We narrowed to 13,723 results for: cas9

1. pX330A-1x4

   Plasmid

   #58768

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. M-NMcas

   Plasmid

   #48670

   Purpose

   Mammalian N. meningitidis Cas9 expression, human optimized

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Jan. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pDB4280

   Plasmid

   #98699

   Purpose

   A Cas9-encoding plasmid containing the 5' portion of the ura4 marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-ura4 system

   Depositor

   Li-Lin Du

   Article

   Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDB4281

   Plasmid

   #98700

   Purpose

   A Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX system

   Depositor

   Li-Lin Du

   Article

   Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX330A-1x5

   Plasmid

   #58769

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pX330A-1x6

   Plasmid

   #58770

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. DS-NMcas

   Plasmid

   #48646

   Purpose

   Bacterial N. meningitidis Cas9 (NM) + tracrRNA expression, cloDF13/spectinomycin

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Inserts

   Cas9
   NM tracrRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   NM tracrRNA promoter and proC

   Available since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX330A-1x7

   Plasmid

   #58771

   Purpose

   Expresses Cas9 nuclease and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 nuclease
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMLS485

   Plasmid

   #188890

   Purpose

   Worm Cas9 expression vector, Peft-3 promoter

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

   Insert

   Peft-3::Cas9
   

   Use

   Tags

   Expression

   Worm

   Mutation

   C. elegans codom optimization and intron addition

   Promoter

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAH237

    Plasmid

    #121146

    Purpose

    Expression of both nmt41p-cas9 and rrk1p-gRNA (LEU2 marker) for CRISPR genome editing in fission yeast

    Depositor

    Katsunori Tanaka

    Article

    Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

    Inserts

    gRNA cassette
    humanized Streptococcus pyogenes Cas9
    

    Use

    CRISPR

    Tags

    Flag

    Expression

    Yeast

    Mutation

    Promoter

    S.pombe rrk1 and nmt41

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMB66

    Plasmid

    #47946

    Purpose

    Expresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 promoter

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP

    Expression

    Worm

    Mutation

    Promoter

    hsp-16.48

    Available since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-dSa-VP64

    Plasmid

    #99680

    Purpose

    Expresses dSa Cas9 fused to gold standard VP64 activator

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VP64

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Oct. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. M-SPcas

    Plasmid

    #48668

    Purpose

    Mammalian S. pyogenes Cas9 expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. DS-ST1casN-

    Plasmid

    #48659

    Purpose

    Bacterial nuclease-null ST1 Cas9 expression

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Inserts

    Cas9, nuclease-null
    ST1 tracrRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D to A, DH to AA, N to A

    Promoter

    proC

    Available since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. DS-ST1cas

    Plasmid

    #48647

    Purpose

    Bacterial S. thermophilus #1 Cas9 (ST1) + tracrRNA expression, cloDF13/spectinomycin

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Inserts

    Cas9
    ST1 tracrRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    proC

    Available since

    Nov. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMB67

    Plasmid

    #47947

    Purpose

    Expresses C. elegans optimized Cas9 from the hsp-16.48 promoter

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and egl-13 NLS

    Expression

    Worm

    Mutation

    Promoter

    hsp-16.48

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. DS-TDcasN-

    Plasmid

    #48660

    Purpose

    Bacterial nuclease-null TD Cas9 expression

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Inserts

    Cas9, nuclease-null
    TD tracrRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D to A, DH to AA, N to A

    Promoter

    proC and tracrRNA promoter

    Available since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSHS293

    Plasmid

    #87348

    Purpose

    D435C/E945C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, lobe closure FRET construct

    Depositor

    Jennifer Doudna

    Article

    Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

    Insert

    Cas9
    

    Use

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    C80S/D435C/C574S/E945C

    Promoter

    T7

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. BCJJ344

    Plasmid

    #117503

    Purpose

    UBI10:Cas9:E9 Golden Gate Level 1 Position 2 reverse

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:tagt:UBI10:Cas9-3(intron):E9:ttgc:BpiI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtUBI10

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSHS248

    Plasmid

    #87354

    Purpose

    S867C/N1054C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-2 FRET construct

    Depositor

    Jennifer Doudna

    Article

    Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

    Insert

    Cas9
    

    Use

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    C80S/C574S/S867C/N1054C

    Promoter

    T7

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge