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pJJBAD78

Plasmid

#15344

Depositor

Hideo Iwai

Article

Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

Insert

DnaE C-intein(CMN)

Tags

GB1

Expression

Bacterial

Mutation

F to M mutation

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

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pJJBAD79

Plasmid

#15345

Depositor

Hideo Iwai

Article

Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

Insert

DnaE C-intein(CKN)

Tags

GB1

Expression

Bacterial

Mutation

F to K mutation

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only
pJJBAD81

Plasmid

#15347

Depositor

Hideo Iwai

Article

Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

Insert

DnaE C-intein(CQN)

Tags

GB1

Expression

Bacterial

Mutation

Y to Q mutation

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only
pJJBAD82

Plasmid

#15348

Depositor

Hideo Iwai

Article

Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

Insert

DnaE C-intein(CEN)

Tags

GB1

Expression

Bacterial

Mutation

F to E mutation

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only
pJJBAD83

Plasmid

#15349

Depositor

Hideo Iwai

Article

Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

Insert

DnaE C-intein(CHN)

Tags

GB1

Expression

Bacterial

Mutation

F to H mutation

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only
pJJBAD84

Plasmid

#15350

Depositor

Hideo Iwai

Article

Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

Insert

DnaE C-intein(CIN)

Tags

GB1

Expression

Bacterial

Mutation

F to I mutation

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only
pJJBAD85

Plasmid

#15351

Depositor

Hideo Iwai

Article

Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

Insert

DnaE C-intein(CLN)

Tags

GB1

Expression

Bacterial

Mutation

F to L mutation

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only
pJJBAD86

Plasmid

#15352

Depositor

Hideo Iwai

Article

Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

Insert

DnaE C-intein(CCN)

Tags

GB1

Expression

Bacterial

Mutation

F to C mutation

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only
pJJBAD87

Plasmid

#15353

Depositor

Hideo Iwai

Article

Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

Insert

DnaE C-intein(CSN)

Tags

GB1

Expression

Bacterial

Mutation

F to S mutation

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only
pJJBAD88

Plasmid

#15354

Depositor

Hideo Iwai

Article

Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

Insert

DnaE C-intein(CGN)

Tags

GB1

Expression

Bacterial

Mutation

F to G mutation

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only
pKB254

Plasmid

#170037

Purpose

Encodes LacI-controlled expression of surface-displayed H6L9 and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
H6L9

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Promoter

Ptac and PvirK

Availability

Academic Institutions and Nonprofits only

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pPSU20-40-60-80

Plasmid

#173839

Purpose

Coexpresses 20, 40, 60 and 80 kD Penn State ladder proteins

Depositor

Song Tan

Article

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPABPACCBPv3

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pPSU15

Plasmid

#173828

Purpose

Expresses 15 kD Penn State ladder protein

Depositor

Song Tan

Article

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

HSTPABPAC

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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FUdeltaGW-rtTA

Plasmid

#19780

Purpose

3rd generation lentiviral vector

Depositor

Konrad Hochedlinger

Article

Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

Insert

reverse tetracycline transactivator

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 24, 2009

Availability

Academic Institutions and Nonprofits only

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pHCMV-EcoEnv

Plasmid

#15802

Depositor

Miguel Sena-Esteves

Article

Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

Insert

MLV env (ecotropic)

Expression

Mammalian

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Lysosomes-20

Plasmid

#55308

Purpose

Localization: Lysosome Membrane, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

LAMP1

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pBAD-I-SceI

Plasmid

#60960

Purpose

Plasmid that allows an arabinose-inducible expression of the I-SceI homing endonuclease - for the en passant (two-step Red-mediated) mutagenesis

Depositor

Nikolaus Osterrieder

Article

Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

Insert

I-SceI restriction enzyme

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pNF02-mLychee

Plasmid

#219400

Purpose

Constitutive expression of mLychee in E. coli

Depositor

Christian Lesterlin

Article

Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

Insert

mLychee

Expression

Bacterial

Promoter

proDp

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pEA186

Plasmid

#187874

Purpose

Empty pVS1-based INTEGRATE vector with mCherry cargo

Depositor

Kan Wang

Article

Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pPGKPhiC31obpA

Plasmid

#13795

Purpose

Expression of mouse codon-optimized PhiC31 recombinase with bovine growth hormone polyadenylation (bpA) sequence.

Depositor

Philippe Soriano

Article

Raymond et al PLoS One. 2007 Jan 17;2(1):e162. doi: 10

Insert

PhiC31o

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 16, 2007

Availability

Academic Institutions and Nonprofits only

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mApple-N1

Plasmid

#54567

Purpose

Localization: N1 Cloning Vector, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Type

Empty backbone

Tags

mApple

Expression

Mammalian

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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pNF02-mJuniper

Plasmid

#219398

Purpose

Constitutive expression of mJuniper in E. coli

Depositor

Christian Lesterlin

Article

Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

Insert

mJuniper

Expression

Bacterial

Promoter

proDp

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNF02-mChartreuse

Plasmid

#219397

Purpose

Constitutive expression of mChartreuse in E. coli

Depositor

Christian Lesterlin

Article

Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

Insert

mChartreuse

Expression

Bacterial

Promoter

proDp

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBbA5k-acrAB-sfGFP

Plasmid

#195516

Purpose

Inducible expression vector for transcriptionally fused acrAB and sfGFP.

Depositor

Mary Dunlop

Article

Wen et al Sci Rep. 2018 Oct 11;8(1):15120. doi: 10

Insert

acrAB

Use

Synthetic Biology

Tags

sfgfp

Expression

Bacterial

Promoter

lacUV5

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pNF02-mLemon

Plasmid

#219399

Purpose

Constitutive expression of mLemon in E. coli

Depositor

Christian Lesterlin

Article

Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

Insert

mLemon

Expression

Bacterial

Promoter

proDp

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pKOS6b-KOpstS

Plasmid

#237503

Purpose

Knockout of pstS

Depositor

Buz Barstow

Insert

phosphate ABC transporter substrate-binding protein PstS

Expression

Bacterial

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTKEI-Tre-C04

Plasmid

#79754

Purpose

Single fluorescent protein biosensor for trehalose

Depositor

David Savage

Article

Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

Insert

Tre-C04

Tags

6xHis

Expression

Bacterial

Promoter

pTrc

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pFlare9A-Dup34

Plasmid

#90267

Purpose

Minigene reporter for alternative splicing analysis

Depositor

Douglas Black

Article

Stoilov et al Proc Natl Acad Sci U S A. 2008 Aug 12;10

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pFlare5GdP

Plasmid

#90250

Purpose

Minigene reporter for alternative splicing analysis

Depositor

Douglas Black

Article

Zheng et al Genome Res. 2013 Jun;23(6):998-1007. doi

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pSUPERretro-Sirt6 shRNA2

Plasmid

#53148

Purpose

shRNAi vector for Sirt6

Depositor

Katrin Chua

Article

Tennen et al Nat Commun. 2011 Aug 16;2:433. doi: 10.1

Insert

Sirt6

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

H1 RNA polymerase III

Available Since

May 9, 2014

Availability

Academic Institutions and Nonprofits only

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