We narrowed to 6,447 results for: nls
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Plasmid#184714PurposeGq DREADDDepositorInserthM3D
ExpressionMammalianAvailable SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E8-dTom-nlsdTom
Plasmid#135643PurposeAAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E8 regulatory elementDepositorInsertdTom-nlsdTom
UseAAVMutationNoneAvailable SinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBB-GFP-hBEN-deltaNLS3
Plasmid#22158DepositorAvailable SinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEFBOS_CreIRESpuro
Plasmid#183812PurposeExpresses Cre recombinase and puromycin N-acetyltransferase driven by the mammalian EF1a promoterDepositorInsertCre recombinase
UseSynthetic BiologyTagsNLSExpressionMammalianAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NRCH-ABE8e(V106W)
Plasmid#198556PurposeExpresses human codon-optimized SpCas9-NRCH-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8e(V106W)-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NRCH-ABE8e(V106W)
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
VanGlow-RR
Plasmid#83343Purposefor Gateway cloning of promoter elements upstream of a mCherry::Renilla(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.DepositorTypeEmpty backboneUseLuciferaseTagsmCherry::Renilla(nls)ExpressionInsectAvailable SinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV x EF1a_EKAREN5-ires-puro
Plasmid#167821PurposeOptimized EKAREV FRET biosensor sensor for ERKDepositorInsertEKAREN5
UseLentiviralTagsnls localization motifExpressionMammalianMutationK424P, K426WPromoterEF1aAvailable SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCL1002
Plasmid#252428PurposemCherry-tagged NASP with nuclear localisation sequence (NLS) deletedDepositorInsertsNASP-dNLS (NASP Human)
TagsmCherryExpressionMammalianMutationdeletion of NLS (residues 378-383)PromoterCMVAvailable SinceMarch 10, 2026AvailabilityAcademic Institutions and Nonprofits only -
PCO4SV40_pDONR201
Plasmid#249815PurposeEncodes PCO4 fused to a SV40-based NLS, Gateway compatible as Donor plasmidDepositorAvailable SinceJan. 28, 2026AvailabilityAcademic Institutions and Nonprofits only -
pAN052
Plasmid#220046PurposeMammalian expression of type III-A Csm complex from Streptococcus thermophilus without NLS tagsDepositorInsertFLAG-Csm1; FLAG-Csm2; FLAG-Csm3; FLAG-Csm4; FLAG-Csm5; FLAG-Cas6; crRNA; GFP
ExpressionMammalianAvailable SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Nuc-ABKAR
Plasmid#61511PurposeExpression of nuclear AMPK biosensorDepositorInsertAMPK Biosensor
TagsNLS (see comments)ExpressionMammalianPromoterCMVAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
plko_TRE3G_nlsMyc-Sox9HMGtruncation_PGKpuroR
Plasmid#203964PurposeDox inducible myc tag truncated SOX9 without DNA-binding domain (noHMG)DepositorInsertSOX9-noHMG (Sox9 Mouse)
UseLentiviralTagsmycMutationthe HMG domain is truncatedPromoterTRE3GAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
mutNLS V5-Twist
Plasmid#201380PurposeExpresses Twist mutant for nucleocytoplasmic transport studiesDepositorAvailable SinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
bpNLS-DddIA
Plasmid#187847Purposeexpresses the bpNLS-linked DddIA in mammalian cellsDepositorInsertbpNLS-DddIA-bpNLS-p2A-EGFP
ExpressionMammalianAvailable SinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCC_11 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9-NLS-2A-Puro-WPRE
Plasmid#139096PurposeExpresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.DepositorInsertKRAB-dxCas9 3.7
UseCRISPR and LentiviralExpressionMammalianMutationD10A, H840A, A262T, R324L,S409I, E480K, E543D, M6…Available SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GoldenGate
Plasmid#47388PurposeExpression plasmid for TALE proteins compatible with GoldenGate cloning systemDepositorTypeEmpty backboneUseTALENTagsFlag, HA, and SV40 NLSExpressionMammalianPromoterCMVAvailable SinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HF1RA-P2A-GFP-PGK-Puro
Plasmid#110866PurposeLentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized)DepositorInsertCas9-HF1RA
UseLentiviralTagsFLAGMutationR661A, Q695A, Q926A, and NLS sequence at the N-te…PromoterEF1sAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ151
Plasmid#69302PurposeGateway entry vector with pcoCas9D10ADepositorInsertpcoCas9D10A (Plant codon-optimized)
UseCRISPRTags2XFLAGMutationD10A mutation; NLS fusionAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GoldenGate-VP64
Plasmid#47389PurposeExpression plasmid for TALE proteins fused to VP64 activation domain compatible with GoldenGate cloning systemDepositorTypeEmpty backboneUseTALENTagsFLAG, HA, and SV40 NLSExpressionMammalianPromoterCMVAvailable SinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAT253
Plasmid#40943DepositorInsertGFP-LacI**
TagsGFP(S65T, V163A) and SV40 NLSExpressionYeastMutationLacI**/LacI2G(4 mutations) (see comment below); l…PromoterHis3 promoter fragmentAvailable SinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only