We narrowed to 57,746 results for: plasmid

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pSpCas9_T2A_mCherry sgAAVS1

Plasmid

#192480

Purpose

All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1 or V2 cells as control

Depositor

Michael Yaffe

Publication

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SpCas9

Tags

T2A mCherry

Expression

Mammalian

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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PEA1-GFP

Plasmid

#171993

Purpose

Delivers all prime editing nickase components in a single, GFP selectable plasmid

Depositor

Paul Thomas

Publication

Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CbH-Cas9(H840A)-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)

Expression

Mammalian

Promoter

CMV for Cas9, U6 for gRNAs

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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4xHRE-MinTK-CRE-ODD

Plasmid

#141147

Purpose

lentiviral expression vector to generate a hypoxia fate-mapping system

Depositor

Daniele Gilkes

Publication

Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

Insert

4xHRE-MinTK-CRE-ODD

Use

Lentiviral

Mutation

contains an altered Cre gene modified by the addi…

Promoter

synthetic HIF–DNA binding sequence (HRE)

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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HA-GFP

Plasmid

#137763

Purpose

Control plasmid for immunoprecipitation of HA-tagged proteins. Also a good control for transfection efficiency.

Depositor

Carol Mercer

Publication

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

GFP

Tags

HA and myc

Expression

Mammalian

Promoter

CMV

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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tsPurple chromoprotein

Plasmid

#117848

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses tsPurple chromoprotein in E. coli

Depositor

Anthony Forster

Publication

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, tsPurple

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGTMV

Plasmid

#118755

Purpose

Binary plasmid with a tobacco mosaic virus infectious clone; Agrobacterium-mediated transformation required for expression in plants.

Depositor

Jose-Antonio Daros

Publication

Shi et al Plant Biotechnol J. 2018 Nov 22. doi: 10

Insert

Tobacco mosaic virus

Expression

Plant

Promoter

Cauliflower mosaic virus 35S

Available since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pME-MLS-KillerRed

Plasmid

#115517

Purpose

Middle entry plasmid containing membrane bound KillerRed fluorophore

Depositor

Marco Morsch

Publication

Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

Insert

MLS-KillerRed

Use

Gateway Cloning

Tags

MLS

Available since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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lenti-SpCas9 puro

Plasmid

#104994

Purpose

This 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.

Depositor

Brett Stringer

Publication

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

KpnI-XhoI-BsrGI-NheI

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKM446 (Flag-DAS+4))

Plasmid

#108321

Purpose

Orbit integrating plasmid for C-terminal tagging with degradation tag DAS+4, using hygromycin as a selection marker.

Depositor

Kenan Murphy

Publication

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

attB-Flag-DAS+4

Use

Orbit integrating plasmid

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-TagRFP657

Plasmid

#96939

Purpose

Retroviral plasmid containing TagRFP657 for labeling of cells

Depositor

Lee Grimes

Publication

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Tag Red Fluorescent Protein 657

Expression

Mammalian

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEB1-mGFPmut2

Plasmid

#103980

Purpose

Plasmid encoding mGFPmut2

Depositor

Philippe Cluzel

Publication

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mGFPmut2

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S65A, V68L, …

Promoter

proC

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223_STAT3_WT

Plasmid

#82235

Purpose

Gateway Donor vector containing STAT3, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAT3 (STAT3 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCX-HO1-2A-EGFP

Plasmid

#74672

Purpose

Mammalian expression of HO1 and EGFP

Depositor

Roberto Giovannoni

Publication

Cinti et al PLoS One. 2015 Oct 29;10(10):e0141933. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HO1 (HMOX1 Human)

Tags

2A-EGFP

Expression

Mammalian

Promoter

pCAGGS

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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CRISPseq-BFP-backbone

Plasmid

#85707

Purpose

Backbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with a BFP fluorescent marker. Does NOT include Cas9.

Depositor

Ido Amit

Publication

Jaitin et al Cell. 2016 Dec 15;167(7):1883-1896.e15.

Type

Empty backbone

Use

Lentiviral

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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MSCV-EZH2-PGK-Puro-IRES-GFP

Plasmid

#75125

Purpose

Retroviral expression plasmid encoding wild type human EZH2

Depositor

Christopher Vakoc

Publication

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

KMT6 (EZH2 Human)

Use

Retroviral

Tags

None

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcDNA3 Flag C/EBPb LAP

Plasmid

#66979

Purpose

Expression plasmid for mouse C/EBPb LAP; adds Flag tag to the N-terminus of protein

Depositor

Christopher Vakoc

Publication

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

C/EBP beta (Cebpb Mouse)

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available since

July 15, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-C3-Sec15

Plasmid

#53760

Purpose

pEGFP-C3 plasmid that expresses Sec15

Depositor

Channing Der

Publication

Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Sec15 (EXOC6 Human)

Tags

GFP

Expression

Mammalian

Available since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pLv-CMV-MyoD

Plasmid

#26808

Depositor

Jeffrey Chamberlain

Publication

Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

Insert

MyoD (Myod1 Mouse)

Use

Lentiviral

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGW-TurboID-GOI

Plasmid

#209380

Purpose

Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta

Depositor

Rosa Lozano-Durán

Publication

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Type

Empty backbone

Expression

Plant

Mutation

TurboID gene sequence C249A

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pJA257

Plasmid

#21510

Depositor

Julie Ahringer

Publication

Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

(Gly)5Ala::GFP-F64LS65T::tbb-2 3'UTR

Use

Gateway Cloning

Available since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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