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pSW_1

Plasmid

#71279

Purpose

Gateway destination vector

Depositor

Alexander Stark

Publication

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Type

Empty backbone

Use

Gateway vector

Tags

GAL4 DBD

Promoter

CMV

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pSGE_240_pAGW_V5

Plasmid

#71188

Purpose

Gateway destination vector

Depositor

Alexander Stark

Publication

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Type

Empty backbone

Use

Gateway Cloning

Tags

Gal4 DBD and V5

Promoter

actin 5C

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pId1-IRES-Venus

Plasmid

#22663

Depositor

Robert Benezra

Publication

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Inhibitor of DNA binding 1 (Id1 Mouse)

Tags

IRES-Venus

Expression

Mammalian

Available since

Jan. 20, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pId1-IRES-YFP

Plasmid

#22667

Depositor

Robert Benezra

Publication

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Inhibitor of DNA binding 1 (Id1 Mouse)

Tags

IRES-YFP

Expression

Mammalian

Available since

Jan. 20, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3 VP4-VP2

Plasmid

#216787

Purpose

Mammalian expression of the VP4-VP2 portion of the CVB3 Kandolf strain polyprotein

Depositor

Kevin Janes

Publication

Griffiths et al Sci Adv. 2024 Nov 15;10(46):eadp6303. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

VP4-VP2

Expression

Mammalian

Promoter

CMV

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pEN_TT CVB3 A67G

Plasmid

#216788

Purpose

Gateway entry vector for the Kandolf strain of CVB3 with a VP4 A67G mutation

Depositor

Kevin Janes

Publication

Griffiths et al Sci Adv. 2024 Nov 15;10(46):eadp6303. do

Insert

CVB3

Use

Gateway Cloning

Mutation

A67G in VP4

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer-YAP1

Plasmid

#213583

Purpose

Doxycycline inducible expression of YAP1 wild type cDNA in mammalian cells

Depositor

Channing Der

Publication

Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3_cAMPFIRE-L-R279E

Plasmid

#182282

Purpose

Mammalian expression of cAMPFIRE-L with R279E (non-cAMP-binding) mutation under a CMV promotor.

Depositor

Haining Zhong

Publication

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)

Expression

Mammalian

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_HA-BirA-ER

Plasmid

#113897

Purpose

Mammalian (inducible) expression of HA-tagged ER-resident E. coli biotin ligase

Depositor

A. Radu Aricescu

Publication

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Insert

E. coli biotin ligase

Use

Lentiviral

Tags

HA

Promoter

CMV-MIE-TetO2

Available since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pYPQ133A2.0

Plasmid

#99891

Purpose

Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

Depositor

Yiping Qi

Publication

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU6

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pECE HA AMPKa1 AS

Plasmid

#69831

Purpose

Expresses Expresses AMPK alpha 1 in mammalian cells

Depositor

Anne Brunet

Publication

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Tags

HA

Expression

Mammalian

Promoter

SV40 early promoter

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FUW-ubiquitin-SV40-RFP

Plasmid

#65448

Purpose

Expression of RFP in mammalian cells. Control vector for FUW-Ephrin-RFP vectors

Depositor

Eduard Batlle

Publication

Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

Insert

SV40-RFP

Use

Lentiviral

Expression

Mammalian

Promoter

UBQ

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pTol2-CreLite

Plasmid

#131783

Purpose

Tol2 destination vector carrying Ubi:CreLite with mTagBFP2 as a visual marker.

Depositor

George Eisenhoffer

Publication

Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PhyBCreC, PIF6CreN, and mTagBFP2

Use

Cre/Lox and Synthetic Biology; Zebrafish tol2 tra…

Tags

mTagBFP2

Expression

Mammalian

Promoter

Ubi

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR1a-mCherry-STIM1

Plasmid

#114176

Purpose

Gateway entry clone containing mCherry-STIM1

Depositor

Nicolas Demaurex

Publication

Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

Insert

STIM1 (STIM1 Human)

Use

Gateway Cloning

Tags

mCherry (inserted after signal peptide)

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pX459-HypaCas9-ECT2_sgRNA

Plasmid

#183873

Purpose

pX459V2.0-HypaCas9 plasmid with ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.

Depositor

Alisa Piekny

Publication

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ECT2 sgRNA spacer (ECT2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pODN-AAVS1-mR2-CAAX

Plasmid

#183871

Purpose

Repair template for the stable expression of a mRuby2-CAAX fusion in human cells using CRISPR/Cas9.

Depositor

Alisa Piekny

Publication

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AAVS1 homology arms with CMV-driven mRuby2-CAAX fusion (AAVS1 Human)

Use

CRISPR; Donor template

Tags

mRuby2-CAAX

Expression

Mammalian

Mutation

Homology arms contain point mutations to remove t…

Promoter

CMV

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pODN-mNG-ECT2

Plasmid

#183835

Purpose

Repair template for the N-terminal tagging of Ect2 with mNeonGreen in human cells using CRISPR/Cas9.

Depositor

Alisa Piekny

Publication

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ECT2 homology arms with mNeonGreen-linker (ECT2 Human)

Use

CRISPR; Donor template

Tags

mNeonGreen-linker

Expression

Mammalian

Mutation

Homology arms contain point mutations to remove t…

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA

Plasmid

#162802

Purpose

STELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient expression

Depositor

Simon Elsaesser

Publication

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-BsmBI-HA

Use

Piggybac

Tags

HA

Expression

Mammalian

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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p alpha-actinin:GAL4/UAS:TdTomato

Plasmid

#161712

Purpose

Express TdTomato through amplification of GAL4/UAS system

Depositor

Hollis Cline

Publication

Hiramoto et al Proc Natl Acad Sci U S A. 2014 Nov 25;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TdTomato, GAL4

Use

Xenopus laevis expression

Tags

TdTomato

Expression

Mammalian

Promoter

actin-GAL4, UAS

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCSIIbleo-PIF3-mEGFP

Plasmid

#139483

Purpose

Expresses PIF3-mEGFP in mammalian cells.

Depositor

Kazuhiro Aoki

Publication

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

PIF3 (PIF3 Mustard Weed)

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Mutation

PIF3 1-100 aa

Promoter

Human EF1a promoter

Available since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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