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We narrowed to 13,524 results for: TIM

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human EB1-GFP mutagenized S16 R17 H18 D19 W23 N25 all converted to A (JB139)

Plasmid

#39300

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S16A, R17A, H18A, D19A, W23A and N25A

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
human EB1 in pBluescript II

Plasmid

#39324

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

T7

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
human EB1-GFP mutagenized to remove terminal 7 aa (QEEQEEY) (JB162))

Plasmid

#39308

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

C-terminal 7 aa (QEEQEEY) removed

Promoter

CMV

Available since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only
human EB1-GFP mutagenized Y44 Q46 both converted to A (JB140)

Plasmid

#39301

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Y44A and Q46A

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
human EB1-GFP mutagenized D88 K89 D94 K95 all converted to A (JB141)

Plasmid

#39302

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

D88A, K89A, D94A and K95A

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
human EB1-GFP mutagenized Q109 W110 K112 K113 all converted to A (JB142)

Plasmid

#39303

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Q109A, W110A, K112A and K113A

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
human EB1-GFP mutagenized K122 D123 Y124 D125 all converted to A (JB143)

Plasmid

#39304

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

K122A, D123A, Y124A and D125A

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
human EB1-GFP mutagenized K148 K150 K151 all converted to A (JB144)

Plasmid

#39305

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

K148A, K150A and K151A

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
human EB1-GFP mutagenized E211 K212 E213 R214 D215 Y217 all converted to A (JB145)

Plasmid

#39306

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

E211A, K212A, E213A, R214A, D215A and Y217A

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
human EB1-GFP mutagenized T249 D250 E251 all converted to A (JB146)

Plasmid

#39307

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

T249A, D250A and E251A

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pAAV.hSynapsin.(cyto).cpSFGFP.HaloTag

Plasmid

#209666

Purpose

Non-responsive control

Depositor

HHMI-JRC Tool Translation Team, Tim Brown

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

cpSFGFP.HaloTag

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CAG.(cyto).iATPSnFR2.S29W.A95K.HaloTag

Plasmid

#209651

Purpose

Expression of iATPSnFR2, high affinity

Depositor

HHMI-JRC Tool Translation Team, Tim Brown

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.S29W.HaloTag

Use

AAV

Tags

Expression

Mutation

S29W.A95K

Promoter

CAG

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV.CAGFLEX.(cyto).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209657

Purpose

Expression of iATPSnFR2, low affinity

Depositor

HHMI-JRC Tool Translation Team, Tim Brown

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.HaloTag

Use

AAV

Tags

Expression

Mutation

A95A.A119L

Promoter

CAG

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.hSynapsin.(cyto).iATPSnFR2.A95K.HaloTag

Plasmid

#209664

Purpose

Expression of iATPSnFR2, medium affinity

Depositor

HHMI-JRC Tool Translation Team, Tim Brown

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.HaloTag

Use

AAV

Tags

Expression

Mutation

A95K

Promoter

hSyn

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209653

Purpose

Expression of iATPSnFR2, low affinity

Depositor

HHMI-JRC Tool Translation Team, Tim Brown

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.HaloTag

Use

AAV

Tags

Expression

Mutation

A95A.A119L

Promoter

CAG

Available since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CAG.(cyto).iATPSnFR2.A95K.HaloTag

Plasmid

#209652

Purpose

Expression of iATPSnFR2, medium affinity

Depositor

HHMI-JRC Tool Translation Team, Tim Brown

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.HaloTag

Use

AAV

Tags

Expression

Mutation

A95K

Promoter

CAG

Available since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CAG.(cyto).cpSFGFP.HaloTag

Plasmid

#209654

Purpose

Non-responsive control

Depositor

HHMI-JRC Tool Translation Team, Tim Brown

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

cpSFGFP.HaloTag

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.hSynapsin.(cyto).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209665

Purpose

Expression of iATPSnFR2, low affinity

Depositor

HHMI-JRC Tool Translation Team, Tim Brown

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.HaloTag

Use

AAV

Tags

Expression

Mutation

A95A.A119L

Promoter

hSyn

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSET.HaloTag.v7.AviTag

Plasmid

#195486

Purpose

Bacterial expression plasmid with Halotag v7 insert plus a C-terminus appended AviTag for enzymatic biotinylation.

Depositor

HHMI-JRC Tool Translation Team, Tim Brown

Insert

HaloTag.v7.AviTag

Use

Tags

6xHIS and AviTag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CAGFLEX.(cyto).cpSFGFP.HaloTag

Plasmid

#209658

Purpose

Non-responsive control

Depositor

HHMI-JRC Tool Translation Team, Tim Brown

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

cpSFGFP.HaloTag

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge