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We narrowed to 167,150 results for: htt

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Showing: 8851 - 8900 of 167150 results

  1. pAAV_Ef1a_FLEX_mAPEX2_P2A_mRuby2

    Plasmid
    #171938
    Purpose
    Expresses membrane targeted (palmitoylation) APEX2 and mRuby2 as separate proteins
    Depositor
    David Berson
    Insert
    Apex2, mRuby2
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    Ef1a
    Available Since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    171938-plasmid-map-sequence-id-338493

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  3. AAV-syn-NES-jGCaMP8f-WPRE

    Plasmid
    #186035
    Purpose
    AAV-mediated cytosolic expression of jGCaMP8f under the synapsin promoter
    Depositor
    Loren Looger
    Insert
    NES-jGCaMP8f
    Use
    AAV
    Tags
    NES-6xHis
    Expression
    Mammalian
    Promoter
    synapsin
    Available Since
    July 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    186035-plasmid-map-sequence-id-368187

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  4. R777-E220 Hs.RALBP1-nostop

    Plasmid
    #70504
    Purpose
    Gateway ORF clone of human RALBP1 [NM_006788.3] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RALBP1 (RALBP1 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70504-plasmid-map-sequence-id-125953

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  5. pIVTR(T7A)-Cas9n-RT-T2A-PuroR

    Plasmid
    #172296
    Purpose
    In vitro transcription of Cas9n-RT-T2A-PuroR synthetic construct for PRIME editing
    Depositor
    Patrik Verstreken
    Insert
    Cas9n-RT-T2A-PuroR
    Use
    CRISPR
    Mutation
    H840A (Cas9)
    Promoter
    T7 promoter A-initiating
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172296-plasmid-map-sequence-id-340322

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  6. R777-E356 Hs.TSC2-nostop

    Plasmid
    #70640
    Purpose
    Gateway ORF clone of human TSC2 [NM_001114382.1] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    TSC2 (TSC2 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70640-plasmid-map-sequence-id-126089

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  8. pUltra-Smurf-luc-RTTA3

    Plasmid
    #59438
    Purpose
    silence doxycyclin inducible pUltra-dox (also expresses luciferase)
    Depositor
    Yildirim Dogan, Kitai Kim
    Inserts
    luciferase
    Reverse TET-Transactivator 3
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    loxP5171
    Available Since
    Oct. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    59438-plasmid-map-sequence-id-92494

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  9. Pk-SOD1

    Plasmid
    #25574
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Pk-SOD1
    Tags
    His
    Expression
    Bacterial
    Available Since
    Sept. 10, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. pET-(neg27)GFP-GGS9-Cre-6xHis

    Plasmid
    #89253
    Purpose
    Expresses GFP with -27 theoretical charge fused to Cre in E.Coli
    Depositor
    David Liu
    Insert
    (neg27)GFP-GGS9-Cre-6xHis
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    89253-plasmid-map-sequence-id-176140

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  11. pBabepuro-PDGFB

    Plasmid
    #189611
    Purpose
    Retroviral expression vector for PDGFB. stimulates fibroblastic proliferation (Tchenio T, unpublished, 2008)
    Depositor
    Thierry Tchenio
    Insert
    PDGFB (PDGFB Human)
    Use
    Retroviral
    Promoter
    SV40 early promoter
    Available Since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    189611-plasmid-map-sequence-id-381075

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  12. mEos2-ZO1-C-14

    Plasmid
    #57440
    Purpose
    Localization: Tight Junctions, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    ZO1 (TJP1 Human)
    Tags
    mEos2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. pKK1 -pFlp-18::mito4x-GCaMP6f

    Plasmid
    #194969
    Purpose
    Fluorescent reporter of mitochondrial matrix Ca2+.
    Depositor
    Frederic Hoerndli
    Insert
    4 repeats of Cox8 presequence plus GCaMP6f
    Expression
    Worm
    Promoter
    Flp-18
    Available Since
    June 21, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    194969-plasmid-map-sequence-id-388699

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  14. EF1a CHR2 mCherry WPRE

    Plasmid
    #129432
    Purpose
    expression of CHR2 mCherry from EF1a promoter
    Depositor
    Oskar Laur
    Insert
    CHR2 mCherry
    Use
    Lentiviral
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    129432-plasmid-map-sequence-id-269136

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  15. mTurquoise2-MyosinIIB-C-18

    Plasmid
    #55591
    Purpose
    Localization: Cytoskeleton, Excitation: 434, Emission: 474
    Depositor
    Michael Davidson
    Insert
    MyosinIIB (MYH10 Human)
    Tags
    mTurquoise2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. pDSP23

    Plasmid
    #199372
    Purpose
    Encodes dual cistronic nuclear localized GCaMP6s and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    NLS::GCaMP6s::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Available Since
    April 25, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199372-plasmid-map-sequence-id-390928

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  18. pHAGE2-RSV-jGCaMP7s-W

    Plasmid
    #126690
    Purpose
    Mammalian calcium sensor protein.
    Depositor
    Darrell Kotton
    Insert
    jGCaMP7s
    Use
    Lentiviral
    Tags
    6xHIS, T7, Xpress
    Mutation
    A52V, A317L
    Promoter
    RSV
    Available Since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    126690-plasmid-map-sequence-id-245673

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  19. pDONR221-olig2

    Plasmid
    #192743
    Purpose
    Gateway entry vector encoding zebrafish olig2
    Depositor
    Summer Thyme
    Insert
    olig2 (olig2 Zebrafish)
    Use
    Gateway entry vector
    Mutation
    S61A (rs501471300); G235S (rs505716173)
    Promoter
    None
    Available Since
    Nov. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192743-plasmid-map-sequence-id-377799

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  20. mTFP1-mKO-FRET-8

    Plasmid
    #58152
    Purpose
    Localization: Cytosolic FRET, Excitation: 462 / 548, Emission: 492 / 561
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mKO and mTFP1
    Expression
    Mammalian
    Available Since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    58152-plasmid-map-sequence-id-218288

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  21. sCFP in pBSDONR P4r-P2

    Plasmid
    #121530
    Purpose
    For making C-terminal sCFP fusions using multisite Gateway cloning
    Depositor
    Roger Innes
    Insert
    sCFP
    Expression
    Bacterial
    Available Since
    Aug. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    121530-plasmid-map-sequence-id-234035

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  22. Col2a1:TFPnls-mErt-Cre-Ert

    Plasmid
    #111152
    Purpose
    Chondrocyte specific inducible Cre
    Depositor
    Elly Tanaka
    Insert
    TFPnls-T2a-mErt-Cre-Ert
    Use
    Cre/Lox
    Mutation
    wt
    Available Since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    111152-plasmid-map-sequence-id-211539

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  24. R777-E165 Hs.PIK3CB

    Plasmid
    #70449
    Purpose
    Gateway ORF clone of human PIK3CB [NM_006219.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PIK3CB (PIK3CB Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70449-plasmid-map-sequence-id-125898

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  25. pBluescript mouse ptc

    Plasmid
    #58699
    Purpose
    full length mouse ptc cDNA
    Depositor
    Matthew Scott
    Insert
    ptc (Ptch1 Mouse)
    Use
    Cloning vector
    Promoter
    T7
    Available Since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  26. pLV Tor1a-V5

    Plasmid
    #175135
    Purpose
    Lentiviral expression of Tor1a-Myc
    Depositor
    Larry Gerace
    Insert
    Tor1a (Tor1a Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175135-plasmid-map-sequence-id-352022

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  28. pSMPUW_6.11_M3_Ef1a-gateway-HA-SV40

    Plasmid
    #128447
    Purpose
    lentiviral destination vector with Ef1a promoter, C-terminal HA tag, and SV40 promoter
    Depositor
    Lindy Barrett
    Insert
    Ef1a promoter, C-terminal HA tag, and SV40 promoter
    Use
    Lentiviral
    Available Since
    Sept. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    128447-plasmid-map-sequence-id-256451

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  29. pHAGE2-RSV-jGCaMP7f-W

    Plasmid
    #126691
    Purpose
    Mammalian calcium sensor protein.
    Depositor
    Darrell Kotton
    Insert
    jGCaMP7f
    Use
    Lentiviral
    Tags
    6xHIS, T7, Xpress
    Mutation
    H78K, A317L
    Promoter
    RSV
    Available Since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    126691-plasmid-map-sequence-id-245678

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  31. pMC6-TEFkFLP(LYS2)

    Plasmid
    #49458
    Purpose
    overespression of Flp step-arrest mutant in yeast, LYS2 selection
    Depositor
    Marc Gartenberg
    Insert
    Flp(H305L) (flp Budding Yeast)
    Expression
    Yeast
    Mutation
    H305L, ‘step-arrest’ mutant,
    Promoter
    TEF1
    Available Since
    Dec. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    49458-plasmid-map-sequence-id-73670

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  33. pAAV_hsyn_FLEX_tau-APEX2

    Plasmid
    #171937
    Purpose
    Expresses APEX2 linked to tau for labeling microtubules
    Depositor
    David Berson
    Insert
    tau-APEX2
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    hsyn
    Available Since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    171937-plasmid-map-sequence-id-338490

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  34. NARS_pNIC-Bio3

    Plasmid
    #153054
    Purpose
    Expression construct for producing Avi tagged Asparaginyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    NARS (NARS Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153054-plasmid-map-sequence-id-304473

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  35. pIC81

    Plasmid
    #63065
    Purpose
    Mammalian expression of EGFP-TEV-S-tag PMFI for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    PMFI, IMAGE clone 6148250 (PMF1 Human)
    Tags
    EYFP and TEV-S
    Expression
    Mammalian
    Available Since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    63065-plasmid-map-sequence-id-104380

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  36. pJK266

    Plasmid
    #73326
    Purpose
    Produces Janthinobacterium lividum violacein biosynthesis protein VioD with a N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    violacein biosynthesis protein VioD
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    73326-plasmid-map-sequence-id-135034

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  37. mApple-Moesin-N-14

    Plasmid
    #54928
    Purpose
    Localization: Cytoskeleton/Membrane, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    Moesin (MSN Human)
    Tags
    mApple
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  38. LHP336 - HA-Ub-K48R

    Plasmid
    #32164
    Depositor
    Linda Hicke
    Insert
    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    Tags
    HA
    Expression
    Yeast
    Mutation
    K48R
    Promoter
    CUP1
    Available Since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  42. Dronpa-Peroxisomes-2

    Plasmid
    #54690
    Purpose
    Localization: Peroxisomes, Excitation: 503, Emission: 518
    Depositor
    Michael Davidson
    Insert
    Dronpa-Peroxisomes
    Tags
    Peroxisomal Targeting Signal 1 (PTS1; Ser-Lys-Leu…
    Expression
    Mammalian
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  43. pIVTR(T7A)-CRE-T2A-PuroR

    Plasmid
    #172298
    Purpose
    In vitro transcription of CRE-T2A-PuroR synthetic construct for the excision of floxed cassettes
    Depositor
    Patrik Verstreken
    Insert
    CRE-T2A-PuroR
    Use
    Cre/Lox
    Promoter
    T7 promoter A-initiating
    Available Since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    172298-plasmid-map-sequence-id-340323

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  44. R777-E246 Hs.RASGRP2-nostop

    Plasmid
    #70530
    Purpose
    Gateway ORF clone of human RASGRP2 [NM_153819.1] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RASGRP2 (RASGRP2 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70530-plasmid-map-sequence-id-125979

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  45. PB CAG-L-bpA-SV40pA-L-td-sfGFP

    Plasmid
    #133575
    Purpose
    CAG tdGFP cre/lox reporter piggyBac transposon (bpA 1x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Cre/Lox
    Expression
    Mammalian
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133575-plasmid-map-sequence-id-274068

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  46. pICSL4723_ERF922

    Plasmid
    #126883
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    126883-plasmid-map-sequence-id-250052

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  47. L4665

    Plasmid
    #1667
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    myo3-2341, myo3Tsa, IVSA, rf2128, ATG-MitLS, gfpN…
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1667-plasmid-map-sequence-id-277

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  48. PP-HSP90 (3Q5J)

    Plasmid
    #28281
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-HSP90
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    N-terminal domain
    Available Since
    Aug. 8, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  49. pDEST26-hTLR3-Nluc

    Plasmid
    #197638
    Purpose
    Human CD283 protein expression in mammalian cells
    Depositor
    Haian Fu
    Insert
    TLR3 (TLR3 Human)
    Tags
    NanoLuciferase
    Expression
    Mammalian
    Available Since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    197638-plasmid-map-sequence-id-392617

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  50. mRuby-Peroxisomes-2

    Plasmid
    #54840
    Purpose
    Localization: Peroxisomes, Excitation: 558, Emission: 605
    Depositor
    Michael Davidson
    Insert
    mRuby-Peroxisomes
    Tags
    Peroxisomal Targeting Signal 1 (PTS1; Ser-Lys-Leu…
    Expression
    Mammalian
    Available Since
    July 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 8851 - 8900 of 167150 results