We narrowed to 17,703 results for: puromycin

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non-targeting control gRNA (BRDN0001487130)

Plasmid

#80262

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Lazarus Gata 2 flag

Plasmid

#80137

Purpose

retroviral expression of Gata2-Flag

Depositor

Ellen Rothenberg

Insert

Gata2 (Gata2 Mouse)

Use

Retroviral

Tags

FLAG and IRES-EGFP

Expression

Mammalian

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pLJM1-EGFP-BarcodeV1

Plasmid

#78633

Purpose

GESTALT V1 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Publication

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT target V1

Use

Lentiviral

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pMIR-21 Reporter

Plasmid

#71870

Purpose

Mammalian expression vector for the analysis of miR-21 activity

Depositor

Mien-Chie Hung

Publication

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

Reverse complementary sequence of miR-21

Tags

firefly luciferase

Expression

Mammalian

Promoter

CMV

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pCDH-PGK-Nluc-P2A-copGFP-T2A-Puro

Plasmid

#73040

Purpose

Express Nluc and copGFP dual reporter in mammalian cells

Depositor

Kazuhiro Oka

Insert

Nluc-P2A-copGFP-T2A-Puro

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

PGK (phosphoglycerate kinase 1)

Available since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only

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AR-V7-pcw107-V5

Plasmid

#64636

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

AR (transcript variant 1, splice isoform) (AR Human)

Use

Lentiviral

Tags

V5

Mutation

splice isoform *

Promoter

PGK

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
Flag_HsIRE1a_deltaP29_D408_pBabePuro

Plasmid

#58422

Purpose

retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408

Depositor

David Ron

Publication

Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

Insert

IRE1a (ERN1 Human)

Use

Retroviral

Tags

FLAG and preprotrypsin signal sequence

Expression

Mammalian

Mutation

Deleted amino acids P29 to D408

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV PIG Fli1

Plasmid

#66983

Purpose

Retroviral expression plasmid for mouse Fli1 expression; containing bicistronic IRES-GFP

Depositor

Christopher Vakoc

Publication

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

Fli1 (Fli1 Mouse)

Use

Retroviral

Promoter

LTR

Available since

July 15, 2015

Availability

Academic Institutions and Nonprofits only

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pIRES Centrin1 Cherry Puro

Plasmid

#64340

Purpose

Bicistronic expression of Centrin-mCherry fusion and Puro resistance gene

Depositor

Matthieu Piel

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Centrin1

Tags

IRES-Puro and mCherry

Expression

Mammalian

Promoter

CMV

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only
pERB219

Plasmid

#61501

Purpose

Const. Halotag-GFP-PACT + mCherry-DHFR

Depositor

David Chenoweth, Michael Lampson

Publication

Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

HaloTag-GFP-PACT
mCherry-DHFR

Tags

HaloTag and mCherry

Expression

Mammalian

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pLentiX2-PURO-shLKB1-Ms

Plasmid

#61231

Purpose

Lentivirus expressing shRNA to mouse LKB1

Depositor

Reuben Shaw

Publication

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

shLkb1 (Ms/Hu) (Stk11 Mouse, Human)

Use

Lentiviral

Promoter

H1/TO

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pHAGE- TRE-DEST-NBioTAP

Plasmid

#53568

Purpose

Lentiviral destination vector for N -Bio-1xproteinA constructs using Gateway recombination System. Design for cDNA clones from the CCSBHuman Orfeome Collection.

Depositor

Mitzi Kuroda

Publication

Alekseyenko et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

Type

Empty backbone

Use

Lentiviral

Available since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLPCX-PECAMTL

Plasmid

#45851

Depositor

Martin Schwartz

Publication

Conway et al Curr Biol. 2013 May 14. pii: S0960-9822(

Insert

PECAM1 (PECAM1 Human)

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pLX302 IL10RA-V5 puro

Plasmid

#47552

Purpose

Lentiviral expression of V5-tagged IL10RA in mammalian cells

Depositor

Kevin Janes

Publication

Kang et al Sci Signal. 2013 Aug 6;6(287):rs13. doi:

Insert

IL10RA (IL10RA Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
pMRXIP GFP-Vti1b

Plasmid

#45922

Depositor

Noboru Mizushima

Publication

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

vesicle transport through interaction with t-SNAREs 1B (VTI1B Human)

Use

Retroviral

Tags

GFP

Promoter

CMV

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
pBABE puro Brca1 S1423A S1524A HA

Plasmid

#41968

Depositor

Stephen Elledge

Publication

Cortez et al Science. 1999 Nov 5. 286(5442):1162-6.

Insert

BRCA1 (BRCA1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

S1423A, S1524A

Available since

April 26, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
MSCV N-term HA-FLAG puro BRD7

Plasmid

#41934

Depositor

Stephen Elledge

Publication

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BRD7 (BRD7 Human)

Use

Retroviral

Tags

HA-Flag

Expression

Mammalian

Available since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
pLKO-shTRAF2#2

Plasmid

#44128

Depositor

William Hahn

Publication

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 (TRAF2 Human)

Use

Lentiviral and RNAi

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
pLKO.1-sh-mSlug-4

Plasmid

#40648

Depositor

Bob Weinberg

Publication

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-mSlug-4 (Snai2 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pMXs-puro GFP-b5 ER

Plasmid

#38274

Depositor

Noboru Mizushima

Publication

Itakura et al Autophagy. 2010 Aug;6(6):764-76.

Insert

Cytochrome b5 (Cyb5a Rat)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Cyb5a Tranmembrane region from 95 to 134 amino ac…

Available since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article