We narrowed to 18,451 results for: Met

1. ST-SBP-EGFP_puromycin

   Plasmid

   #65270

   Purpose

   ST reporter only ( no hook) (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   truncated sialyltransferase (ST6GAL1 Human)
   

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 43 of ST

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEB2-mRFP1

   Plasmid

   #104001

   Purpose

   Plasmid encoding mRFP1

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mRFP1
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   Mutations relative to DsRed (R2A, K5E, N6D, T21S,…

   Promoter

   proC

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. spa-GCaMP6s

   Plasmid

   #67556

   Purpose

   photoactivatable fluorescent calcium reporter

   Depositor

   Ehud Isacoff

   Article

   Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

   Insert

   spa-GCaMP6s
   

   Tags

   HIS-tag

   Expression

   Mammalian

   Mutation

   K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

   Available Since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pLentiCRISPR-v1-sgMPC2_7

   Plasmid

   #163457

   Purpose

   lentiviral vector expressing Cas9 and an sgRNA targeting MPC2

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Insert

   sgRNA 7 targeting GPT2 (MPC2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiCRISPR-v1-sgMPC2_9

   Plasmid

   #163458

   Purpose

   lentiviral vector expressing Cas9 and an sgRNA targeting MPC2

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Insert

   sgRNA 9 targeting GPT2 (MPC2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-P1-N-miRFP670-C

   Plasmid

   #159438

   Purpose

   CRISPR knock-in donor construct with fluorescent reporter

   Depositor

   Jianhua Xing

   Article

   Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

   Insert

   miRFP670
   

   Use

   CRISPR

   Tags

   3' linker pair 1, 5' linker pair 1, C t…

   Promoter

   CMV

   Available Since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU1/Sm/3' Box_(GLuc)_INT

   Plasmid

   #68438

   Purpose

   Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct_bearing three PP7 Stem-loops
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U1

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-CB1R-V2tail-TevN-BLITz1-TetR-VP16

   Plasmid

   #89872

   Purpose

   expresses CB1R-iTango component in mammalian cells

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   CB1R-V2tail-TevN-BLITz1-TetR-VP16
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAVsc CB6PI Gpluc7XmiR-122T

   Plasmid

   #35647

   Depositor

   Phillip Zamore

   Article

   Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

   Insert

   7 bulged miR-122 target sites
   

   Use

   AAV

   Promoter

   CB

   Available Since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21-pelB-LaG-27

    Plasmid

    #172752

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-27
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFU-cMVIIA-PE

    Plasmid

    #24147

    Depositor

    Ines Ibanez-Tallon

    Article

    Auer et al Nat Methods. 2010 Mar;7(3):229-36. doi:

    Insert

    loxP-DsRed-loxP-omega-Conotoxin MVIIA-FLAG-PDGF_R_TM-EGFP (PDGFRB Human, Conus magus, Aequorea victoria)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR1A-3xFlag-M694V-MEFV

    Plasmid

    #167118

    Purpose

    Gateway entry plasmid with 3xFlag-pyrin (M694V/FMF variant)

    Depositor

    Thomas Henry

    Article

    Chirita et al Methods Mol Biol. 2022;2523:179-195. doi

    Insert

    MEFV (MEFV Human)
    

    Use

    Gateway entry vector

    Tags

    3xFlag

    Mutation

    M694V Methionine 694 to valine (FMF mutation)

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Str-KDEL_ManII-SBP-tagBFP

    Plasmid

    #65254

    Purpose

    synchronize trafficking of ManII from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin-KDEL and truncated Mannosidase II
    

    Tags

    Streptavidin Binding Protein (SBP) and tagBFP

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available Since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLpC-Reg-L-IFP-F[2]+Venus (mammalian)

    Plasmid

    #52903

    Purpose

    Binary retroviral pl. (puromycin). Reg:Linker (GGGGS)X2+IFP-F[2] (PacI/EcoRI). In the second MCS, the Venus fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    Reg-L-IFP-F[2]+Venus
    

    Use

    Retroviral

    Tags

    L-IFP-F[2]

    Expression

    Mammalian

    Promoter

    Unknown

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pLpC-Cat-L-IFP-F[1]+Plum (mammalian)

    Plasmid

    #52905

    Purpose

    Binary retroviral pl. (puromycin). Cat(PacI/ClaI):Linker (GGGGS)X2+IFP-F[2]. In the second MCS, the Plum fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    Cat-L-IFP-F[1]+Plum
    

    Use

    Retroviral

    Tags

    L-IFP-F[1]

    Expression

    Mammalian

    Promoter

    Unknown

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-P1-N-EGFP-C

    Plasmid

    #159436

    Purpose

    CRISPR knock-in donor construct with fluorescent reporter

    Depositor

    Jianhua Xing

    Article

    Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    3' linker pair 1, 5' linker pair 1, C t…

    Promoter

    CMV

    Available Since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Clover-Nup50-N-10

    Plasmid

    #56303

    Purpose

    Localization: Nuclear Pore, Excitation: 505, Emission: 515

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Nup50 (NUP50 Human)
    

    Tags

    Clover

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 18, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pUAST mito roGFP2-Orp1

    Plasmid

    #64997

    Purpose

    for expression of mitochondrial roGFP2-Orp1 with the Gal4 expression system in drosophila

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Tags

    roGFP2

    Expression

    Insect

    Mutation

    mitochondrial targeting sequence

    Available Since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBEAST-J23101-CocE

    Plasmid

    #114600

    Purpose

    "Strong constitutive expression of metabolic enzyme, CocE, for cell-free expression"

    Depositor

    Jerome Bonnet

    Article

    Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

    Insert

    CocE
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Promoter

    J23101

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV TBG FFluc miR122sponge

    Plasmid

    #35657

    Depositor

    Phillip Zamore

    Article

    Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

    Insert

    miR-122 sponge
    

    Use

    AAV

    Promoter

    TBG

    Available Since

    July 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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