We narrowed to 1,752 results for: krab

1. pBK2047-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA2)

   Plasmid

   #223167

   Purpose

   2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA2 targeting human/mouse APOE

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   KRAB-MECP2 (MECP2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310 (MECP2)

   Promoter

   EF1a

   Available since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV hU6-Non-target sgRNA Nestin-dCas9-KRAB-T2a-GFP

   Plasmid

   #196989

   Purpose

   Derived from pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP with non-target sgRNA

   Depositor

   Elizabeth Kirby

   Article

   Dause et al Mol Neurobiol. 2025 Mar 25. doi: 10.1007

   Insert

   Non-Target
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBK2046-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA1)

   Plasmid

   #223166

   Purpose

   2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting human/mouse APOE

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   KRAB-MECP2 (MECP2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310 (MECP2)

   Promoter

   EF1a

   Available since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCC_09 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9-NLS-2A-Puro-WPRE

   Plasmid

   #139094

   Purpose

   Expresses human codon-optimized inactive SpCas9 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   KRAB-dSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJT337 Lenti pEF-dCas9-3XFLAG-ZN471 KRAB-T2A-tagBFP-P2A-Blast

   Plasmid

   #187341

   Purpose

   dCas9 effector fusion for manipulating transcription

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   dCas9-3XFLAG-ZN471 KRAB-T2A-tagBFP-P2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1a

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCC_12 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9NG-NLS-2A-Puro-WPRE

   Plasmid

   #139097

   Purpose

   Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   KRAB-dxCas9-NG
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCC_10 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9NG-NLS-2A-Puro-WPRE

   Plasmid

   #139095

   Purpose

   Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   KRAB-dSpCas9-NG
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJT241 Lenti pEF-dCas9-3XFLAG-WT ZNF10 KRAB-T2A-tagBFP-P2A-Blast

   Plasmid

   #187326

   Purpose

   dCas9 effector fusion for manipulating transcription

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   dCas9-3XFLAG-WT ZNF10 KRAB-T2A-tagBFP-P2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1a

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCC_11 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9-NLS-2A-Puro-WPRE

   Plasmid

   #139096

   Purpose

   Expresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   KRAB-dxCas9 3.7
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A, A262T, R324L,S409I, E480K, E543D, M6…

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJT151 lenti pEF-rTetR(SE-G72P)-3XFLAG-ZNF10 KRAB-T2A-mCherry-BSD-WPRE

    Plasmid

    #188757

    Purpose

    Lentiviral backbone containing ZNF10 KRAB as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    ZNF10 KRAB (ZNF10 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSLQ2813 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-ABI-tagBFP-SpdCas9

    Plasmid

    #84242

    Purpose

    Expresses GA-inducible KRAB-Sp dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sp dCas9
    GID1-KRAB
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and PGK

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. PJT220 lenti pEF-rTetR(SE-G72P)-3XFLAG-ZNF473 KRAB-T2A-mCherry-BSD-WPRE

    Plasmid

    #188759

    Purpose

    Lentiviral backbone containing ZNF473 KRAB as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    ZNF473 KRAB (ZNF473 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLQ2818 pPB: CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

    Plasmid

    #84241

    Purpose

    Expresses ABA-inducible KRAB-Sp dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    ABI-tagBFP-Sp dCas9
    PYL1-KRAB
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and PGK

    Available since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLQ2818_Blasti_pPB: CAG-PYL1-KRAB-IRES-Blasti-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9-FKBP_F36V

    Plasmid

    #187959

    Purpose

    Expressed ABA-inducible dimerizing KRAB-dCas9 system, with KRAB-IRES-Blasticidin resistance under CAG promoter and tagBFP-dCas9-FKBP12 (F36V mutant) degron under PGK promoter in a piggyBac plasmid.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Inserts

    Blasticidin resistance
    FKBP12_F36V
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS linker

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSLQ2822 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI

    Plasmid

    #106895

    Purpose

    Expresses Sp dCas9 and GA-inducible KRAB for NOR gate/diametric regulation

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    GAI-tagBFP-Sp dCas9-ABI
    GID1-KRAB
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and PGK

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJT340 Lenti pEF-dCas9-3XFLAG-KRAB[WSR7EEE] + MGA1+2 + RYBP-T2A-tagBFP-P2A-Blast

    Plasmid

    #187344

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-KRAB[WSR7EEE] + MGA1+2 + RYBP-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    KRAB WSR7EEE

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJT242 Lenti pEF-dCas9-3XFLAG-ZNF10 KRAB[WSR7EEE] HighExpr-T2A-tagBFP-P2A-Blast

    Plasmid

    #187327

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-ZNF10 KRAB[WSR7EEE] HighExpr-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    KRAB WSR7EEE

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJT341 Lenti pEF-dCas9-3XFLAG-KRAB[WSR7EEE] + RYBP + MGA1+2-T2A-tagBFP-P2A-Blast

    Plasmid

    #195540

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti pEF-dCas9-3XFLAG-KRAB[WSR7EEE] + RYBP + MGA1+2-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LIR-wtloxP-TRE-CAG-Frt-red-puro-3xPA-Frt-GFP(mVenus)-KrasG12D-cMyc-SV40LT-IRES-KRABoff-PA-TRE-loxP257-RIR

    Plasmid

    #67277

    Purpose

    inducbile color change and cancer regulation: FlpO recombinase dependent expression of KrasG12D cMyc and SV40 large T antigen with doxycycline inducible downregulation through tetKRAB system.

    Depositor

    Jannik Elverløv-Jakobsen

    Article

    Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do

    Inserts

    first casette contain a red fluorescent protein (katushka) linked to puromycin resitance gene through an E2A site
    second cassette contains mVENUS-kras-cmyc-SV40LT-KRABoff
    

    Use

    Tags

    mTQ2 blue fluorescent and red flourescent gene ka…

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMP315

    Plasmid

    #134994

    Purpose

    KRAB-m6A Reader. Repress transcription near Gm6ATC sites. pUBC-KRAB-NLS-DpnI(aa146-254)

    Depositor

    Ahmad Khalil

    Article

    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.

    Insert

    pUBC-KRAB-NLS-DpnI(aa146-254)
    

    Use

    Tags

    DpnI(aa146-254) and KRAB domain

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge