We narrowed to 1,478 results for: asl;

1. pCellulose

   Plasmid

   #36300

   Depositor

   Jay Keasling

   Article

   Bokinsky et al Proc Natl Acad Sci U S A. 2011 Dec 13;10

   Insert

   pCellulose
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Two promoters from the E coli genome drive two di…

   Available since

   Nov. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKS18

   Plasmid

   #24629

   Depositor

   Jay Keasling

   Article

   Steen et al Nature. 2010 Jan 28;463(7280):559-62.

   Insert

   mFar1 (Far1 Mouse)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 11, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. JBEI-3716

   Plasmid

   #100169

   Purpose

   Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

   Depositor

   Jay Keasling

   Article

   Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

   Insert

   codon-optimized MK
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pXylan

   Plasmid

   #36301

   Depositor

   Jay Keasling

   Article

   Bokinsky et al Proc Natl Acad Sci U S A. 2011 Dec 13;10

   Insert

   pXylan
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Two promoters from the E coli genome drive two di…

   Available since

   Nov. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28b-hTIG3-1-164

   Plasmid

   #100724

   Purpose

   Full-length human TIG3 in E.coli expression vector

   Depositor

   Byung Il Lee

   Article

   Han et al Protein Expr Purif. 2010 May;71(1):103-7

   Insert

   RARRES3 (PLAAT4 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promotor

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28b-hTIG3-1-132

   Plasmid

   #100720

   Purpose

   Expression of N-terminal region of human TIG3 in E.coli

   Depositor

   Byung Il Lee

   Article

   Han et al Protein Expr Purif. 2010 May;71(1):103-7

   Insert

   RARRES3 (PLAAT4 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promotor

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPRKCZ-C1-EGFP (C-PRKCZ-C1)

   Plasmid

   #217759

   Purpose

   Fluorescent reporter for ceramide (putative, mammalian expression)

   Depositor

   Paula Nunes-Hasler

   Article

   Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

   Insert

   Protein kinase C zeta (PRKCZ Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C1 domain (aa 123-193)

   Promoter

   CMV

   Available since

   June 11, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. CRY2olig-mCh-CLC

   Plasmid

   #60035

   Purpose

   Expresses a fusion of CRY2olig (PHR, E490G) with mCherry and mouse clathrin light chain

   Depositor

   Chandra Tucker

   Article

   Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

   Insert

   CRY2-mCh-Clathrin Light Chain (CRY2 Mouse, Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   M354I, E490G CRY2, A9D*

   Promoter

   CMV

   Available since

   Oct. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR1a-tagRFP-KDEL

   Plasmid

   #114177

   Purpose

   Gateway entry clone containing tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ER

   Depositor

   Nicolas Demaurex

   Article

   Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

   Insert

   tagRFP-KDEL
   

   Use

   Gateway entry clone

   Tags

   KDEL retention signal and signal peptide

   Expression

   Mutation

   Promoter

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CRY2olig-mCh-Nck

    Plasmid

    #60034

    Purpose

    Expresses a fusion containing CRY2olig (PHR, E490G) with mCherry and 3 SH3 domains of Nck

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

    Insert

    CRY2-mCh-NCK (CRY2 Human, Mustard Weed)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    M354I, E490G CRY2

    Promoter

    CMV

    Available since

    Nov. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET19-M(BRCT)TdTwt

    Plasmid

    #207459

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domain

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    M(BRCT)TdTwt (Dntt Mouse)
    

    Use

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEGFP-PRKCZ-C1 (N-PRKCZ-C1)

    Plasmid

    #217760

    Purpose

    Fluorescent reporter for ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Protein kinase C zeta (PRKCZ Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C1 domain (aa 123-193)

    Promoter

    CMV

    Available since

    June 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pET19-TdTdelta5cys

    Plasmid

    #207465

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) surface-cysteine free variant

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    TdTdelta5cys (Dntt Mouse)
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Cys188Ala, Cys216Ser, Cys302Ala, Cys378Ala, and C…

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p2K7-bsd-UBI-tagRFP-KDEL

    Plasmid

    #114179

    Purpose

    Lentiviral vector for expression of tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ER

    Depositor

    Nicolas Demaurex

    Article

    Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

    Insert

    tagRFP-KDEL
    

    Use

    Lentiviral

    Tags

    KDEL retention signal and signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA-GalNAc-T2-GFP-ESCargo(FTV)

    Plasmid

    #140163

    Purpose

    Expresses a regulatable secretory cargo for mammalian cells and a Golgi marker

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    GalNAc-T2-msGFP2 and piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V) (GALNT2 Synthetic, Human)
    

    Use

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP-Sec22b-shR

    Plasmid

    #208358

    Purpose

    Mammalian expression of fluorescent N-terminally tagged shRNA resistant Sec22b

    Depositor

    Paula Nunes-Hasler

    Article

    Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

    Insert

    Sec22b (Sec22b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    four silent mutations introduced to render shRNA …

    Promoter

    CMV

    Available since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET19-MTdTc302

    Plasmid

    #207458

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    MTdTc302 (Dntt Mouse)
    

    Use

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    Cys188Ala, Cys216Ser, Cys378Ala, and Cys438Ser

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPRKCZ-C20-EGFP (C-PRKCZ-C20)

    Plasmid

    #217761

    Purpose

    Fluorescent reporter for ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Protein kinase C zeta (PRKCZ Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C20 domain (aa 405-592)

    Promoter

    CMV

    Available since

    June 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pNES-PRKCZ-C20-EGFP (C-NES-PRKCZ-C20)

    Plasmid

    #217763

    Purpose

    Fluorescent reporter for ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Protein kinase C zeta (PRKCZ Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    NES (ALQKKLEELELDEAPVAT) plus C20 domain (aa 405-…

    Promoter

    CMV

    Available since

    June 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pEGFP-PRKCZ-C20 (N-PRKCZ-C20)

    Plasmid

    #217762

    Purpose

    Fluorescent reporter for ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Protein kinase C zeta (PRKCZ Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Linker GGSSGGGA plus C20 domain (aa 405-592)

    Promoter

    CMV

    Available since

    June 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge