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LC101: pMVP/PB/Neo-DEST

Plasmid

#121875

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Neo selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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JN302: pMVP/SB/Puro-DEST

Plasmid

#121865

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Puro selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pIC292

Plasmid

#51405

Purpose

Retroviral expression of EGFP-TEV-S-tag SKA2 for protein localization and affinity purification (LAP)

Depositor

Iain Cheeseman

Article

Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

Insert

Ska2 IMAGE 4685972 (SKA2 Human)

Use

Retroviral

Tags

EGFP, S tag, and TEV protease site

Expression

Mammalian

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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C-term Hec1-targeted Aurora B FRET sensor

Plasmid

#45222

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

Insert

C-term Hec1-targeted Aurora B FRET sensor

Expression

Mammalian

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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PU.1-S148A LZRS

Plasmid

#44633

Depositor

Ellen Rothenberg

Article

Dionne et al Dev Biol. 2005 Apr 15;280(2):448-66.

Insert

PU.1 S148A (Spi1 Mouse)

Use

Retroviral

Tags

EGFP and IRES

Expression

Mammalian

Mutation

148 mutation (S to A)

Available Since

April 22, 2013

Availability

Academic Institutions and Nonprofits only
GFP KNL1 wt hardened and RVSF->AAAA

Plasmid

#45225

Depositor

Iain Cheeseman, Michael Lampson

Article

Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

Insert

GFP KNL1 with RVSF-AAAA mutation, resistant to KNL1 siRNA (KNL1 Human)

Mutation

RVSF mutated to AAAA

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only
GB1-GyrA

Plasmid

#128738

Purpose

Expresses GB1 fused to GyrA intein at the C-terminal

Depositor

Sharon Rozovsky

Article

Liu et al Chembiochem. 2019 Jul 2. doi: 10.1002/cb

Insert

GB1

Tags

GyrA

Expression

Bacterial

Promoter

T7

Available Since

Aug. 12, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pBigTB

Plasmid

#149432

Purpose

shuttle vector with blasticidin-S resistance into ROSA26 targetting vectors

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Type

Empty backbone

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pWPI-SLC7A11-HA

Plasmid

#201643

Purpose

Over-expression of HA-tagged human SLC7A11

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

SLC7A11 (SLC7A11 Human)

Use

Lentiviral

Tags

HA

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCK054 pDEST exorh:mCerulean

Plasmid

#195985

Purpose

Transgenesis backbone

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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LB701: pMVP/PB/mCherry-DEST

Plasmid

#121871

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ mCherry selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCDF-His-mTET1CD∆cat

Plasmid

#81054

Purpose

bacterial expression of catalytic dead catalytic domain of murine TET1

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TET1 (Tet1 Mouse)

Tags

6HIS

Expression

Bacterial

Mutation

catalytic domain amino acid 1367-2057 with histid…

Promoter

T7

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-GPD1

Plasmid

#32487

Depositor

Pere Puigserver

Article

Kelly et al Mol Cell Biol. 2011 Jul;31(13):2696-706.

Insert

GPD1 (Gpd1 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only
PCBP1_Full-length_pGEX

Plasmid

#135108

Purpose

Recombinant expression of PCBP1 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

PCBP1 (PCBP1 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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JC001: pMVP/Ad/RGD-DEST

Plasmid

#121844

Purpose

pMVP destination vector, empty RGD fiber-modified adenovirus vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Adenoviral; Pmvp gateway destination vector

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pID1072

Plasmid

#164869

Purpose

The pCYMR-1.phiKO2cB plasmid carrying the gene for phiKO2 cB repressor. Genomic integration vector for expression of any candidate DNA looping protein.

Depositor

Keith Shearwin

Article

Hao et al Nucleic Acids Res. 2021 Jan 28. pii: 612

Insert

phiKO2 cB repressor

Expression

Bacterial

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pBbS8k-RFP_E83K

Plasmid

#109412

Purpose

RepA Mutant

Depositor

Jay Keasling

Article

Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

Insert

repA

Tags

RFP

Expression

Bacterial

Mutation

E83K repA mutation to increase copy number

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pBoPyV3-S23

Plasmid

#62369

Purpose

Complete genome of BoPyV3 isolate S23

Depositor

Christopher Buck

Article

Peretti et al J Gen Virol. 2015 Jan 7. pii: vir.0.0000

Insert

Full genome of BoPyV3 isolate S23

Use

Viral clone

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pBoPyV3-S22

Plasmid

#62368

Purpose

Complete genome of BoPyV3 isolate S22

Depositor

Christopher Buck

Article

Peretti et al J Gen Virol. 2015 Jan 7. pii: vir.0.0000

Insert

Full genome of BoPyV3 isolate S22

Use

Viral clone

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pBoPyV2b-S24

Plasmid

#62357

Purpose

Complete genome of BoPyV2b isolate S24

Depositor

Christopher Buck

Article

Peretti et al J Gen Virol. 2015 Jan 7. pii: vir.0.0000

Insert

Full genome of BoPyV2b isolate S24

Use

Viral clone

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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