We narrowed to 6,963 results for: RAP

1. AA023

   Plasmid

   #215931

   Purpose

   Fragmid fragment: (guide cassette) guide expression; positive control cell surface

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; sgCD81_v1 [Sp]; trRNA_v2 [Sp]; CS2
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AA102

   Plasmid

   #215935

   Purpose

   Fragmid fragment: (guide cassette) guide expression; reverse orientation; enables iBar UMIs

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   rev{U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v5}
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AA107

   Plasmid

   #215936

   Purpose

   Fragmid fragment: (guide cassette) guide expression; enables iBar UMIs

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v5
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMV_AA091

   Plasmid

   #216106

   Purpose

   CRISPRa, gRNA expression with modified trRNA to recruit activators via PP7 tags (guide only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   gRNA expression with modified trRNA to recruit activators via PP7 tags
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AAV2_CAG_oROS-G_LF(C199S)

   Plasmid

   #216116

   Purpose

   Encodes the loss-of-function mutation C199S of the genetically encoded, green fluorescent peroxide sensor oROS-G in AAV viral vectors.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.01.31.578117

   Insert

   oROS-G_LF(C199S)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. YIp128-CUP1-His6-CUbo(K48R)-GFP

   Plasmid

   #212793

   Purpose

   Copper-inducible expression of the artificial test substrate His6-CUbo(K48R)-GFP in budding yeast

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   His6-CUbo(K48R)-GFP
   

   Use

   Integrative vector

   Tags

   Expression

   Yeast

   Mutation

   CUb(K48R)

   Promoter

   CUP1

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. YIp128-CUP1-His6-CUbo(K63R)-GFP

   Plasmid

   #212794

   Purpose

   Copper-inducible expression of the artificial test substrate His6-CUbo(K63R)-GFP in budding yeast

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   His6-CUbo(K63R)-GFP
   

   Use

   Integrative vector

   Tags

   Expression

   Yeast

   Mutation

   CUb(K63R)

   Promoter

   CUP1

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. YIp211-ADH-NUbo-E3(48) aa24-150-VSV

   Plasmid

   #212747

   Purpose

   Constitutive expression of NUbo-E3(48)ΔU7BD-VSV in budding yeast, inactive E3(48)

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   E3(48) aa24-150
   

   Use

   Integrative vector

   Tags

   NUbo and VSV

   Expression

   Yeast

   Mutation

   Cue1 ΔU7BD

   Promoter

   ADH1

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. YIp211-ADH-myc-E3(1) C885A-CUbo-VSV

   Plasmid

   #212738

   Purpose

   Consitutive expression of myc-E3(1) C885A-CUbo-VSV in budding yeast, catalytic inactive E3(1)

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   E3(1)
   

   Use

   Integrative vector

   Tags

   CUbo-NLS-VSV and myc

   Expression

   Yeast

   Mutation

   HOIP(C885A)

   Promoter

   ADH1

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7

    Plasmid

    #212805

    Purpose

    Constitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7 in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMX1463

    Plasmid

    #208805

    Purpose

    Expresses plasma-membrane-bound FRB-SBFP2 for rapid relocalization of cytoplasmic proteins tagged with FKBP upon addition of rapamycin

    Depositor

    Linda Wordeman

    Article

    Wordeman et al Mol Biol Cell. 2016 Apr 15;27(8):1300-9.

    Insert

    FRB (CRP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SVA-lncRNA AK057321 shRNA 1S scramble

    Plasmid

    #202837

    Purpose

    Lentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 1S

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    SVA-lncRNA AK057321 shRNA 1S scramble control
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    6

    Available since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SVA-lncRNA AK057321 shRNA 2S scramble

    Plasmid

    #202839

    Purpose

    Lentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 2S

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    SVA-lncRNA AK057321 shRNA 2S scramble control
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGL3-luciferase-reporter-JAM2-SVA

    Plasmid

    #202832

    Purpose

    pGL3 luciferase reporter with JAM2 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    JAM2 intronic SVA
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL3-luciferase-reporter-POFUT2-SVA

    Plasmid

    #202831

    Purpose

    pGL3 luciferase reporter with POFUT2 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    POFUT2 intronic SVA
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGL3-luciferase-reporter-HTT-SVA

    Plasmid

    #202833

    Purpose

    pGL3 luciferase reporter with HTT SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    HTT intronic SVA
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGL3-luciferase-reporter-CHAF1B-SVA

    Plasmid

    #202829

    Purpose

    pGL3 luciferase reporter with CHAF1B SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    CHAF1B intronic SVA
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SVA-lncRNA AK057321 shRNA 1A scramble control

    Plasmid

    #202835

    Purpose

    Lentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 1A

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    pLVX-SVA-lncRNA shRNA 1A scramble control
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCENPBR pc861

    Plasmid

    #167569

    Purpose

    Human CENP-B (aa 1-169 containing DNA-binding domain) cloned into pDsRed1-N1 vector (Clontech).

    Depositor

    Cristina Cardoso

    Article

    Chagin et al Nucleus. 2019 Dec;10(1):231-253. doi: 10

    Insert

    Homo sapiens centromere protein B (CENPB Human)
    

    Use

    Tags

    Ds-Red

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTol2zmiRFP-EF1-2xCMV-EF1-mClover3

    Plasmid

    #196112

    Purpose

    mammalian/zebrafish expression of zmiRFP-EF1-2xCMV-EF1-mClover3

    Depositor

    Reinhard Köster

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    zmiRFP-EF1-2xCMV-EF1-mClover3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTol2zmiRFP2-EF1-2xCMV-EF1-mClover3

    Plasmid

    #196111

    Purpose

    mammalian/zebrafish expression of zmiRFP2-EF1-2xCMV-EF1-mClover3

    Depositor

    Reinhard Köster

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    zmiRFP2-EF1-2xCMV-EF1-mClover3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMeCP2miRFP670 pc3942

    Plasmid

    #167568

    Purpose

    The miRFP670-gene is ligated c-terminally to the rat meCP2-gene.

    Depositor

    Cristina Cardoso

    Article

    Chagin et al Nucleus. 2019 Dec;10(1):231-253. doi: 10

    Insert

    MeCp2 (Mecp2 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTol2zTagRFP658-EF1-2xCMV-EF1-mClover3

    Plasmid

    #196109

    Purpose

    mammalian/zebrafish expression of zTagRFP658-EF1-2xCMV-EF1-mClover3

    Depositor

    Reinhard Köster

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    zTagRFP658-EF1-2xCMV-EF1-mClover3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTol2zemiRFP2-EF1-2xCMV-EF1-mClover3

    Plasmid

    #196110

    Purpose

    mammalian/zebrafish expression of zemiRFP2-EF1-2xCMV-EF1-mClover3

    Depositor

    Reinhard Köster

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    zemiRFP2-EF1-2xCMV-EF1-mClover3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pXPR_003 sgINTS12 guide 2

    Plasmid

    #193607

    Purpose

    INTS12 knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgINTS12 guide 2 (INTS12 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pXPR_003 sgINTS12 guide 1

    Plasmid

    #193606

    Purpose

    INTS12 knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgINTS12 guide 1 (INTS12 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET-Duet1-6xHis-TEV-OPTN

    Plasmid

    #190192

    Purpose

    Plasmid for the expression of 6xHis-TEV-OPTN. Internal reference: SMC397

    Depositor

    Sascha Martens

    Article

    Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    optineurin (OPTN Human)
    

    Use

    Tags

    6xHis and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKeratin-emiRFP2-N1

    Plasmid

    #178972

    Purpose

    Expresses the fusion protein of Keratin-emiRFP2 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    Keratin-emiRFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV-miRFP2-IRES-HO1-N1

    Plasmid

    #178967

    Purpose

    Expresses miRFP2 and HO1 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    miRFP2
    

    Use

    Tags

    HO1 and no

    Expression

    Mammalian

    Mutation

    NO

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLPC-MYC.Mm.EPC1

    Plasmid

    #184653

    Purpose

    Retroviral expression of mouse Epc1

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    Epc1 (Epc1 Mouse)
    

    Use

    Retroviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge