We narrowed to 18,431 results for: Met

1. Gi-c009

   Plasmid

   #173079

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   GNAI1 (GNAI1 Human)
   

   Tags

   mCitrine

   Expression

   Bacterial

   Mutation

   full length, codon optimized

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-SMVP-Cas9N-U6-gRNA TdL

   Plasmid

   #80938

   Purpose

   Expresses Cas9N in mammalian cells; expresses gRNA TdL for Ai9 cleavage.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9N
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEB2-mNeptune2

   Plasmid

   #104012

   Purpose

   Plasmid encoding mNeptune2

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mNeptune2
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   Mutations relative to eqFP578 (MSKGEE LIKENM… R32…

   Promoter

   proC

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. POT4-RFP

   Plasmid

   #65320

   Purpose

   receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   RFP
   

   Use

   Synthetic Biology

   Available Since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-bArrestin2-TevC-P2A-TdTomato

   Plasmid

   #89869

   Purpose

   expresses iTango bArrestin2-TevC component and TdTomato bicistronically

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   bArrestin2-TevC-P2A-TdTomato
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Mutation

   R173H in TdTomato (please see depositor comments …

   Promoter

   CMV

   Available Since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSFDuet-1_StefinA

   Plasmid

   #182402

   Purpose

   Bacterial expression of stefinA

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   stefin (CSTA Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. cmv-sfGFP-GB1-scAB-ubc-nls-pcp-5xSunTag(SRv1-2P)

   Plasmid

   #185800

   Purpose

   one plasmid encoding scFv GFP and pcp-5xSunTag components for SunRISER SRv1

   Depositor

   Robin Lee

   Article

   Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

   Insert

   cmv-sfGFP-GB1-scAB--ubc-nls-pcp-5xSunTag
   

   Expression

   Mammalian

   Available Since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. NELFE-mOrange pHis-//

   Plasmid

   #53303

   Purpose

   Expresses His-tagged NELF-E-mOrange fusion protein.

   Depositor

   John Lis

   Article

   Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

   Insert

   NELF-E protein (Nelf-E Fly)
   

   Tags

   His and mOrange

   Expression

   Bacterial

   Available Since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. Dronpa-Mito-7

   Plasmid

   #57288

   Purpose

   Localization: Mitochondria, Excitation: 503, Emission: 518

   Depositor

   Michael Davidson

   Article

   Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

   Insert

   Mito (COX8A Human)
   

   Tags

   Dronpa

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 13, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pCaSpeR4 mito roGFP2-Orp1

    Plasmid

    #65001

    Purpose

    Expression of mitochondrial roGFP2-Orp1 under the alpha Tub84B promoter

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Tags

    roGFP2

    Expression

    Insect

    Mutation

    mitochondrial targeting sequence

    Available Since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. mIFP12-sEspin-C-18

    Plasmid

    #56262

    Purpose

    Localization: Actin Bundling, Excitation: 683, Emission: 704

    Depositor

    Michael Davidson

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Insert

    sEspin
    

    Tags

    mIFP12

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. CoV-NSP3-c001

    Plasmid

    #173082

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    rep
    

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    E1000-K1173, codon optimized

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. MDO2-c003

    Plasmid

    #173105

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    MACROD2 (MACROD2 Human)
    

    Expression

    Bacterial

    Mutation

    K7-D243

    Available Since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. MDO2-c005

    Plasmid

    #173106

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    MACROD2 (MACROD2 Human)
    

    Tags

    Nanoluc

    Expression

    Bacterial

    Mutation

    K7-D243

    Available Since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. mOrange2-Tubulin-6

    Plasmid

    #57974

    Purpose

    Localization: Microtubules, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Tubulin (TUBA1B Human)
    

    Tags

    mOrange2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

16. gCOTS-pyl

    Plasmid

    #92048

    Purpose

    This is a S. elongatus (PCC7942) shuttle vector used for recombination of the pylRS orthogonal translation system to genomic neutral site 2.

    Depositor

    Lital Alfonta

    Article

    Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/

    Inserts

    Pyrrolysyl tRNA(cua)
    Pyrrolysyl tRNA synthetase (methanosarcina mazei)
    

    Use

    Cyanobacteria s. elongatus genome recombination i…

    Promoter

    LeuP (native S. elongatus promoter) and PrcbL

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEB2-mRFP1*

    Plasmid

    #104000

    Purpose

    Plasmid encoding mRFP1*

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mRFP1*
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to DsRed (MSKGEE NNLAVIKEF...T…

    Promoter

    proC

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV/MASC_(GLuc)_INT

    Plasmid

    #68440

    Purpose

    Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/ MASC expression backbone.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing three PP7 Stem-loops
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLQ2804 pHR: U6-SpsgTRE3G CMV-PYL1-VPR-IRES-mCherry

    Plasmid

    #84258

    Purpose

    Expresses Sp sgTRE3G gRNA with ABA-inducible VPR and mCherry for OR gate

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    Sp sgTRE3G
    PYL1-VPR
    

    Use

    CRISPR and Lentiviral

    Tags

    IRES-mCherry and PYL1

    Expression

    Mammalian

    Promoter

    CMV and mouse U6

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. mRuby2-PDHA1-N-10

    Plasmid

    #55909

    Purpose

    Localization: Mitochondria, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    PDHA1 (PDHA1 Human)
    

    Tags

    mRuby2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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