We narrowed to 15,089 results for: pET
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Plasmid#133230PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter Rlv3841pnifH (pOGG082), mCherry (EC15071) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5-MYC-KPTN
Plasmid#87043Purposemammalian expression of KPTNDepositorAvailable SinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVRa12_807
Plasmid#49663DepositorInsertECF12_807
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-Hs4EBP3_Z
Plasmid#148151PurposeMammalian Expression of Hs4EBP3DepositorInsertHs4EBP3 (EIF4EBP3 Human)
ExpressionMammalianAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJT112_GalL_nCDA1-xBE3
Plasmid#141318PurposeExpressing base editor nCDA1-xBE3 in yeast cellsDepositorInsertnCDA1-xBE3
UseCRISPRExpressionYeastMutationspCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…PromoterGalLAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
PAX6 sgRNA2
Plasmid#68465Purposetargeting PAX6 geneDepositorAvailable SinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAM4896 (pCV0008)
Plasmid#120081PurposeBroad host range plasmid. Bacteria plasmid vector to test various parts and devices. Expression of GFP and a nourseothricin resistance gene in various cyanobacteria.DepositorInsertPlasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-nat1_A7120, CV-PconII-LTRBS-GFPmut2
UseOtherMutationmobA Y25FAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV.U6.gLacZ.Cas9-T2A-GFP
Plasmid#170361PurposeNegative control plasmid used for Crispr/cas9 based disruption.DepositorInsertCas9-T2A-GFP
UseLentiviralPromoterU6Available SinceJune 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAM4889 (pCV0001)
Plasmid#120079PurposeBroad host range plasmid. Bacteria plasmid vector to test various parts and devices. Expression of GFP and a spectinomycin/streptomycin resistance gene in various cyanobacteria.DepositorInsertPlasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-aadA, CV-PconII-LTRBS-GFPmut2
UseOtherMutationmobA Y25FAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmcsg7-mCRT(deltaP)
Plasmid#83507PurposeBacterial expression of Calreticulin mutantDepositorInsertCALR (Calr Mouse)
TagsHisExpressionBacterialMutationdelta . Deletion of residues 187-283PromoterT7Available SinceOct. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmcsg7-mCRT(deltaC)
Plasmid#83506PurposeBacterial expression of Calreticulin mutantDepositorInsertCALR (Calr Mouse)
TagsHisExpressionBacterialMutationdelta C. Contains residues 1-339PromoterT7Available SinceOct. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXJ40 GADD34 (214-513aa)
Plasmid#78095Purposemammalian expression of GADD34 214-513 aaDepositorInsertGADD34 (PPP1R15A Human)
TagsFLAGExpressionMammalianMutationexpresses aa 214-513PromoterCMVAvailable SinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVRa15_436
Plasmid#49667DepositorInsertECF15_436
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX330_rActb sgRNA / hSpCas9
Plasmid#172833PurposeMammalian expression of a sgRNA targeting the intron 1of rat Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of rActb under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVRa28_1088
Plasmid#49683DepositorInsertECF28_1088
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV.U6gT28.13.EFS-NS.H2B-RFP
Plasmid#170388PurposePlasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Expressing nuclear RFP.DepositorInsertH2B-RFP
UseLentiviralPromoterEFS-NSAvailable SinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_Tuba1b sgRNA / hSpCas9
Plasmid#172835PurposeMammalian expression of a sgRNA targeting the intron 1 of Tuba1b (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of Tuba1b under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_rSyn1 sgRNA / hSpCas9
Plasmid#172840PurposeMammalian expression of a sgRNA targeting the intron 21 (last intron) of rSyn1 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 21 of rSyn1 under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVRa11_987
Plasmid#49661DepositorInsertECF11_987
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
Zebrafish-gRNA-0001
Plasmid#42241PurposegRNA targeted to zebrafish gene apoeaDepositorAvailable SinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/SH/GW-Mst1
Plasmid#84299PurposeExpresses a tetracycline-inducible SH-tagged (streptavidin-binding peptide and HA tag) version of Mst1. Used in conjunction with Flp-In cells to generate single copy transgene.DepositorAvailable SinceOct. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJT45_GalL_nCDA1-BE3
Plasmid#145038PurposeExpresses nCDA1-BE3 in yeast cellsDepositorInsertnCDA1-BE3
UseCRISPRExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
WPXL LEFTY2 gRNA
Plasmid#101924PurposeLEFTY2 gRNA used for targeted demethylation is inserted into the WPXL lentiviral backbone.DepositorInsertLEFTY2 enhancer targeting gRNA
UseLentiviralAvailable SinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Ywhag-3'UTR-MRE-3 MUT
Plasmid#61792PurposeLuciferase reporter containing the 3'UTR of mouse Ywhag with MRE-3 mutatedDepositorInsertmouse Ywhag 3'UTR with miR-200c MRE-3 mutated
UseLuciferaseMutationmiR-200c MRE-3 mutatedAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
mADAM8 deltaMP in pQCXIP cFLAG
Plasmid#19149DepositorAvailable SinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pVRa15_524
Plasmid#49668DepositorInsertECF15_524
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-Puro-CTRLg2 (CG510)
Plasmid#139456PurposeLentiviral vector with non-targeting gRNA and puromycin selectable markerDepositorInsertNon-targeting sgRNA inserted; resistance gene: puroR
UseLentiviralExpressionMammalianPromoterEF-1a / U6Available SinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT79_GalL_nCDA1Δ192-BE3
Plasmid#145045PurposeExpresses nCDA1Δ192-BE3 in yeast cellsDepositorInsertnCDA1Δ192-BE3
UseCRISPRExpressionYeastMutationpmCDA1(1-192AA); spCas9(D10A)PromoterGalLAvailable SinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
Zebrafish-gRNA-0002
Plasmid#42242PurposegRNA targeted to zebrafish gene drd3DepositorAvailable SinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pVRa17_1691
Plasmid#49672DepositorInsertECF17_1691
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
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WPXL SOX10 gRNA
Plasmid#101923PurposeSOX10 gRNA used for targeted demethylation is inserted into the WPXL lentiviral backbone.DepositorInsertSOX10 promoter targeting gRNA
UseLentiviralAvailable SinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVRa37_2513
Plasmid#49696DepositorInsertECF37_2513
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pfast bac-flag Fbxl3
Plasmid#51894PurposeBackbone plasmid is pfastBacHTa from invitrogenDepositorAvailable SinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUASp-Flag-Myc-Qin-E3-attB
Plasmid#44209DepositorInsertQin (qin Fly)
UseFly transformationTags3xFlag and 6xMycMutationlacking 5xTudor domains on C-term; aa1-274 onlyAvailable SinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pVRa07_980
Plasmid#49659DepositorInsertECF07_980
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRa30_83
Plasmid#49686DepositorInsertECF30_83
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N1-hRIP3-FL VV458/460PP
Plasmid#61368PurposeExpresses Human full length RIPK3 with A458P/V460P mutation in the mammalian cellsDepositorInsertFull length human RIP3 with VV458/460P mutation (RIPK3 Human)
TagsEYFPExpressionMammalianMutationchanged V458 to P and V460to PAvailable SinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-hRIP1-FL I539P
Plasmid#61380PurposeExpresses Human full length RIPK1with I539P mutation in the mammalian cellsDepositorInsertFull length human RIP1with I539P mutation (RIPK1 Human)
TagsEGFP and HA tagExpressionMammalianMutationchanged I539 to PAvailable SinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVRa16_973
Plasmid#49669DepositorInsertECF16_973
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRa11_3726
Plasmid#49662DepositorInsertECF11_3726
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
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