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pNeoXTR f2

Plasmid

#69159

Purpose

mouse targeting vector

Depositor

David Feldser

Article

Robles-Oteiza et al Nat Commun. 2015 Nov 5;6:8783. doi: 10.1

Inserts

DTA
NeoR
GFP
AmpR

Use

Mouse Targeting

Available Since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pRMCE{G418R-5FCS-GMRWhite}-Vasa-FC31

Plasmid

#165887

Purpose

G418 resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertions

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

All-in-one FC31 RMCE Cassette

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1α-Cre-2A-miRFP670nano3

Plasmid

#207747

Purpose

Expression of the Cre recombinase linked to a miRFP670nano3 fluorescent marker

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

Cre-PGK-miRFP670nano3

Use

Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG-GFPmG1

Plasmid

#50444

Purpose

Expression of a EGFP variant in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

GFPmG1

Expression

Mammalian

Mutation

E143K, N147Q

Promoter

CAG

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-RAMP4-IRES-Puromycin

Plasmid

#134863

Purpose

Expresses EGFP-tagged endoplasmic reticulum marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

RAMP4 (SERP1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCO603

Plasmid

#139736

Purpose

individual component of Fungal Bioluminescent Pathway, MoClo level 0 plasmid, NnLuz

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

Luz

Use

Luciferase; Moclo level 0 component

Promoter

none

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pM198

Plasmid

#173218

Purpose

Expresses OptGFP(1-9)-GB1

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Insert

OptGFP(1-9)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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LacI INCENP

Plasmid

#45236

Purpose

Target INCENP to lac operator sequence

Depositor

Michael Lampson

Article

Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

Insert

LacI, INCENP-Δcen, mCherry (lacI )

Tags

LacI (lac repressor protein) and mCherry

Expression

Mammalian

Mutation

CEN box deleted from INCENP (aa1-46)

Promoter

t7

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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p3A9cbetaTCR

Plasmid

#69600

Purpose

TCR beta chain transgene expression plasmid which has a unique ClaI site for the insertion of a TCR beta chain VDJ exon

Depositor

Francis R. Carbone

Article

Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only
pCO600

Plasmid

#139734

Purpose

individual component of Fungal Bioluminescent Pathway, MoClo level 0 plasmid, NnHisps

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

Hisps

Use

Luciferase; Moclo level 0 component

Promoter

none

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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P212

Plasmid

#139718

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, Luz

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_D_p35S:NnLuz:tAtuOcs

Use

Luciferase

Expression

Plant

Promoter

35S

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pPpT4- PGK1prom-TIR1-FLAG

Plasmid

#189724

Purpose

Enables expression of OsTIR1 from the PGK1 promoter, used to implement the AID system in Komagataella phaffii (original name pAEA454)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Expression

Yeast

Promoter

PGK1 promoter

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLJM1-Clover-Prx2-mRuby2

Plasmid

#117907

Purpose

fluorescent reporter of cytosolic hydrogen peroxide

Depositor

Hadley Sikes

Article

Langford et al Nat Commun. 2018 Aug 7;9(1):3145. doi: 1

Insert

Clover-Prx2-mRuby2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

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pGolden-Hyg

Plasmid

#51423

Purpose

Contain one loxP-PGK-Hyg-pA-loxP cassette, used for golden-gate reaction

Depositor

Yonglun Luo

Article

Luo et al Biotechniques. 2014 May 1;56(5):263-8. d

Insert

PGK-Hyg-pA (PGK1 Human)

Expression

Mammalian

Promoter

PGK

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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P368

Plasmid

#139723

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, H3H

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_B_pAtuMas:NnH3H:tAtuMas

Use

Luciferase

Expression

Plant

Promoter

AtuMas

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pICH-roGFP2-GPXC71S

Plasmid

#191698

Purpose

Ti plasmid pICH86988 harbouring roGFP2-GPXC71S -a control roGFP2 fusion not responsive to peroxides used alongside plasmids 191680 and 191677

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

Insert

redox(ro)GFP2-GPXC71S

Expression

Plant

Mutation

Peroxidatic Cysteine 71 replaced with Serine

Promoter

CaMV 35S

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pMK455 (dTAG-mClover-BSD)

Plasmid

#214385

Purpose

dTAG-mClover for C-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

dTAG-mClover-BSD

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMK456 (dTAG-mCherry-BSD)

Plasmid

#214386

Purpose

dTAG-mCherry for C-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

dTAG-mCherry-BSD

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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LLhG-pHG165a E62K

Plasmid

#90051

Purpose

Constitutive expression of a chimeric LacI:GalR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Proteins. 2008 Dec;73(4):941-57. doi: 10

Insert

LLhG/E62K

Expression

Bacterial

Mutation

E62K

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pFCK(1.3)GFP-SynaptophysW

Plasmid

#27235

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

Synaptophys-GFP

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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