We narrowed to 9,627 results for: otos

1. (302) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5)

   Plasmid

   #168192

   Purpose

   HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGT of 4 TPRs

   Depositor

   Christina Woo

   Article

   Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

   Insert

   HA-LaG6-(EAAAK)4-OGT(4)
   

   Use

   Tags

   HA-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJB158

   Plasmid

   #202601

   Purpose

   RP4-based mobilizable plasmid for the IPTG inducible expression of inserts that can be introduced using BsaI-based Golden Gate cloning. Vector has two origins of replication.

   Depositor

   Athanasios Typas

   Article

   Bobonis et al Nat Protoc. 2024 May 9. doi: 10.1038/s41

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3 CDK7 D155A HA (P#812)

   Plasmid

   #14648

   Depositor

   Matija Peterlin

   Article

   Kanazawa et al Oncogene. 2003 Aug 28. 22(36):5707-11.

   Insert

   Cdk7 (CDK7 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D155A: dominant negative

   Promoter

   Available since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

4. mEos4b-Lifeact-7

   Plasmid

   #54813

   Purpose

   Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Loren Looger, Michael Davidson

   Article

   Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Type

   Empty backbone

   Use

   Tags

   mEos4b-Lifeact

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pET-BoNT/E

   Plasmid

   #168020

   Purpose

   Expresses BoNT/E-LC(1-411)-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Blum et al Science. 2021 Feb 19;371(6531):803-810.

   Insert

   BoNT/E-LC(1-411)-6xHis
   

   Use

   Tags

   Hisx6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKin1B

   Plasmid

   #31604

   Depositor

   Anthony Brown

   Article

   Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

   Insert

   kinesin-1B (Kif5b Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTP924 pHAGE2 CMV GFP

   Plasmid

   #199392

   Purpose

   Lentiviral transfer plasmid for constitutive expression of a GFP in mammalian cells

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRDV5 containing Sb-convex

   Plasmid

   #132696

   Purpose

   pRDV5 plasmid containing a convex Sybody for the generation of a qPCR standard curve

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

   Insert

   Sb_convex including 5' and 3' flanking sequences used for ribosome display
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKLV-EF1aGFP-W

   Plasmid

   #159291

   Purpose

   Lentiviral vector expressing GFP driven by human EF1a promoter

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human EF1a promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS-H2B-citrine

    Plasmid

    #53752

    Purpose

    Contains fusion of human Histone H2B to citrine.

    Depositor

    Sean Megason

    Article

    Megason et al Methods Mol Biol. 2009;546:317-32. doi:

    Insert

    histone H2B (H2BC21 Human)
    

    Use

    Tags

    citrine

    Expression

    Mammalian

    Mutation

    Promoter

    simian CMV IE94

    Available since

    July 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUB-cHyPer2

    Plasmid

    #84728

    Purpose

    Expresses HyPer2 in plant cells-biosensor for Hydrogen peroxide

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Insert

    HyPer2
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTS103 pHAGE2 CMVtetO2 MCS-22xGS-GFP

    Plasmid

    #199356

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally GFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBiex-1 Myosin VI HMM_SNAP

    Plasmid

    #82716

    Purpose

    Expresses Myosin VI HMM with a C-terminal SNAP tag.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Hariadi et al Nat Nanotechnol. 2015 Aug;10(8):696-700.

    Insert

    Myosin VI
    

    Use

    Tags

    6xHis Tag, FLAG purification tag, and SNAP tag

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    T7 Promoter

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSETb-ffDronpa

    Plasmid

    #78182

    Purpose

    Expresses ffDronpa E. coli strains

    Depositor

    Peter Dedecker

    Article

    Moeyaert et al ACS Nano. 2014 Feb 25;8(2):1664-73. doi:

    Insert

    ffDronpa
    

    Use

    Tags

    6xHis-tag; Xpress tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTorPE-G-GECO1

    Plasmid

    #32466

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    G-GECO1.0
    

    Use

    Lab constructed

    Tags

    6 His tag and Modified TorA MNNNDLFQASRRRFQAQLGGL…

    Expression

    Bacterial

    Mutation

    GCaMP3 K119I/L173Q/S404G/E430V

    Promoter

    Available since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSN444

    Plasmid

    #108331

    Purpose

    Expresses Sat2 for Nourseothricin resistance selection of C. elegans

    Depositor

    Shinsuke Niwa

    Article

    Obinata et al PLoS One. 2018 May 9;13(5):e0197128. doi

    Insert

    Streptothricin acetyl transferase 2
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rps-27 promoter

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. 3xFLAG-dCas9/pTEF1p-CYC1t

    Plasmid

    #62190

    Purpose

    Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujii et al Biol Methods Protoc. 2022 Oct 17;7(1):bp

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Yeast

    Mutation

    human codon-optimized, D10A, H840A

    Promoter

    TEF1 promoter

    Available since

    Feb. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. tol2-CAG::ChR2-YFP

    Plasmid

    #59740

    Purpose

    Optogenetic excitation of ES cell-derived neurons (and other cells, such as muscle)

    Depositor

    Ivo Lieberam

    Article

    Bryson et al Science. 2014 Apr 4;344(6179):94-7. doi:

    Insert

    Channelrhodopsin-2
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    CAG

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

19. Teto Ngn1

    Plasmid

    #62222

    Purpose

    Inducible expression of Ngn1

    Depositor

    Kristin Baldwin

    Article

    Blanchard et al Nat Neurosci. 2015 Jan;18(1):25-35. doi:

    Insert

    Neurog1 (Neurog1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-Bmp2

    Plasmid

    #163587

    Purpose

    Expresses BMP-2 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmp2 (Bmp2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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