We narrowed to 4,212 results for: Pet2

1. pET28HistevTtTERT(1-516)

   Plasmid

   #28171

   Depositor

   Kathleen Collins

   Article

   O'Connor et al J Biol Chem. 2005 Apr 29. 280(17):17533-

   Insert

   Tetrahymena TERT
   

   Use

   Tags

   6xhistidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28_Caspase-4_p20_C258A

   Plasmid

   #214307

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   CASP4 (CASP4 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   C258A

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET21b-P35

   Plasmid

   #11808

   Depositor

   Guy Salvesen

   Article

   Riedl et al Biochemistry. 2001 Nov 6. 40(44):13274-8

   Insert

   P35
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pET28a-MSP1D1delltaH4

   Plasmid

   #71713

   Purpose

   Membrane scaffold protein (MSP), helix 4 deletion

   Depositor

   Gerhard Wagner

   Article

   Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

   Insert

   Apolipoprotein A1 (APOA1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a-AncMEcoGII250

   Plasmid

   #205025

   Purpose

   Expressing engineered M.EcoGII with higher thermostability

   Depositor

   Paola Laurino

   Article

   Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

   Insert

   Ancestral M.EcoGII 250
   

   Use

   Tags

   His6x tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a-SpySrtA

   Plasmid

   #51139

   Purpose

   expression plasmid for N-terminal his tagged S.pyogenes SrtA

   Depositor

   Hidde Ploegh

   Article

   Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

   Insert

   S.pyogenes SrtA
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-80

   Promoter

   T7

   Available since

   Feb. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-mCherryGGBsMTS

   Plasmid

   #133625

   Purpose

   Expression vector for mCherry fused to a membrane targeting sequence (MTS) of the B.subtilis MinD (C-terminal fusion) and an N-terminal His-tag for purification.

   Depositor

   Petra Schwille

   Article

   Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

   Insert

   mCherryGGBsMTS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET21a.Tat(48-57)-EGFP

   Plasmid

   #165040

   Purpose

   Expression of Tat(48-57) conjugated EGFP for protein purification (6XHis tag)

   Depositor

   Dokyun Na

   Article

   Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

   Insert

   EGFP
   

   Use

   Tags

   6XHis and Tat(48-57)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-PHGDH

   Plasmid

   #74113

   Purpose

   Expression of N-terminal His-tagged

   Depositor

   Lewis Cantley

   Article

   Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

   Insert

   PHGDH (PHGDH Human)
   

   Use

   Tags

   His-tagged

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET24c(+)-EFTu

    Plasmid

    #31172

    Depositor

    Linda Spremulli

    Article

    Zhang et al FEBS Lett. 1996 Aug 12. 391(3):330-2.

    Insert

    E. coli elongation factor Tu (tufB E. coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pET28a-PFO

    Plasmid

    #92109

    Purpose

    Recombinant expression in E.Coli

    Depositor

    Feng Shao

    Article

    Ding et al Nature. 2016 Jul 7;535(7610):111-6. Epub

    Insert

    PFO
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Delta27aa

    Promoter

    Available since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a_T5-ARG1

    Plasmid

    #106476

    Purpose

    Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917

    Depositor

    Mikhail Shapiro

    Article

    Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

    Insert

    ARG1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a(+)- PC-MjMAT

    Plasmid

    #179372

    Purpose

    PC-MjMAT is an engineered methionine adenosyltransferase that catalyses the chemo-enzymatic generation of AdoMet analogs carrying photocaging groups.

    Depositor

    Andrea Rentmeister

    Article

    Peters et al Chembiochem. 2021 Oct 4. doi: 10.1002/cb

    Insert

    PC-MjMAT
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    L147A, I351A

    Promoter

    T7

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET24a MDH_AQUAR

    Plasmid

    #204239

    Purpose

    Bacterial expression of A. arcticum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDH_AQUAR
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET23a-mUbc9

    Plasmid

    #53137

    Purpose

    E.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)

    Depositor

    Frauke Melchior

    Article

    Pichler et al Cell. 2002 Jan 11;108(1):109-20.

    Insert

    Ubc9 (Ube2i Mouse)
    

    Use

    Tags

    untagged

    Expression

    Bacterial

    Mutation

    protein sequence is identical to human

    Promoter

    T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET24a MDH_9CREN

    Plasmid

    #204243

    Purpose

    Bacterial expression of I. islandicus malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDH_9CREN
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a MDHC_YEAST

    Plasmid

    #204232

    Purpose

    Bacterial expression of yeast cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDHC_YEAST (MDH2 Budding Yeast)
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28_Caspase-4_K356D

    Plasmid

    #214312

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (CASP4 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    K356D

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a Gx-mNG-NL

    Plasmid

    #169106

    Purpose

    Expresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - mNeonGreen - NanoLuc
    

    Use

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET24c(+)-EFTs

    Plasmid

    #31173

    Depositor

    Linda Spremulli

    Article

    Zhang et al FEBS Lett. 1996 Aug 12. 391(3):330-2.

    Insert

    E. coli elongation factor Ts
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only