We narrowed to 1,410 results for: puc57

1. dg263_pENTR_ChrimsonCel

   Plasmid

   #66101

   Purpose

   Entry clone containing Chrimson coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2

   Depositor

   Dominique Glauser

   Article

   Schild et al Genetics. 2015 May 28. pii: genetics.115

   Insert

   Chrimson
   

   Use

   Gateway

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pToneTP

   Plasmid

   #67692

   Purpose

   For in vitro transcription of mRNA encoding Tol1 Transposase

   Depositor

   Nathan Lawson

   Article

   Shin et al Development. 2016 Oct 15;143(20):3785-37

   Insert

   Tol1 transposase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMLS134

   Plasmid

   #73714

   Purpose

   SapTrap destination vector for building sgRNA expression vectors.

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   pU6::sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   SapI insertion site

   Promoter

   C. elegans U6 snRNA pol III promoter

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMPRAdonor1

   Plasmid

   #49352

   Purpose

   Promoterless luc2 donor plasmid for MPRA library construction

   Depositor

   Tarjei Mikkelsen

   Article

   Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

   Insert

   luc2
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. MAPT_Halo_C_allele

   Plasmid

   #178143

   Purpose

   Donor vector for endogenous tagging of human MAPT at the C-terminus with halotag

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Halotag (MAPT Human)
   

   Use

   Donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMPRAdonor3

   Plasmid

   #49354

   Purpose

   pSV40mod-luc2 donor plasmid for MPRA library construction

   Depositor

   Tarjei Mikkelsen

   Article

   Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

   Insert

   luc2
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   SV40mod

   Available since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. dg265_pENTR_CoChRCel

   Plasmid

   #66103

   Purpose

   Entry clone containing CoChR coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2

   Depositor

   Dominique Glauser

   Article

   Schild et al Genetics. 2015 May 28. pii: genetics.115

   Insert

   CoChR
   

   Use

   Gateway

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. HNRNPA1_Halo_C_allele

   Plasmid

   #178123

   Purpose

   Donor vector for endogenous tagging of human HNRNPA1 at the C-terminus with halotag

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Halotag (HNRNPA1 Human)
   

   Use

   Donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ADAM10_Halo_C_allele

   Plasmid

   #178151

   Purpose

   Donor vector for endogenous tagging of human ADAM10 at the C-terminus with halotag

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Halotag (ADAM10 Human)
   

   Use

   Donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FUS_Halo_N_allele_400

    Plasmid

    #161767

    Purpose

    Donor vector for endogenous tagging of human FUS at the N-terminus with halotag

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    halotag (FUS Human)
    

    Use

    Donor vector for endogenous tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pIK86

    Plasmid

    #47933

    Purpose

    C. elegans codon-optimized Cas9 with 2xSV40 NLS sequences. It has a T7 promoter for in vitro transcription, germline-compatible 5'UTR, tbb-2 3'UTR and a polyA sequence.

    Depositor

    Iskra Katic

    Article

    Katic et al Genetics. 2013 Aug 26.

    Insert

    Cas9
    

    Use

    CRISPR and Unspecified

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. APP_Halo_C_allele

    Plasmid

    #178137

    Purpose

    Donor vector for endogenous tagging of human APP at the C-terminus with halotag

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Halotag (APP Human)
    

    Use

    Donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FUS_Halo_C_allele_400

    Plasmid

    #161768

    Purpose

    Donor vector for endogenous tagging of human FUS at the C-terminus with halotag

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    halotag (FUS Human)
    

    Use

    Donor vector for endogenous tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTK398

    Plasmid

    #114714

    Purpose

    mAID-mClover-3FLAG tagging at NuMA's C-terminal locus

    Depositor

    Tomomi Kiyomitsu

    Article

    Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

    Insert

    mAID-mClover-3FLAG
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. p214_LTJ_1kbCD90.1Template

    Plasmid

    #82667

    Purpose

    1kb HDR template for murine CD90.1

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    1kb genomic DNA sequence from murine CD90.1
    

    Use

    Hdr template for gene editing

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p229_LTJ_2kbCD90.1Template

    Plasmid

    #82668

    Purpose

    2kb HDR template for murine CD90.1

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    2kb genomic DNA sequence from murine CD90.1
    

    Use

    Hdr template for gene editing

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p248_LTJ_2kbCD45.1Template

    Plasmid

    #82662

    Purpose

    2kb HDR template for murine CD45.1

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    2kb genomic DNA sequence from murine CD45.1
    

    Use

    Hdr template for gene editing

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p213_LTJ_160bpCD90.1Template

    Plasmid

    #82666

    Purpose

    160bp HDR template for murine CD90.1

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    160bp genomic DNA sequence from murine CD90.1
    

    Use

    Hdr template for gene editing

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p230_LTJ_4kbCD90.1Template

    Plasmid

    #82669

    Purpose

    4kb HDR template for murine CD90.1

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    4kb genomic DNA sequence from murine CD90.1
    

    Use

    Hdr template for gene editing

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p242_LTJ_1kbCD45.1Template

    Plasmid

    #82661

    Purpose

    1kb HDR template for murine CD45.1

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    1kb genomic DNA sequence from murine CD45.1
    

    Use

    Hdr template for gene editing

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. dg264_pENTR_ChronosCel

    Plasmid

    #66102

    Purpose

    Entry clone containing Chronos coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2

    Depositor

    Dominique Glauser

    Article

    Schild et al Genetics. 2015 May 28. pii: genetics.115

    Insert

    Chronos
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CSF1R_Halo_C_allele

    Plasmid

    #178139

    Purpose

    Donor vector for endogenous tagging of human CSF1R at the C-terminus with halotag

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Halotag (CSF1R Human)
    

    Use

    Donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. AA516

    Plasmid

    #231118

    Purpose

    Fragmid fragment: (2A Selection) CBE activity-based selection intron

    Depositor

    John Doench, David Root

    Insert

    PuroN'-intron-PuroC'
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AA498

    Plasmid

    #231119

    Purpose

    Fragmid fragment: (guide cassette) ABE activity-based selection CD274 positive control

    Depositor

    John Doench, David Root

    Insert

    sgCD274 + ABE splice-targeting sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AA505

    Plasmid

    #231120

    Purpose

    Fragmid fragment: (guide cassette) CBE activity-based selection CD274 positive control

    Depositor

    John Doench, David Root

    Insert

    sgCD274 + CBE splice-targeting sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AA509

    Plasmid

    #231116

    Purpose

    Fragmid fragment: (2A Selection) ABE activity-based selection intron

    Depositor

    John Doench, David Root

    Insert

    PuroN'-intron-PuroC'
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSA_0_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10

    Plasmid

    #199487

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10

    Plasmid

    #199488

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSA_2_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10

    Plasmid

    #199489

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pKozak_Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10

    Plasmid

    #199497

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge