We narrowed to 6,274 results for: nls
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Plasmid#197047PurposepCYC-NLS-MCP-2xGFP on pRS305DepositorInsertMCP-2xGFP
ExpressionYeastPromoterCYC1Available SinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTPGI_dCas9
Plasmid#64279Purposeencodes yeast-optimized dCas9 synthetic transcription factorDepositorInsertYeast-optimized dCas9
TagsSV40 NLSExpressionYeastMutationD10A & H840A relative to S. pyogenes Cas9PromoterpTPGIAvailable SinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huMb3Cas12a
Plasmid#174677PurposeExpresses 6xHis-MBP tagged Mb3Cas12aDepositorArticleInserthuMb3Cas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV and NLS, 6xHis, 3xHAExpressionBacterialPromoterT7Available SinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS2-YFP
Plasmid#27122DepositorInsertMS2
TagsEYFP, HA, and SV40 NLSExpressionMammalianAvailable SinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Cry2FL-VP64
Plasmid#60554PurposeExpresses Cry2FL-VP64 in mammalian cellsDepositorInsertCry2FL-VP64
TagsFLAG, HA tag, and SV40 NLSExpressionMammalianPromoterCMVAvailable SinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS42H_PTEF1_dCas9_TCYC1
Plasmid#177155PurposeExpresses dCas9 in yeast cellsDepositorInsertNuclease-null Cas9
TagsSV40 NLSExpressionYeastPromoterTEF1 promoterAvailable SinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
vHB16
Plasmid#193455PurposeExpression of GFP-Cas9-NLS and gRNA in A. castellaniiDepositorInserttRNA-gRNA
UseCRISPRPromoterU6Available SinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLQ11438
Plasmid#183204PurposepHR-EF1a-ABE8e-hyperdLbCas12a-NLS-mCherry-P2A-Puro-WPREDepositorInserthyperdCas12 base editor
UseCRISPR and LentiviralAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKIR1.0
Plasmid#86409PurposeCRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.DepositorInserthuman-codon-optimized SpCas9
UseCRISPRTagsFLAG and NLSPromoterAtRPS5AAvailable SinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC2HB
Plasmid#63734Purposebait plasmid for an adapted two-hybrid system for C. albicansDepositorTypeEmpty backboneUseC. albicans bait plasmidTagsHA, LexA, and NLSPromoterMet3Available SinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC2HP
Plasmid#63735Purposeprey plasmid for an adapted two-hybrid system for C. albicansDepositorTypeEmpty backboneUseC. albicans prey plasmidTagsFLAG, NLS, and VP domainPromoterMet3Available SinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
EZ-L560
Plasmid#173028PurposeEncodes OptoAMP2 circuitDepositorInsertVP16-EL222
TagsSV40 NLSExpressionYeastAvailable SinceSept. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLSLGFP
Plasmid#51494PurposepLSL with GFP followed by the us:NPTII repair template, Rep is not included on this plasmidDepositorInsertsGFP
us:NPTII repair template
UsePlant t-dna plasmidTagsNLS and flagPromotervirion-sense LIR and 2x35SAvailable SinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9-VRER-P2A-mScarlet (MNW003)
Plasmid#174138PurposeCMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarletDepositorInserthuman codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-mScarlet
UseIn vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-mScarletExpressionMammalianMutationVRER=D1135V/G1218R/R1335E/T1337RPromoterCMV and T7Available SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
EZ-L175
Plasmid#173025PurposeEncodes OptoAMP1 circuitDepositorInsertVP16-EL222
TagsSV40 NLSExpressionYeastAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
MS2-Snapf
Plasmid#65860PurposeExpresses RNA-binding protein MS2 with fast SNAP fusion tagDepositorInsertsMS2
Fast SNAP
TagsHA and NLSExpressionBacterial and YeastAvailable SinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLEXY (pDN137)
Plasmid#72656Purposegolden gate entry vector for mCherry-LEXY tagging of proteins (no constitutive NLS)DepositorTypeEmpty backboneUseGolden gate entry vectorTagsmCherry-LEXYExpressionMammalianPromoterCMV-FAvailable SinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKF295
Plasmid#72871Purposeexpression of improved FLP recombinaseDepositorAvailable SinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huBoCas12a
Plasmid#174673PurposeExpresses 6xHis-MBP tagged BoCas12aDepositorArticleInserthuBoCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV and NLS, 6xHis, 3xHAExpressionBacterialPromoterT7Available SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLQ11440
Plasmid#183203PurposepHR-TRE3G-ABE8e-hyperdLbCas12a-NLS-P2A-mCherry-EFS-Puro-WPREDepositorInserthyperdCas12 base editor
UseCRISPR and LentiviralAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only