We narrowed to 4,332 results for: U6 gRNA

1. pENTR223-CMV-Cre-U6-sgKdm6a#4-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

   Plasmid

   #209061

   Purpose

   Entry vector that endcodes sgRNAs against mouse Kdm6a, Rb1, Trp53, and Rbl2 and CMV Cre recombinase.

   Depositor

   Matthew Oser

   Article

   Duplaquet et al Nat Cell Biol. 2023 Sep;25(9):1346-1358.

   Insert

   KDM6A
   

   Use

   Gateway vector to be used for lr reaction

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR U6-hTERTsh

   Plasmid

   #111385

   Purpose

   Knock down of hTERT transcript

   Depositor

   Klaus Holzmann

   Article

   Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

   Insert

   shRNA C3 against hTERT
   

   Use

   Gateway entry cloning

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZac2.1 EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

   Plasmid

   #78607

   Purpose

   A single vector AAV-Cas9 system containing SaCas9 under EFs promoter, gRNAs targeting Dmd introns 22 and 23

   Depositor

   Amy Wagers

   Article

   Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

   Insert

   EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2
   

   Use

   AAV and CRISPR

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-short; U6

   Available since

   Sept. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

4. pXJ218-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-mtagBFP2-KASH-PB5'IR

   Plasmid

   #213480

   Purpose

   AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.

   Depositor

   Xin Jin

   Article

   Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

   Inserts

   mtagBFP-KASH
   U6-gRNA-directcapture
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pXJ197-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-GFP-KASH-PB5'IR

   Plasmid

   #210523

   Purpose

   AAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.

   Depositor

   Xin Jin

   Article

   Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

   Inserts

   GFP-KASH
   U6-gRNA-directcapture
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-MYL2-HA-TadA8e-Sauri N aa1-438-inteinN-U6-Camk2d sgRNA7

   Plasmid

   #226678

   Purpose

   Expresses TadA8e and Sauri cas9N by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

   Depositor

   Yuxuan Guo

   Article

   Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

   Insert

   MYL2, TadA, nSauri Cas9N, inteinN
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   MYL2

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCFD4d-U6-1:white1-U6-3:white1

   Plasmid

   #84005

   Purpose

   pCFD4d expresses white sgRNA-1 from a U6:3 promoter and the same white sgRNA-1 from a U6:1 promoter

   Depositor

   Phillip Zamore

   Article

   Ge et al G3 (Bethesda). 2016 Aug 26. pii: g3.116.

   Inserts

   white sgRNA-1
   white sgRNA-1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6:1, U6:3, and dU6-1; dU6-3

   Available since

   Jan. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA

   Plasmid

   #61593

   Purpose

   A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.

   Depositor

   Feng Zhang

   Article

   Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

   Inserts

   hSaCas9
   Chimeric guide for SaCas9
   

   Use

   AAV and CRISPR

   Tags

   HA, NLS, and OLLAS tag

   Expression

   Mammalian

   Mutation

   K175R and K736R (deUb mutations)

   Promoter

   Available since

   Feb. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

   Plasmid

   #60231

   Purpose

   Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Cre recombinase
   EGFP
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA-P2A and EGFP-KASH

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and hSyn

   Available since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid

    #60230

    Purpose

    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    EGFP
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA-P2A and EGFP-KASH

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and hSyn

    Available since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAVS1-tdTomato targeting vector (compatible with CAGGS-AsCpf1-2A-GFP-U6-AAVS1-sgRNA)

    Plasmid

    #194728

    Purpose

    AAVS1-tdTomato targeting vector, compartible with Cpf1

    Depositor

    Rudolf Jaenisch

    Article

    Haiting et al bioRxiv

    Insert

    tdTomato
    

    Use

    CRISPR; Knockin targeting plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBS/U6/hTID1

    Plasmid

    #13745

    Depositor

    Karl Munger

    Article

    Edwards et al Oncogene. 2004 Nov 4. 23(52):8419-31.

    Insert

    hTid1 siRNA (DNAJA3 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Inverted repeat of 21 bp with 6 nuc. spacer.

    Promoter

    Available since

    March 5, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pAAV2ss-U6-sgGFP

    Plasmid

    #190899

    Purpose

    AAV vector expressing sgGFP

    Depositor

    Nicole Déglon

    Article

    Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

    Insert

    sgRNA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX601-AAV-CMV::NLS-saCas9-NLS-3xHA-bGHpa;U6::BsaI-sgRNA-lacZ

    Plasmid

    #175175

    Purpose

    A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting lacZ

    Depositor

    Bernardo Sabatini

    Article

    Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

    Insert

    sgRNA-lacZ
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Adrb1

    Plasmid

    #184294

    Purpose

    A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Adrb1

    Depositor

    Bernardo Sabatini

    Insert

    sgRNA-Adrb1
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Adra1b

    Plasmid

    #184293

    Purpose

    A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Adra1b

    Depositor

    Bernardo Sabatini

    Inserts

    sgRNA-Adra1b
    sgRNA-Adra1b
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX601-AAV-CMV::NLS-saCas9-NLS-3xHA-bGHpa;U6::BsaI-sgRNA-Grpr1

    Plasmid

    #175176

    Purpose

    A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Grpr

    Depositor

    Bernardo Sabatini

    Article

    Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

    Insert

    sgRNA-Grpr
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Grp

    Plasmid

    #184292

    Purpose

    A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Grp

    Depositor

    Bernardo Sabatini

    Insert

    sgRNA-Grp
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Oprl1

    Plasmid

    #184291

    Purpose

    A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Oprl1

    Depositor

    Bernardo Sabatini

    Insert

    sgRNA-Oprl1
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Rxfp3

    Plasmid

    #184290

    Purpose

    A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Rxfp3

    Depositor

    Bernardo Sabatini

    Insert

    sgRNA-Rxfp3
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge