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We narrowed to 12,560 results for: LIC

Showing: 8951 - 9000 of 12560 results

  14. PB-iRFP-STOP-ReNL

    Plasmid
    #113965
    Purpose
    Piggybac transposon plasmid CRISPR gene deletion activatable fluorescence. Constitutive iRFP670 under EF1A promoter, CMV promoter with two SV40 polyA followed by red-enhanced nanolantern (ReNL)
    Depositor
    Jordan Green
    Article
    Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:
    Inserts
    iRFP670
    ReNL
    Use
    Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV - SV40-PolyA - SV40-PolyA and EF1A
    Available since
    Dec. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    113965-plasmid-map-sequence-id-223037

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  19. pNIC-ZB-SPRTN-Y117C-FL

    Plasmid
    #110219
    Purpose
    Expression in E. coli of full-length SPRTN protein carrying Y112A mutation, a catalytically impaired mutant, with His and ZB tags at N-terminus.
    Depositor
    Kristijan Ramadan
    Article
    Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi
    Insert
    SPRTN (SPRTN Human)
    Use
    Tags
    His6 and ZB
    Expression
    Bacterial
    Mutation
    Y117C, P296L (see depositor comments below)
    Promoter
    Available since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    110219-plasmid-map-sequence-id-210947

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  38. BD-pGEX5X3-BD-SARS-CoV-2-S-tail-Thr1273Glu

    Plasmid
    #218458
    Purpose
    Bacterial expression plasmid for the SARS-CoV-2 cytosolic tail (Thr1273Glu substitution) with an N-terminal GST tag
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:
    Insert
    Cytosolic tail of SARS-CoV-2 spike carrying Thr1273Glu substitution and an N-terminal GST-tag (S SARS-CoV-2)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Thr1273Glu substitution
    Promoter
    Available since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    218458-plasmid-map-sequence-id-466904

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  39. BD-pGEX5X3-BD-SARS-CoV-2-S-tail-His1271Lys

    Plasmid
    #218456
    Purpose
    Bacterial expression plasmid for the SARS-CoV-2 cytosolic tail (His1271Lys substitution) with an N-terminal GST tag
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:
    Insert
    Cytosolic tail of SARS-CoV-2 spike carrying His1271Lys substitution and an N-terminal GST-tag (S SARS-CoV-2)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    His1271Lys substitution
    Promoter
    Available since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    218456-plasmid-map-sequence-id-466903

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  40. BD-pGEX5X3-SARS-CoV-2-S-tail-Lys1269Ala-His1271Ala

    Plasmid
    #218459
    Purpose
    Bacterial expression plasmid for the SARS-CoV-2 cytosolic tail (Lys1269Ala and His1271Ala substitutions) with an N-terminal GST tag
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:
    Insert
    Cytosolic tail of SARS-CoV-2 spike carrying Lys1269 and His1271 substitutions and an N-terminal GST-tag (S SARS-CoV-2)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Lys1269Ala and His1271Ala substitutions
    Promoter
    Available since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    218459-plasmid-map-sequence-id-466902

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  42. *MtbCM-HiBiT-His

    Plasmid
    #228028
    Purpose
    Expresses secreted Mycobacterium tuberculosis (M. tb) chorismate mutase (CM) containing a C-terminal HiBiT sequence and His tag
    Depositor
    James Inglese
    Article
    van Neer et al ACS Infect Dis. 2025 Mar 14;11(3):703-71
    Insert
    Rv1885c Chorismate Mutase (Rv1885c Mycobacterium tuberculosis H37Rv)
    Use
    Tags
    10xHis-tag with thrombin site and HiBiT Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promotor
    Available since
    March 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    228028-plasmid-map-sequence-id-451757

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  43. *MtbCM-bio-His

    Plasmid
    #186027
    Purpose
    Expresses secreted Mycobacterium tuberculosis (M. tb) chorismate mutase (CM) containing a C-terminal biotinylation site and His tag
    Depositor
    James Inglese
    Article
    van Neer et al ACS Infect Dis. 2025 Mar 14;11(3):703-71
    Insert
    Rv1885c Chorismate Mutase
    Use
    Tags
    Avi-tag for in vitro biotinylation and His-tag wi…
    Expression
    Bacterial
    Mutation
    Codon optimization for expression in BL21DE3
    Promoter
    T7 Promotor
    Available since
    March 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    186027-plasmid-map-sequence-id-370660

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  44. pCR4_miniFXN_7

    Plasmid
    #234360
    Purpose
    Mini FXN plasmid with a partial deletion of the 5' UTR and intron 1 in FXN. Does not contain GAA repeats.
    Depositor
    Marek Napierala
    Article
    Li et al Hum Gene Ther. 2020 Aug;31(15-16):839-85
    Insert
    FXN (FXN Human)
    Use
    Cloning
    Tags
    Flag Tag
    Expression
    Mutation
    There is a partial deletion in the 5'UTR of …
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    234360-plasmid-map-sequence-id-464761

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  45. Epac-S-H327

    Plasmid
    #228243
    Purpose
    mT2Del_EPACdDEPCD_Q270E_M312L_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187
    Insert
    mT2Del_EPACdDEPCD_Q270E_M312L_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228243-plasmid-map-sequence-id-451727

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  46. Epac-S-H328

    Plasmid
    #228244
    Purpose
    mT2Del_EPACdDEPCD_Q270E_M312L_E325T_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187
    Insert
    mT2Del_EPACdDEPCD_Q270E_M312L_E325T_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228244-plasmid-map-sequence-id-451838

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  47. Epac-S-H329

    Plasmid
    #228245
    Purpose
    mT2Del_EPACdDEPCD_Q270E_E325T_L777A_K778A_tdBlackcp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Zanetti et al FEBS Lett. 2024 Dec 31. doi: 10.1002/187
    Insert
    mT2Del_EPACdDEPCD_Q270E_E325T_L777A_K778A_tdBlackcp173Venus(ST) (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    228245-plasmid-map-sequence-id-451790

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  49. pCAG CAPN7 V18D,F98D-OSF

    Plasmid
    #193045
    Purpose
    Mammalian expression plasmid encoding full-length CAPN7 with V18D and F98D mutations that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)
    Depositor
    Wesley Sundquist
    Article
    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75
    Insert
    CAPN7 (CAPN7 Human)
    Use
    Tags
    OSF (One-Strep-FLAG)
    Expression
    Mammalian
    Mutation
    V18D, F98D
    Promoter
    Chicken Beta-actin
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    193045-plasmid-map-sequence-id-378994

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  50. pCA528 IST1 L328D (316-366)(C-Cys)

    Plasmid
    #193035
    Purpose
    Bacterial expression plasmid for IST1 (316-366) with C-terminal Cysteine for fluorescence polarization binding assays. Contains L328D mutation that diminishes binding to MIT domains.
    Depositor
    Wesley Sundquist
    Article
    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75
    Insert
    IST1 (IST1 Human)
    Use
    Tags
    6xHis-Sumo
    Expression
    Bacterial
    Mutation
    L328D, Residues 316-366, Non-native C-terminal Cy…
    Promoter
    T7
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    193035-plasmid-map-sequence-id-379007

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Showing: 8951 - 9000 of 12560 results