We narrowed to 2,462 results for: LARGE

1. pHH0103_LRP5_Ldl-recept-b

   Plasmid

   #109785

   Purpose

   Protein expression and purification of LRP5_Ldl-recept-b

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   LRP5_Ldl-recept-b (LRP5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDM304_YFP-IqgC_mRFP-Raf1(RBD)

   Plasmid

   #128698

   Purpose

   simultaneous expression of YFP-tagged IqgC and mRFP-tagged active Ras probe (GTPase binding domain from Raf1 kinase) in Dictyostelium discoideum cells

   Depositor

   Igor Weber

   Article

   Marinovic et al Proc Natl Acad Sci U S A. 2019 Jan 22;11

   Inserts

   iqgC (full length)
   Raf1 kinase (aa 50-132)
   

   Use

   Dictyostelium discoideum expression vector

   Tags

   YFP and mRFP

   Expression

   Mutation

   none

   Promoter

   Available since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOpen-K12polLF (exo-)

   Plasmid

   #165522

   Purpose

   DNA pol fragment used in flourescent labelling for microarray, dA and dT tailing, and ligating DNA adapters to DNA fragments.

   Depositor

   Jennifer Molloy, Drew Endy, FreeGenes Project

   Insert

   DNA Polymerase I, Large (Klenow) Fragment (3'-5' exo-)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pET28-H12TEV

   Plasmid

   #208635

   Purpose

   for E. coli expression of large protein, insert 6xhis + TEV site using NdeI and BamHI site in pET28a(+), note that it is 6xHis-Thrombin-6xHis-TEV, not continuous 12xHis. BamHI in-frame with tag.

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Use

   Tags

   6xhis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOpen-BstpolLF

   Plasmid

   #165553

   Purpose

   Fragment retains 5'-3' polymerase activity from full length Bst DNA Polymerase, while lacking 5'-3' exonuclease activity. Suitable for applications requiring thermophilic strand displacement.

   Depositor

   Jennifer Molloy, Drew Endy, FreeGenes Project

   Insert

   Bst DNA Polymerase, Large Fragment
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 16, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. Sc FLAG-Pol2-pRS425/GAL

   Plasmid

   #241978

   Purpose

   Over-express Sc FLAG-Pol2 (Pol epsilon large subunit) in yeast (episomal)

   Depositor

   Michael O'Donnell

   Insert

   FLAG-Pol2 (POL2 Budding Yeast)
   

   Use

   Tags

   3X FLAG

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Sc Pol1-FLAG-pRS402/GAL-L

   Plasmid

   #241975

   Purpose

   Over-express Sc Pol1-FLAG (Sc pol alpha - large subunit) in yeast (integrated)

   Depositor

   Michael O'Donnell

   Insert

   Pol1-FLAG (POL1 Budding Yeast)
   

   Use

   Tags

   3X FLAG

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. ALDH18A1

   Plasmid

   #155402

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   ALDH18A1 (ALDH18A1 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. HSPB1

   Plasmid

   #155670

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   HSPB1 (HSPB1 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21a(+)-Bst LF-6xHis

    Plasmid

    #159148

    Purpose

    expresses His-tagged large fragment of Bst polymerase

    Depositor

    Andrea Pauli

    Article

    Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d

    Insert

    large fragment of Geobacillus stearothermophilus DNA polymerase I
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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