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We narrowed to 9,857 results for: EPO

Showing: 3541 - 3560 of 9857 results

  3. pCJ189

    Plasmid
    #162666
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162666-plasmid-map-sequence-id-321465

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  7. AICSDP-49: MYL2-mEGFP

    Plasmid
    #114414
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human MYL2, via Cas9-excisable CAGGS-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114
    Insert
    MYL2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (MYL2 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available Since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    114414-plasmid-map-sequence-id-220893

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  9. pZ P (cHS4)X4 OCT4p eGFP SV40pA v2

    Plasmid
    #115518
    Purpose
    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows OCT4 dependent expression of GFP, and contains cHS4 insulators.
    Depositor
    Catherine Verfaillie
    Article
    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-
    Insert
    eGFP
    Use
    AAV; Donor plasmid for recombinase-mediated casse…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    115518-plasmid-map-sequence-id-229325

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  13. A3Bi-ctd-Cas9n-UGI-NLS

    Plasmid
    #109426
    Purpose
    Expresses the C-terminal catalytic domain of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
    Depositor
    Reuben Harris
    Article
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Insert
    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Insertion of an L1 intron into the coding sequenc…
    Promoter
    CMV
    Available Since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    109426-plasmid-map-sequence-id-212924

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  14. AICSDP-46: MYL7-mEGFP

    Plasmid
    #114413
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human MYL7, via Cas9-excisable CAGGS-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114
    Insert
    MYL7 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (MYL7 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available Since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    114413-plasmid-map-sequence-id-250980

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  15. pCJ208

    Plasmid
    #162681
    Purpose
    Lidded variant of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) removing a seven-residue loop of PETase (Trp185:Phe191, PETase numbering) and replacing it with Gly251:Thr472 from MHETase; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) incorporating the MHETase lid
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Replacement of W185-F191 with G251-T472 from MHET…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162681-plasmid-map-sequence-id-321444

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  16. pCJ191

    Plasmid
    #162668
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162668-plasmid-map-sequence-id-321523

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  19. pZ P TetON-3XFLAG-tdT CAGG-m2rtTA v2

    Plasmid
    #112668
    Purpose
    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression of your protein of interest.
    Depositor
    Catherine Verfaillie
    Article
    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-
    Insert
    tdT
    Use
    AAV; Donor plasmid for recombinase-mediated casse…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    112668-plasmid-map-sequence-id-229324

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  20. ptdTHC_PGKneoLox2DTA.2

    Plasmid
    #112625
    Purpose
    H2BtdTomato_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-tdTomato (H2BC3 Anthozoa, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Cre/Lox and Mouse Targeting
    Tags
    tdTomato
    Expression
    Mutation
    Promoter
    No Promoter
    Available Since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112625-plasmid-map-sequence-id-222029

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Showing: 3541 - 3560 of 9857 results