

   Plasmid

   #131368

   Purpose

   expression of MEG-3Cterm (aa545-862) in C. elegans

   Depositor

   Geraldine Seydoux

   Article

   Smith et al Elife. 2016 Dec 3;5. pii: 21337. doi: 10

   Insert

   meg-3 (aa545-862) (meg-3 Nematode)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDC21

   Plasmid

   #131367

   Purpose

   expression of MEG-3IDR (aa1-544) in C. elegans

   Depositor

   Geraldine Seydoux

   Article

   Smith et al Elife. 2016 Dec 3;5. pii: 21337. doi: 10

   Insert

   meg-3 (aa1-544) (meg-3 Nematode)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. 6CUI (BRD2)

   Plasmid

   #119963

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   BRD2 (BRD2 Human)
   

   Use

   Tags

   GST tag followed by a long linker and TEV cleavag…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 1, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. 6DUB (LOC149281)

   Plasmid

   #119967

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   LOC149281 (METTL11B Human)
   

   Use

   Tags

   6xHIS, thrombin,SUMO and TEV cleavage

   Expression

   Bacterial

   Mutation

   contains amino acids 58-278

   Promoter

   T7

   Available since

   Jan. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. 5T1G (CBX1)

   Plasmid

   #101884

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   CBX1 (CBX1 Human)
   

   Use

   Tags

   6xHis, TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 30, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pET-PF2050

   Plasmid

   #100721

   Purpose

   Expression of Pyrococcus furiosus PF2050

   Depositor

   Byung Il Lee

   Article

   Han et al FEBS Lett. 2012 May 7;586(9):1384-8. doi

   Insert

   PF2050
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promotor

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. 2QO2 (EPHA3)

   Plasmid

   #101271

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   EPHA3 (EPHA3 Human)
   

   Use

   Tags

   Hexahistidine tag with Thrombin cleavage

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 2, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. CTL2

   Plasmid

   #92212

   Purpose

   Expresses C-Terminal segment of C. elegans Piezo1 in E. coli

   Depositor

   Douglas Rees

   Article

   Kamajaya et al Structure. 2014 Oct 7;22(10):1520-7. doi

   Insert

   CTL2 of PIEZO1
   

   Use

   Tags

   6x Histidine Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ppj219(JBEI-13475)

    Plasmid

    #89785

    Purpose

    for expression of N-StrepII-3607

    Depositor

    Harry Beller

    Article

    Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

    Insert

    3607
    

    Use

    Tags

    StrepII

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Ppj184 (JBEI-13431)

    Plasmid

    #89784

    Purpose

    for expression of N-His-3607

    Depositor

    Harry Beller

    Article

    Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

    Insert

    3607
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Ppj187 (JBEI-13437)

    Plasmid

    #89783

    Purpose

    for expression of C-His-3607

    Depositor

    Harry Beller

    Article

    Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

    Insert

    3607
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJK_proNR2_LGK.9

    Plasmid

    #81162

    Purpose

    Expresses levoglucosan kinase variant LGK.9 with promoter proNR2

    Depositor

    Timothy Whitehead

    Article

    Klesmith et al ACS Synth Biol. 2015 Sep 14.

    Insert

    Levoglucosan kinase
    

    Use

    Tags

    His6x

    Expression

    Bacterial

    Mutation

    LGK.9 - (T15R, A57Q, R94G, R100C, N101G, S105R, L…

    Promoter

    proNR2

    Available since

    Sept. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lf-MetQ

    Plasmid

    #78305

    Purpose

    Methionine Binding Protein (MetQ) with C-Terminal 6x Histidine Tag

    Depositor

    Douglas Rees

    Article

    Nguyen et al Biol Chem. 2015 Sep;396(9-10):1127-34. d

    Insert

    Methionine Binding Protein (metQ )
    

    Use

    Tags

    6x Histidine Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. His10-PS-SNAPf-9R (XSB525)

    Plasmid

    #78513

    Purpose

    Construct for expressing His10 - SNAPf -9R in E. Coli

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    SNAPf
    

    Use

    Tags

    His10 followed by a prescission protease claevage…

    Expression

    Bacterial

    Mutation

    9R added to the C-terminal of SNAPf

    Promoter

    Available since

    June 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJK040 (pET-PurQ-A128T)

    Plasmid

    #73840

    Purpose

    Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, A128T mutant (PurQ-A128T)

    Depositor

    JoAnne Stubbe, T. J. Kappock

    Article

    Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

    Insert

    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A128T mutant

    Promoter

    T7

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJK044 (pET-PurQ-wt)

    Plasmid

    #73839

    Purpose

    Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, (PurQ)

    Depositor

    JoAnne Stubbe, T. J. Kappock

    Article

    Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

    Insert

    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJK598

    Plasmid

    #72523

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with C-terminal His6 tag, and associated hypothetical protein (AaPurS, AaOrfY, AaPurQ, AaPurL)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    synthetic gene

    Promoter

    T7 and n/a

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJK560

    Plasmid

    #71585

    Purpose

    Produces E. coli acetyl-CoA:oxalate CoA-transferase (yfdE) with Arg232>His mutant (YfdE-R232H)

    Depositor

    T. J. Kappock

    Article

    Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

    Insert

    acetyl-CoA:oxalate CoA-transferase (yfdE )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed arginine-232 to histidine

    Promoter

    T7 promoter

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJK504

    Plasmid

    #71584

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase (AarC) with C-terminal His6 tag and Cys357>Tyr mutant (AarCH6-C357Y)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Acetic Acid Bacteria. 2(s1):e3 (2013)

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes cysteine-357 to tyrosine

    Promoter

    T7

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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