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Showing: 341 - 360 of 3076 results

  8. pET28a-mOct4 Full length(FL)

    Plasmid
    #206394
    Purpose
    Expresses Oct4 with a 6xHis tag for protein purification
    Depositor
    Ralf Jauch
    Insert
    Oct4 (Pou5f1 Mouse)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    206394-plasmid-map-sequence-id-409984

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  11. sgFFL_d23

    Plasmid
    #59892
    Purpose
    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with two seed sites mutated
    Depositor
    Georg Seelig
    Article
    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.
    Insert
    mCherry with intron containing the mouse mir-124–3
    Use
    Tags
    PEST and VAMP 3 UTR
    Expression
    Mammalian
    Mutation
    VAMP 3 UTR--3rd and fourth seed site removed
    Promoter
    doxycycline (Dox)-inducible promoter (CMV/TO)
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    59892-plasmid-map-sequence-id-108249

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  12. sgFFL_d3

    Plasmid
    #59891
    Purpose
    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with one seed site mutated.
    Depositor
    Georg Seelig
    Article
    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.
    Insert
    mCherry with intron containing the mouse mir-124–3
    Use
    Tags
    PEST and VAMP 3 UTR
    Expression
    Mammalian
    Mutation
    VAMP 3 UTR--3rd seed site removed
    Promoter
    doxycycline (Dox)-inducible promoter (CMV/TO)
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    59891-plasmid-map-sequence-id-108247

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  14. BtARR3 long

    Plasmid
    #236266
    Purpose
    Bacterial expression of bovine arrestin-3 long isoform
    Depositor
    Vsevolod Gurevich
    Insert
    arrestin-3 (ARR3 Bovine)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    long isoform
    Promoter
    lac
    Available since
    May 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    236266-plasmid-map-sequence-id-472329

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  15. pHS29

    Plasmid
    #171205
    Purpose
    bacterial expression for HIS purification of MEG-3 with mutations in the HMG-like motif
    Depositor
    Geraldine Seydoux
    Article
    Schmidt et al bioRxiv 2020.10.15.340570
    Insert
    meg-3 (meg-3 Nematode)
    Use
    Tags
    6xHIS
    Expression
    Bacterial
    Mutation
    F705A,Y708A,Y713A, N725A
    Promoter
    Available since
    June 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    171205-plasmid-map-sequence-id-335784

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  16. pAAP2

    Plasmid
    #171208
    Purpose
    bacterial expression for purification of PGL-3, allowing cleavage for MBP tag using TEV protease
    Depositor
    Geraldine Seydoux
    Article
    Schmidt et al bioRxiv 2020.10.15.340570
    Insert
    pgl-3 (pgl-3 Nematode)
    Use
    Tags
    MBP::HIS::TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    171208-plasmid-map-sequence-id-335799

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  17. pOpen-Endonuclease IV E. coli

    Plasmid
    #165551
    Purpose
    DNA AP endonuclease; Catalyzes the cleavage of DNA phosphodiester backbone at AP sites via hydrolysis leaving a 1 nucleotide gap with 3'-hydroxyl and 5' deoxyribose phosphate (dRP) termini; Also has 3'-diesterase activity which can remove 3' phosphate, 3'-alpha, beta-unsaturated aldehyde, phosphoglycoaldehyde, and other 3' blocking groups.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Endonuclease IV E. coli
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165551-plasmid-map-sequence-id-339868

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  18. sgFFL_3x_siRNA

    Plasmid
    #59893
    Purpose
    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with three siRNA-like sites
    Depositor
    Georg Seelig
    Article
    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.
    Insert
    mCherry with intron containing the mouse mir-124–3
    Use
    Tags
    PEST and VAMP 3 UTR
    Expression
    Mammalian
    Mutation
    VAMP 3 UTR--has three siRNA-like targets (fully c…
    Promoter
    doxycycline (Dox)-inducible promoter (CMV/TO)
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    59893-plasmid-map-sequence-id-108250

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Showing: 341 - 360 of 3076 results