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Sema3c(L)-AP-His

Plasmid

#72015

Purpose

Expresses the Sema3C protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3c (Sema3c Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pFNC-3

Plasmid

#233296

Purpose

Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. CmR, easily curable via sucrose counterselection.

Depositor

Pablo Ivan Nikel

Article

Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

Inserts

BxbI phage integrase
sacB

Expression

Bacterial

Promoter

PEM7 and unknown

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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Plxnb1(L)-Fc-His

Plasmid

#72126

Purpose

Expresses the entire extracellular region of the PlexinB1 protein (ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxnb1 (Plxnb1 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS2

Plasmid

#127127

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS2 (RPS2 Human)

Use

Lentiviral

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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Sema6c-AP-His

Plasmid

#72041

Purpose

Expresses the extracellular region of the Sema6C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6c (Sema6c Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMIR-Dsred-IRES-His-Halo-Tev-Keap C288S

Plasmid

#62459

Purpose

expresses his-tagged C288S human Keap1 protein

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Tags

His-Halo-Tev

Expression

Mammalian

Mutation

Changed Cysteine 288 of Keap1 to serine

Promoter

CMV

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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p21-pRc/CMV

Plasmid

#20814

Depositor

William Kaelin

Article

Adams et al Mol Cell Biol. 1996 Dec . 16(12):6623-33

Insert

p21 (CDKN1A Human)

Expression

Mammalian

Mutation

wt

Available Since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

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MAC_C_LAMP1

Plasmid

#111669

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

LAMP1 (LAMP1 Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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YFP-Rpp29-C1

Plasmid

#134545

Purpose

Expresses YFP-Rpp29 fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

Rpp29 (POP4 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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Sema6d.4-AP-His

Plasmid

#72044

Purpose

Expresses the extracellular region of the Sema6D, isoform 4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6d.4 (Sema6d Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pHS1861

Plasmid

#205974

Purpose

FLAG-SV40 NLS-Cas8-HNH from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas8-HNH

Tags

FLAG-SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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PZac2.1 hSyn1-HA-BioID2-SAPAP3

Plasmid

#176857

Purpose

Expresses SAPAP3 protein fused with BioID2 at the N-terminus in neurons

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

HA-BioID2-SAPAP3

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

hSyn1

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLX302-JSP1-V5 puro

Plasmid

#87773

Purpose

Expresses V5 tagged human JSP1 (DUSP22)

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

JSP1 (DUSP22 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

March 23, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-T7-hOsa1

Plasmid

#17986

Depositor

Naoko Tanese

Article

Inoue et al J Biol Chem. 2002 Nov 1. 277(44):41674-8

Insert

Osa1 (ARID1A Human)

Tags

T7 epitope

Expression

Mammalian

Mutation

Missing 5' end

Available Since

Aug. 19, 2008

Availability

Academic Institutions and Nonprofits only
pCI-neo VPS50-13Myc

Plasmid

#175093

Purpose

13Myc tagged VPS50 is expressed in mammalian cells

Depositor

Juan Bonifacino

Article

Schindler et al Nat Cell Biol. 2015 May;17(5):639-50. do

Insert

VPS50 (CCDC132 Human)

Tags

13Myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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moPrP(3F4) D177N

Plasmid

#1319

Depositor

Susan Lindquist

Article

Ma et al Proc Natl Acad Sci U S A 2001 Dec 18;98(

Insert

PrP D177N (Prnp Mouse)

Expression

Mammalian

Mutation

D177N point mutation

Available Since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only
mSecPrP-pCB6+

Plasmid

#13917

Depositor

Susan Lindquist

Article

Ma et al Proc Natl Acad Sci U S A 2001 Dec 18;98(

Insert

PrP (Prnp Mouse)

Expression

Mammalian

Mutation

3F4 epitag

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
Cav2.2 HA pcDNA3

Plasmid

#206051

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCEP4-HA-Flag-natT1R2

Plasmid

#113945

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

residues 22-839

Promoter

CMV

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pKG-GW1

Plasmid

#203163

Purpose

Yeast expression vector; To express proteins under control of TDH3 promoter and terminator using leucine selection

Depositor

William Sessa

Article

Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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