We narrowed to 12,112 results for: LEA

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pMSSM151: pU6-NlovFz2_omegaRNA-entry

Plasmid

#205263

Purpose

Human expression vector for NlovFz2 omegaRNA (BsmBI guide entry site)

Depositor

Feng Zhang

Publication

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

NlovFz2 omegaRNA

Expression

Mammalian

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ333: pU6-MmeFz2_omegaRNA-entry

Plasmid

#205267

Purpose

Human expression vector for MmeFz2 omegaRNA (BbsI guide entry site)

Depositor

Feng Zhang

Publication

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

MmeFz2 omegaRNA

Expression

Mammalian

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pUbi-MAD7

Plasmid

#170133

Purpose

For plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Caixia Gao

Publication

Lin et al J Genet Genomics. 2021 May 3. pii: S1673

Insert

MAD7(ErCas12a)

Use

CRISPR

Expression

Plant

Promoter

maize Ubiquitin-1

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a - SodA

Plasmid

#89612

Purpose

Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase A protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Publication

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Superoxide dismutase A

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2+ MBIC5-mCer3

Plasmid

#74116

Purpose

For generating mRNA for zebrafish injection of cytoplasmic fluorogen-activating protein (FAP)

Depositor

Michael Tsang

Publication

He et al Nat Methods. 2016 Mar;13(3):263-268. doi

Insert

MBIC5-mCer3

Use

Transgenic construct

Tags

mCerulean 3

Promoter

Myl7 (Cmlc2)

Available since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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mitoZFD_ND1-Left-G1397-N

Plasmid

#180768

Purpose

Expresses mitoZFD_ND1-Left-G1397-N(DddAtox half) in mammalian cells

Depositor

Jin-Soo Kim

Publication

Lim et al Nat Commun. 2022 Jan 18;13(1):366. doi:

Insert

mitoZFD for ND1 mutation

Expression

Mammalian

Promoter

pCMV

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-LIC

Plasmid

#120247

Purpose

For LIC cloning with N-terminal V5 tag

Depositor

Larry Gerace

Publication

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

V5

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJY3919

Plasmid

#113332

Purpose

Hermes transposable plasmid; TIR-flanked LEU2, recoded transposase driven by TEF1 promoter

Depositor

Suzanne Sandmeyer

Publication

Patterson et al Metab Eng. 2018 May 22;48:184-196. doi:

Inserts

beta-isopropyl malate dehydrogenase
codon-optimized Hermes transposase

Expression

Yeast

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p3-K230-FokI_L (DAS)

Plasmid

#32546

Depositor

Jin-Soo Kim

Publication

Lee et al Genome Res. 2010 Jan;20(1):81-9. Epub 20

Insert

ZFN

Tags

HA epitope

Expression

Mammalian

Promoter

cmv

Available since

Dec. 2, 2011

Availability

Academic Institutions and Nonprofits only
pAFi-dHdrA

Plasmid

#184907

Purpose

carrying CRISPR/dCas9 system for gene knockdown in Acidithiobacillus ferridurans

Depositor

Radhakrishnan Mahadevan

Publication

Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

Insert

Cm-sgRNA-dCas9

Use

CRISPR

Expression

Bacterial

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pTORKm43GWF

Plasmid

#112899

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mWasabi.

Depositor

Sebastian Schornack

Publication

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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p6xEGFP-N1

Plasmid

#122166

Purpose

Expression of 6xEGFP

Depositor

Georg Mayr

Publication

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

6xEGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pISceI-myl7:MBIC5-mCer3

Plasmid

#74117

Purpose

Zebrafish transgenic construct

Depositor

Michael Tsang

Publication

He et al Nat Methods. 2016 Mar;13(3):263-268. doi

Insert

MBIC5-mCer3

Use

Mrna generation for microinjection.

Tags

mCerulean 3

Expression

Mammalian

Promoter

SP6

Available since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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pDSAR-Cre-F2A-I-SceI

Plasmid

#62302

Purpose

Anopheles docking site transgenesis plasmid expressing Cre recombinase and I-SceI nuclease, vasa promoter, DsRed marker

Depositor

Eric Marois

Publication

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Insert

Cre recombinase, I-SceI nuclease

Expression

Insect

Promoter

vasa

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pT7mitfagRNA

Plasmid

#47931

Purpose

in vitro transcription of mitfa gRNA

Depositor

Wenbiao Chen

Publication

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 20;11

Insert

mitfa gRNA (mitfa Zebrafish)

Use

CRISPR

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-HM4Di-YFP

Plasmid

#209193

Purpose

Cre-dependent expression of Hm4Di fused to YFP; Chemogenetics

Depositor

Larry Zweifel

Publication

Sanford et al Neuron. 2017 Jan 4;93(1):164-178. doi: 1

Insert

Hm4Di

Use

AAV

Tags

YFP

Expression

Mammalian

Promoter

CAG

Available since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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CMVTO-GFP-ECL-3TM-tevs-KKMP V3

Plasmid

#179640

Purpose

TEVP-inducible membrane-bound GFP RELEASE construct

Depositor

Xiaojing Gao

Publication

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Membrane-bound GFP RELEASE

Expression

Mammalian

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-LMO-IRES-EGFP

Plasmid

#178309

Purpose

Express the bacterial lactate 2-monooxygenase (LMO) enzyme in mammalian cells

Depositor

Sergey Kasparov

Publication

Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

Insert

Lactate 2-monooxygenase

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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RNF146-c005

Plasmid

#173091

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Publication

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

RNF146 (RNF146 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

R99-L183, codon optimized

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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CAG.CenpB(FL)-GFP-Halo

Plasmid

#176205

Purpose

Centromeric anchor for TMP-Halo dimerization system

Depositor

Michael Lampson

Publication

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

CenpB-GFP-Halo

Expression

Mammalian

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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