We narrowed to 4,271 results for: pme

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pB-TopFlash-NanoLucP

Plasmid

#204347

Purpose

TopFlash reporter

Depositor

Li-Fang Chu

Article

Conrad et al Stem Cell Reports. 2023 Dec 12;18(12):23

Insert

TopFlash-NanoLucP

Use

Luciferase

Tags

NanoLucP

Promoter

miniP

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-hMC4R

Plasmid

#179315

Purpose

Express human melanocortin 4 receptor (GFP tagged) in mammalian cells

Depositor

Christian Gruber

Article

Muratspahic et al ACS Pharmacol Transl Sci. 2023 Sep 26;6(

Insert

melanocortin 4 receptor (MC4R Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pKL038 Lenti U6 dCas12a gRNA Puro mCherry

Plasmid

#195546

Purpose

dCas12a gRNA expression backbone

Depositor

Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti U6- empty cassette_Direct repeat_puro_mcherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ9714: pHR-PGK-SV40_NLS-dCasMINI-V4-NZF-c-Myc_NLS-mCherry-WPRE

Plasmid

#203220

Purpose

Expression of dCasMINI-V4-NZF-mCherry in mammalian cells

Depositor

Stanley Qi

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCasMINI-V4-NZF-mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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BLBP-mCherry

Plasmid

#63721

Purpose

Expressing mCherry from BLBP promoter which is a neural stem cell (radial glial cells) specific promoter

Depositor

Bart De Strooper

Article

Shariati et al J Cell Sci. 2013 Mar 1;126(Pt 5):1268-77

Insert

BLBP (Fabp7 Mouse)

Expression

Mammalian

Mutation

1700 bp upstream of BLBP gene cloned in CAG-mCher…

Promoter

1700bp upstream of BLBP gene

Available Since

April 1, 2015

Availability

Academic Institutions and Nonprofits only

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pRRL.SIN.EF1A.JAG1-B5.GS.CAR-3G

Plasmid

#194464

Purpose

CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.B5 in VH-VL order & (GGGGS)3 linker (scFv). GFP-Zeo for selection & monitoring.

Depositor

Ana Sofia Coroadinha

Article

Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

Insert

Anti-JAG1 CAR

Use

Lentiviral

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-ArchT-tdTomato

Plasmid

#28305

Purpose

Cre-dependent viral expression of ArchT-tdTomato for neuronal inhibition

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Has Service

AAV5

Insert

ArchT-tdTomato

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

tdTomato

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available Since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only

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Tol2-BetaActin-SecA5-YFP

Plasmid

#105664

Purpose

Ubiquitously expresses a secreted and fluorescently tagged Annexin5 for in-vivo apoptosis reporter

Depositor

Qing Deng

Article

Hsu et al Front Immunol. 2018 Mar 29;9:607. doi: 1

Insert

Secreted Annexin5-EYFP (ANXA5 Human)

Use

Tol2-gateway

Tags

EYFP

Promoter

Beta-Actin

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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BLBP-GFP

Plasmid

#63174

Purpose

Expressing GFP from BLBP promoter which is a neural stem cell (radial glial cells) specific promoter

Depositor

Bart De Strooper

Article

Shariati et al J Cell Sci. 2013 Mar 1;126(Pt 5):1268-77

Insert

BLBP (Fabp7 Mouse)

Expression

Mammalian

Promoter

1700bp upstream of BLBP gene

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pSLQ5465_pHR_hU6-crScaffold_EF1a-PuroR-T2A-BFP

Plasmid

#155307

Purpose

Rfx Cas13d guide cloning lentiviral vector with constitutively expressed puromycin resistance 2A-tagged with tagBFP

Depositor

Stanley Qi

Article

Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

Insert

Rfx Cas13d CRISPR RNA puromycin resistance 2A-tagged BFP

Use

Lentiviral

Expression

Mammalian

Promoter

hU6, EF1a

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGEN-Dre-nls (JDW 1131)

Plasmid

#229809

Purpose

A CAGGS-driven dre recombinase followed by a c-terminal 1x nls sequence

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

DRE recombinase-nls

Tags

SV40 NLS

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pOD2044-intDEG

Plasmid

#89366

Purpose

MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pelt-2 (intestine specific).

Depositor

Karen Oegema

Article

Wang et al Development. 2017 Jun 15. pii: dev.15009

Insert

vhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)

Use

Targeting vector for mos1 transposon mediated sin…

Tags

vhhGFP4 (GFP nanobody)

Promoter

Pelt-2

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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MigR1-AE

Plasmid

#12431

Depositor

Dong-Er Zhang

Article

Yan et al Proc Natl Acad Sci U S A. 2004 Dec 7. 10

Insert

AML1-ETO fusion (RUNX1, RUNX1T1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

June 2, 2010

Availability

Academic Institutions and Nonprofits only

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p3E-IRES-3xNLS-mScarlet-I3-pA (JDW 1360)

Plasmid

#224536

Purpose

A Gateway compatible 3' entry clone containing IRES-FLAG- NLS-mScarlet-I3

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

IRES-FLAG- NLS-mScarlet-I3

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-FbxO5-pcDNA3.1-

Plasmid

#52509

Purpose

expresses human FbxO5 in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

FbxO5 (FBXO5 Human, FbxO5)

Tags

FLAG-HA

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-GST-BcPC-PLC

Plasmid

#224255

Purpose

Express N-terminal HRV 3C protease cleavable GST fused to Bacillus cereus PC-PLC in E.coli

Depositor

Chiaki Murakami

Article

Murakami et al Anal Biochem. 2017 Jun 1;526:43-49. doi:

Insert

Bacillus cereus phospholipase C (plC, plC Bacillus cereus ATCC 14579)

Tags

GST and HRV-3C (LEVLFQGP) site

Expression

Bacterial

Mutation

The gene of Bacillus cereus ATCC 14579 was used f…

Promoter

lac promoter

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV5-hBMPR-1A CA

Plasmid

#49527

Purpose

expresses constitutively active human BMP receptor 1A

Depositor

Lee Niswander, Peter ten Dijke

Article

Varley et al Dev Biol. 1998 Apr 1;196(1):107-18.

Insert

BMPR-1A CA (BMPR1A Human)

Expression

Mammalian

Mutation

constitutively active mutation (Q233D); also P2A …

Promoter

CMV

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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pCAGEN-rtTA-IRES-nls-EGFP-WPRE-bGH-pA (JDW 410)

Plasmid

#229807

Purpose

A CAGGS driven tet-on transactivator with an nls-EGFP reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

rtTA-IRES-nls-EGFP

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-AuroraB kd K106R

Plasmid

#108493

Purpose

expression of EGFP-AuroraB kinase dead (K106R)

Depositor

Susanne Lens

Article

Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

Insert

AuroraB (AURKB Human)

Tags

EGFP

Expression

Mammalian

Mutation

Kinase death K106A

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-WPRE-SV40-pA (JDW 922)

Plasmid

#224545

Purpose

A Gateway compatible 3' entry clone containing a woodchuck herpes simplex virus regulatory element (WPRE) to stabilize mRNA upstream of an SV40 polyA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

WPRE-SV40-pA

Use

Gateway cloning

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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