Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 587 results for: F12

Clear keywords
Showing: 511 - 540 of 587 results

  3. GST-CHMP4A (205-222) E209A (pGEX2T(TEV))

    Plasmid
    #80640
    Purpose
    Residues 205-222 of CHMP4A, Mutation modestly disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-206
    Depositor
    Wesley Sundquist
    Article
    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105
    Insert
    CHMP4A (CHMP4A Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Changed Glutamate 209 to alanine
    Available Since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    80640-plasmid-map-sequence-id-161707

    Enlarge

  9. pCA528 CHMP4A 153-222

    Plasmid
    #108256
    Purpose
    Expresses C-terminal 153-222 residues of CHMP4A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-6
    Depositor
    Wesley Sundquist
    Article
    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75
    Insert
    CHMP4A residues 153-222 (CHMP4A Human)
    Tags
    His-SUMO
    Expression
    Bacterial
    Mutation
    Contains nonnative Gly-Cys for fluorescent labeli…
    Promoter
    T7
    Available Since
    Nov. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    108256-plasmid-map-sequence-id-245257

    Enlarge

  10. TFORF1311

    Plasmid
    #143786
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    RBMS1 (RBMS1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143786-plasmid-map-sequence-id-396236

    Enlarge

  11. GST-CHMP4A (205-222) L217A (pGEX2T(TEV))

    Plasmid
    #80638
    Purpose
    Residues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-204
    Depositor
    Wesley Sundquist
    Article
    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105
    Insert
    CHMP4A (CHMP4A Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Residues 205-222; Changed Leucine 217 to Alanine
    Available Since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    80638-plasmid-map-sequence-id-161738

    Enlarge

  12. GST-CHMP4A (205-222) W220A (pGEX2T(TEV))

    Plasmid
    #80637
    Purpose
    Residues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-203
    Depositor
    Wesley Sundquist
    Article
    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105
    Insert
    CHMP4A (CHMP4A Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Residues 205-222; Changed Trp 220 to Ala
    Available Since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    80637-plasmid-map-sequence-id-161733

    Enlarge

  13. GST-CHMP4A (205-222) L214A (pGEX2T(TEV))

    Plasmid
    #80639
    Purpose
    Residues 205-222 of CHMP4A, Mutation disrupts binding to ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist Lab Internal ID: WISP06-205
    Depositor
    Wesley Sundquist
    Article
    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105
    Insert
    CHMP4A (CHMP4A Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Residues 205-222; Changed Leucine 214 to Alanine
    Available Since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    80639-plasmid-map-sequence-id-161725

    Enlarge

  19. TFORF3506

    Plasmid
    #144982
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    LCOR (LCOR Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    144982-plasmid-map-sequence-id-391563

    Enlarge

  22. TFORF2132

    Plasmid
    #141935
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    LCOR (LCOR Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141935-plasmid-map-sequence-id-415086

    Enlarge

  30. Venus-Bad-4E-pEGFP-C1

    Plasmid
    #166740
    Purpose
    Express Venus fused to the N-terminus of the Bcl-2 family protein, Bad with mutation in BH3 domain to disrupt binding anti-apoptotic proteins
    Depositor
    David Andrews
    Article
    Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1
    Insert
    Bad-4E (BAD Human)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    4 hydrophobic residues in the BH3 region mutated …
    Promoter
    CMV
    Available Since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166740-plasmid-map-sequence-id-329000

    Enlarge

Showing: 511 - 540 of 587 results