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pZH1 Dual G-less cassette

Plasmid

#231645

Purpose

plasmid containing dual G-less cassette for in vitro transcription

Depositor

Jesper Svejstrup

Article

Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pRSET-WHaloCaMP-eNOSpep-EGFP

Plasmid

#205306

Purpose

Expression of WHaloCaMP-eNOSpep-EGFP fusion in E. coli

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP-eNOSpep-EGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti_CMVTRE3G_LYSET-Isoform2-I142V-Flag

Plasmid

#192006

Purpose

Lentiviral vector to generate flag-tagged LYSET(TMEM251)-isoform2 I142V mutant stable expressing cell line under CMVTRE3G promoter

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-Isoform2-I142V-Flag (LYSET Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMVTRE3G

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_CMVTRE3G_LYSET-Isoform2-R45W-Flag

Plasmid

#192005

Purpose

Lentiviral vector to generate flag-tagged LYSET(TMEM251)-isoform2 R45W mutant stable expressing cell line under CMVTRE3G promoter

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-Isoform2-R45W (LYSET Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMVTRE3G

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+-dCas9-olEzh2(deltaSET)

Plasmid

#180217

Purpose

Ezh2 (delta SET domain) fused to dCas9 for H3K27me3 epigenome editing control.

Depositor

Hiroyuki Takeda

Article

Fukushima et al Epigenetics Chromatin. 2019 Mar 13;12(1)

Insert

enhancer of zeste 2 polycomb repressive complex 2 subunit (deltaSET)

Use

Tags

FLAG and dCas9

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pRSET-QUEEN 7mu R126A

Plasmid

#129376

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN 7mu R126A

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-QUEEN 7mu R126I

Plasmid

#129377

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN 7mu R126I

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-QUEEN 7mu A81I

Plasmid

#129352

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN 7mu A81I

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-QUEEN 7mu D89A

Plasmid

#129354

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN 7mu D89A

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-QUEEN 7mu V90D

Plasmid

#129355

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN 7mu V90D

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-QUEEN 7mu A81E

Plasmid

#129356

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN 7mu A81E

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-QUEEN 7mu I88D

Plasmid

#129357

Purpose

mutant of QUEEN-7mu

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN 7mu I88D

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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enz.043-SETD7-Y305F

Plasmid

#154045

Purpose

Expresses SETD7 (Y305F) in E. coli

Depositor

Christopher Voigt

Article

Wallace et al Advanced Functional Materials 2020

Insert

Histone-lysine N-methyltransferase SETD7 (SETD7 Human)

Use

Tags

His, MBP

Expression

Bacterial

Mutation

changed tyrosine 305 to phenylalanine

Promoter

T7

Available since

Availability

Academic Institutions and Nonprofits only

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pBad-Xis/Int Reset Flipper

Plasmid

#38207

Depositor

Drew Endy

Article

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Inserts

Bxb1-Excisionase
Bxb1 Integrase

Use

Synthetic Biology; Biobrick cloning vector

Tags

Expression

Mutation

Promoter

pBAD

Available since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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Nano-lantern(cAMP-1.6)/pRSETB

Plasmid

#53591

Purpose

Nano-lantern-based luminescent cAMP indicator

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern-based luminescent cAMP indicator

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
PGK+ plasmid with onboard mCherry cassette

Plasmid

#169745

Purpose

PGK+ plasmid that also encodes separate mCherry cassette. mCherry is used to monitor plasmid dosage.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

eGFP

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

PGK-tetO2

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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PRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9

Plasmid

#186699

Purpose

Plasmid encoding PRDM9dC-Cas9 fusion under CMV promoter

Depositor

Jennifer Doudna

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

Insert

PRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9 (PRDM9 Human)

Use

CRISPR

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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AAVS1 KanR foundation lox casette

Plasmid

#132388

Purpose

Foundation cassette containing lox sites for cre-mediated exchange of inducible vectors, confers mammalian G418 resistance, targets AAVS1 locus

Depositor

Gerold Schmitt-Ulms

Article

Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

Insert

G418 resistance

Use

CRISPR and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

promoterless

Available since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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Equalizer-H plasmid with onboard mCherry cassette

Plasmid

#169733

Purpose

Equalizer-H plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

tetR-P2A-eGFP-miR(FF3) target-miR(FF3)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV-tetO2

Available since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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hSyn-GW-dCas9-SET2-IRES2-mCherry

Plasmid

#202089

Purpose

Neuron-specific expression vector of dCas9-Set2

Depositor

Elizabeth Heller

Article

Xu et al Neuron. 2021 Sep 15;109(18):2943-2966.e8

Insert

dCas9-Set2

Use

Tags

3x FLAG and mCherry

Expression

Mammalian

Mutation

Promoter

human Synapsin

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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