Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 12,157 results for: pcdna

Showing: 5311 - 5340 of 12157 results

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pcDNA3puro-EGFP-GAPDH-Tudor

Plasmid

#225479

Purpose

Express mEGFP-GAPDH-Tudor fusion

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

GAPDH fused with Tudor domain of TDRD3 (GAPDH Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-EGFP-GAPDH-RGG

Plasmid

#225477

Purpose

Express mEGFP-GAPDH-RGG fusion

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

GAPDH fused with RGG domain of TOP3B (GAPDH Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Myc-BirA*-CAMK2D

Plasmid

#221686

Purpose

Mammalian expression of Myc-tagged BirA*-CAMK2D fusion protein for BioID

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

CAMK2D (CAMK2D Human)

Expression

Mammalian

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EGFP-RalA(G23C)

Plasmid

#224275

Purpose

cell transfection and expreswsion of RalA (G23C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

RalA (RALA Human)

Expression

Mammalian

Mutation

G23C

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-FLAG-msMMS22L

Plasmid

#221015

Purpose

mammalian expression vector of Flag tagged mouse MMS22L

Depositor

Daniel Durocher

Article

O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

Insert

MMS22L (Mms22l Mouse)

Tags

Flag

Expression

Mammalian

Mutation

naturally siResistant to siMMS22L (human) aagacuu…

Promoter

CMV

Available Since

Sept. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/T0-EGFP-GNB1L

Plasmid

#222621

Purpose

Mammalian expression vector of flag-tagged GNB1L

Depositor

Daniel Durocher

Article

Zhao et al Mol Cell. 2023 Aug 3;83(15):2792-2809.e9

Insert

GNB1L (GNB1L Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA4.1-Myc-RNF8-T198A

Plasmid

#221680

Purpose

Mammalian expression of Myc-tagged human RNF8 (T198A)

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

RNF8 (RNF8 Human)

Expression

Mammalian

Mutation

T198A

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA4.1-Myc-RNF8-S157A

Plasmid

#221679

Purpose

Mammalian expression of Myc-tagged human RNF8 (S157A)

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

RNF8 (RNF8 Human)

Expression

Mammalian

Mutation

S157A

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA4.1-Myc-RNF8-C403S

Plasmid

#221678

Purpose

Mammalian expression of Myc-tagged human RNF8 (C403S)

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

RNF8 (RNF8 Human)

Expression

Mammalian

Mutation

C403S

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA4.1-Myc-RNF8-R42A

Plasmid

#221677

Purpose

Mammalian expression of Myc-tagged human RNF8 (R42A)

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

RNF8 (RNF8 Human)

Expression

Mammalian

Mutation

R42A

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Myc-BirA*-RNF8

Plasmid

#221683

Purpose

Mammalian expression of Myc-tagged BirA*-RNF8 fusion protein for BioID

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

RNF8 (RNF8 Human)

Expression

Mammalian

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-TRBP-V336A/H338A

Plasmid

#215891

Purpose

Expression of TRBP-V336A/H338A

Depositor

Kumiko Ui-Tei

Article

Komori et al Biol Open. 2020 Feb 25;9(2):bio050435. d

Insert

TRBP-V336A/H338A (TARBP2 Human)

Expression

Mammalian

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/T0-eGFP NFATC2IP

Plasmid

#221433

Purpose

Mammalian expression vector of eGFP tagged NFATC2IP

Depositor

Daniel Durocher

Article

Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

Insert

NFATC2IP (NFATC2IP Human)

Tags

eGFP

Expression

Mammalian

Mutation

1st Methionine of NFATC2IP deleted

Promoter

CMV

Available Since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT/T0-3xFLAG-NFATC2IP

Plasmid

#221208

Purpose

Mammalian expression vector of triple Flag tagged NFATC2IP

Depositor

Daniel Durocher

Article

Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

Insert

NFATC2IP (NFATC2IP Human)

Tags

3XFlag

Expression

Mammalian

Mutation

1st Methionine deleted

Promoter

CMV

Available Since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-39S Light chain

Plasmid

#216297

Purpose

Expresses anti-HER2 39S Light chain in mammalian cells. To be paired with 39S heavy chain to form the anti-HER2 39S Fab

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

39S Light chain

Use

Affinity Reagent/ Antibody

Tags

Signal peptide

Expression

Mammalian

Promoter

T7 promoter

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MF3958 Light chain

Plasmid

#216304

Purpose

Expresses anti-HER2 MF3958 Light chain in mammalian cells. To be paired with MF3958 heavy chain to form the anti-HER2 MF3958 Fab

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

MF3958 Light chain

Use

Affinity Reagent/ Antibody

Tags

Signal peptide

Expression

Mammalian

Promoter

T7 promoter

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-3xFLAG-TAF5-∆ex8

Plasmid

#209050

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged TAF5 isoform lacking the alternative exon-8 (∆ex8)

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

TAF5 deltaexon-8

Tags

FLAG

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-miniTurbo-TAF5-∆ex8

Plasmid

#209052

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of miniTurbo fused to TAF5 isoform lacking the alternative exon-8 (∆ex8)

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

TAF5 deltaexon-8

Tags

miniTurbo

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-3xFLAG-TAF5-FL_WobbleMut

Plasmid

#209055

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged full length TAF5 isoform mutated to be resistant to TAF5 siRNA

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

TAF5 full length

Tags

FLAG

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-3xFLAG-TAF5-∆ex8_WobbleMut

Plasmid

#209056

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged TAF5 isoform lacking the alternative exon-8 (∆ex8) mutated to be resistant to TAF5 siRNA

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

TAF5 deltaexon-8

Tags

FLAG

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3-TEX264-SIM1_mut-cFlag

Plasmid

#220205

Purpose

Mammalian expression of TEX264 with SIM1 motif mutated, C-terminal Flag

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Tags

Flag

Expression

Mammalian

Mutation

p.111LIDLY>AAAAA; c.331C>G,332T>C,334A&g…

Promoter

CMV

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3-TEX264-SIM2_mut-cFlag

Plasmid

#220206

Purpose

Mammalian expression of TEX264 with SIM2 motif mutated, C-terminal Flag

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Tags

Flag

Expression

Mammalian

Mutation

p.111ILSIW>ALAIA; c.421A>G,422T>C,427T&g…

Promoter

CMV

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3-TEX264-E194A-cFlag

Plasmid

#220207

Purpose

Mammalian expression of TEX264 with E194A mutation, C-terminal Flag

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Tags

Flag

Expression

Mammalian

Mutation

194E>A

Promoter

CMV

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3-TEX264-SHP1mut-cFlag

Plasmid

#220208

Purpose

Mammalian expression of TEX264 with SHP domain mutated, C-terminal Flag

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Tags

Flag

Expression

Mammalian

Mutation

p.280GEGPL>RERPA; c.838G>C,844G>A,850T&g…

Promoter

CMV

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA5-TEX264-SIM1_mut-cGFP

Plasmid

#220212

Purpose

Mammalian expression of TEX264 with SIM1 motif mutated, fused at C-terminus to GFP

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Tags

GFP

Expression

Mammalian

Mutation

p.111LIDLY>AAAAA; c.331C>G,332T>C,334A&g…

Promoter

CMV/TetO

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA5-TEX264-SIM2_mut-cGFP

Plasmid

#220213

Purpose

Mammalian expression of TEX264 with SIM2 motif mutated, fused at C-terminus to GFP

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Tags

GFP

Expression

Mammalian

Mutation

p.111ILSIW>ALAIA; c.421A>G,422T>C,427T&g…

Promoter

CMV/TetO

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5_NLS-Halo-eGFP-BASU

Plasmid

#218449

Purpose

expressed the NLS-Halo-eGFP-BASU fusion protein via stable Flp-In integration

Depositor

Thorsten Stafforst

Article

Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

Insert

NLS-Halo-eGFP-BASU

Expression

Mammalian

Promoter

CMV

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO mTagBFP2-GABARAP

Plasmid

#212110

Purpose

Expression of N-terminally tagged GABARAP with mTagBFP2

Depositor

Dieter Willbold

Article

Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

Insert

mTagBFP2-GABARAP (GABARAP Human, Entacmaea quadricolor)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO eYFP-GABARAP

Plasmid

#212111

Purpose

Expression of N-terminally tagged GABARAP with eYFP

Depositor

Dieter Willbold

Article

Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

Insert

eYFP-GABARAP (GABARAP Aequorea victoria, Human)

Tags

eYFP

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3-LOVPLD-mCherry-OSBP(PH)

Plasmid

#219561

Purpose

Golgi-targeted LOVPLD

Depositor

Jeremy Baskin

Article

Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

Insert

LOVPLD-mCherry-OSBP(PH)

Tags

mCherry

Expression

Mammalian

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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