We narrowed to 4,941 results for: puc

1. pGGB-V5-TurboID-T2A-C

   Plasmid

   #218896

   Purpose

   golden gate cloning

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   V5-TurboID-T2A
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pITESceIcmd

   Plasmid

   #218288

   Purpose

   Introduction of a cyanamide-inducible I-SceI expression cassette, use in combination with pITEdv1

   Depositor

   Claudia Vickers

   Article

   McDonnell et al bioRxiv 2024.03.26.586389

   Insert

   HO(-253, -1)-tSynth3

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKE011

   Plasmid

   #207912

   Purpose

   Level 0 for gRNA in C4 module (5' GCAG-3' TCAG)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU3-AarI-scaffold-term
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS018

   Plasmid

   #207911

   Purpose

   Level 0 for gRNA in C3 module (5' TTCG-3' GCAG)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU6-AarI-scaffold-term
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCS020

   Plasmid

   #207915

   Purpose

   Level 0 for gRNA in D3 module (5' TCCC-3' CTGC)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU6-AarI-scaffold-term
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCS017

   Plasmid

   #207909

   Purpose

   Level 0 for gRNA in C1 module (5' GGCT-3' AGCC)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU6-AarI-scaffold-term
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKE012

   Plasmid

   #207914

   Purpose

   Level 0 for gRNA in AD2 module (5' TGAC-3' TCCC)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU3-AarI-scaffold-term
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKE010

   Plasmid

   #207910

   Purpose

   Level 0 for gRNA in C2 module (5' AGCC-3' TTCG)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU3-AarI-scaffold-term
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCS019

   Plasmid

   #207913

   Purpose

   Level 0 for gRNA in D1 module (5' TCAG-3' TGAC)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU6-AarI-scaffold-term
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. NK809

    Plasmid

    #196406

    Purpose

    Strong expression of firefly and nanoluc luciferases codon-optimized for S. rosetta

    Depositor

    Nicole King

    Article

    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

    Insert

    Sro5EFL-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    N/A (synthetic)

    Promoter

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. NK810

    Plasmid

    #196407

    Purpose

    Nanoluc luciferase under S. rosetta SPAG6 promoter with internal actin-firefly control

    Depositor

    Nicole King

    Article

    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

    Insert

    Sro5spag6-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    N/A (synthetic)

    Promoter

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. NK811

    Plasmid

    #196408

    Purpose

    Nanoluc luciferase under S. rosetta SPAG6 promoter with mutated RFX binding site with internal actin-firefly control

    Depositor

    Nicole King

    Article

    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

    Insert

    Sro5spag6_mut-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    N/A (synthetic)

    Promoter

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAG-PlmCasX(T26R/K610R/K808R)-T2A-EGFP

    Plasmid

    #188274

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Chen et al Innovation (Camb). 2022 May 26;3(4):1002

    Insert

    PlmCasX(T26R/K610R/K808R)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. p5’UTR-ORF1

    Plasmid

    #190566

    Purpose

    Holding vector containing the contiguous 5’UTR and ORF1 regions of human L1 (L1.3) to facilitate subcloning of ORF1 mutants into the full-length L1 reporter pRTC2-puro (Cook et al., 2015).

    Depositor

    Anthony Furano

    Article

    Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Contiguous regions of the human L1 (L1.3) 5’UTR and ORF1
    

    Use

    For dna propagation only

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    N/A

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCA23-sgRNA vector-U6-sgTS2 (SpCas9)

    Plasmid

    #199454

    Purpose

    U6-driven sgRNA targeting TS2 sequence (GGAGCTTACTGAGACTCTTC)

    Depositor

    Baohui Chen

    Article

    Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

    Insert

    TS2 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    mouse U6

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCA28-pMa-PspCas13b crRNA-TS1

    Plasmid

    #199459

    Purpose

    U6-driven crRNA targeting TS1 sequence (CCTCCTCGGAGAGCATCGGTGC )

    Depositor

    Baohui Chen

    Article

    Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

    Insert

    TS1 crRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    mouse U6

    Available since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCA11-pMa-sgTS3-17xMS2V5-sgTS3

    Plasmid

    #199442

    Purpose

    CRISPR donor plasmid to insert 17XMS2V5 into the ribosomal DNA region in dependent of homology arms

    Depositor

    Baohui Chen

    Article

    Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

    Insert

    HITI donor template
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSK-mU6-Ccu-sgRNA-SV40-puro

    Plasmid

    #192130

    Purpose

    Expresses Ccu-SgRNA scaffold cloned with BbsI and puromycin resistance gene

    Depositor

    Yongming Wang

    Article

    Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

    Insert

    Ccu-SgRNA scaffold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSK-mU6-Hsp1-sgRNA-SV40-puro

    Plasmid

    #192129

    Purpose

    Expresses Hsp1-SgRNA scaffold cloned with BbsI and puromycin resistance gene

    Depositor

    Yongming Wang

    Article

    Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

    Insert

    Hsp1-SgRNA scaffold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pILGFP4D42

    Plasmid

    #185840

    Purpose

    Testing the TEF1 promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PTEF1+[Z268]>yEGFP>TPGK1-TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge