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pHS1528

Plasmid

#205982

Purpose

U6-MWEE01000013 Cas5-HNH DR-BpiI-DR

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas5-HNH DR-BpiI-DR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE N-mRuby3 (from SARS-CoV-2) IRES puro

Plasmid

#170466

Purpose

The plasmid codes for the Nucleoprotein (N) of SARS-CoV-2 fused to a mRuby3 fluorescent protein in its C-terminus. Inserted in a lentiviral backbone. Puromycin resistance

Depositor

Raphael Gaudin

Article

Bakhache et al bioRxiv 2021

Insert

Nucleocapsid

Use

Lentiviral

Tags

mRuby3

Expression

Mammalian

Promoter

EF1

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX2T VPS4B, residues 1-86

Plasmid

#180639

Purpose

Bacterial expression for VPS4B MIT domain, residues 1-86. Has N-terminal GST tag. Internal ID: WISP04-155.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

VPS4B (VPS4B Human)

Tags

GST

Expression

Bacterial

Mutation

Residues 1-86

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCB128

Plasmid

#205137

Purpose

Expression of mCherry-2xFYVE in Dictyostelium

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

2xFYVE (from human Hrs) (HGS Synthetic)

Use

Dictyostelium expression

Tags

mCherry

Available Since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pFETCh_CIC_iso1

Plasmid

#135728

Purpose

Donor vector for 3' FLAG tag of human CIC_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

CIC_iso1 Homology arms (CIC Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pTYF-sGFAP-LOx-IRES-tdTomato

Plasmid

#166919

Purpose

Express the bacterial lactate oxidase (LOx) enzyme in astrocytes

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

Insert

Lactate oxidase

Use

Lentiviral

Tags

tdTomato

Expression

Mammalian

Promoter

sGFAP

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_NTD-ZFN_L-HiBiT

Plasmid

#198319

Purpose

Loads HiBiT-tagged L fragment of a zinc finger nuclease specific for human AAVS1 locus using Pdp1 packaging domain

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-ZFN_L-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCI Flag Smad3 LC EPSM

Plasmid

#27038

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 133-425; T179V S204A S208A S213A

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
MDO2-c003

Plasmid

#173105

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

MACROD2 (MACROD2 Human)

Expression

Bacterial

Mutation

K7-D243

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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MDO2-c005

Plasmid

#173106

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

MACROD2 (MACROD2 Human)

Tags

Nanoluc

Expression

Bacterial

Mutation

K7-D243

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pY019 (pcDNA3.1-hPmCpf1)

Plasmid

#69991

Purpose

Expresses humanized PmCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hPmCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only
pBabe_puro_DEST_Flag_SLC2A2

Plasmid

#45267

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

SLC2A2 (SLC2A2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pCI Flag Smad3 NL EPSM

Plasmid

#27006

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 1-226; T179V S204A S208A S213A

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
JAB2603

Plasmid

#190390

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_5XAmtRO::GFPint-NLS-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_5XAmtRO::GFPint-NLS-ADHt

Expression

Plant

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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pHES873

Plasmid

#87945

Purpose

Expresses estradiol-inducible synthetic transcription factor in yeast under control of GAL1 promoter. It activates transcription of promoters containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pGAL1- Zif268 DBD - hPR LBD - MSN2 AD (PGR Budding Yeast, Human)

Expression

Yeast

Promoter

GAL1

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP120-TD-HilA

Plasmid

#153330

Purpose

Triggers secretion of SptP120 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP120-HA4-7c12 Tandem Monobody

Use

Salmonella expression

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pJ414-PCY1

Plasmid

#98150

Purpose

Expresses PCY1 from Saponaria vaccaria possessing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1

Tags

His6-TEV

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP167-3G124-HilA

Plasmid

#153322

Purpose

Triggers secretion of SptP167 fused to the 3G124nc anti-GFP DARPin from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP167-3G124 DARPin

Use

Salmonella expression

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pBstSK+IRES-V5-IPpoI

Plasmid

#206321

Purpose

A plasmid that facilitates production of mRNA encoding V5 epitope tagged I-PpoI

Depositor

Brian McStay

Article

van Sluis et al Genes Dev. 2015 Jun 1;29(11):1151-63. do

Insert

IRES element-V5 epitope tag- IPpoI open reading frame

Use

Mrna production

Tags

V5-IPpoI fusion protein

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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PX458_CIC_iso1_2

Plasmid

#135744

Purpose

Encodes gRNA for 3' target of human CIC_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

CIC_iso1 gRNA (CIC Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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