Addgene

We narrowed to 28,391 results for: REP

Showing: 5461 - 5490 of 28391 results

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lenti-SpCas9 neo

Plasmid

#104996

Purpose

This lentiviral construct delivers hSpCas9 and G418 resistance.

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

KpnI-XhoI-BsrGI-NheI

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2 blast

Plasmid

#98293

Purpose

Variant of lentiCRISPRv2 that confers blasticidin S resistance

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

EF-1a

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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lenti-SpCas9 hygro

Plasmid

#104995

Purpose

This lentiviral construct delivers hSpCas9 and hygromycin resistance.

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

KpnI-XhoI-BsrGI-NheI

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-Alb-mScarlet

Plasmid

#183461

Purpose

To produce AAV with the P3 promoter to express Alb(mouse)-mScarlet fusion protein

Depositor

Hajime Hirase

Article

Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

Insert

Alb (Alb Mouse)

Use

AAV

Tags

mScarlet

Expression

Mutation

Promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2 hygro

Plasmid

#98291

Purpose

Variant of lentiCRISPRv2 that confers hygromycin resistance

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

EF-1a

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2 neo

Plasmid

#98292

Purpose

Variant of lentiCRISPRv2 that confers G418 resistance

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

EF-1a

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2 puro

Plasmid

#98290

Purpose

Variant of lentiCRISPRv2 that confers puromycin resistance

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

EF-1a

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CBh-Alb-mNG

Plasmid

#183466

Purpose

To produce AAV with the CBh promoter to express Alb(mouse)-mNeonGreen fusion protein

Depositor

Hajime Hirase

Article

Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

Insert

Alb (Alb Mouse)

Use

AAV

Tags

mNeonGreen

Expression

Mutation

Promoter

Available since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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3X FLAG-FUS-WT

Plasmid

#44985

Depositor

Robin Reed

Article

Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

Insert

FUS (Full length) (FUS Human)

Use

Tags

3X FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

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pPGC1a_Proximal Promoter-FLuc2P

Plasmid

#154259

Purpose

Destabilized firefly luciferase reporter containing the Human PGC-1alpha Proximal Promoter from -500 to +52.

Depositor

Antonio Amelio

Article

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

PGC-1a Proximal Promoter (PPARGC1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Human PGC-1alpha Proximal Promoter

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pPGC1a_Distal Promoter-FLuc2P

Plasmid

#154260

Purpose

Destabilized firefly luciferase reporter containing the upstream Human PGC-1alpha Distal Promoter from -500 to +52.

Depositor

Antonio Amelio

Article

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

PGC-1a Distal Promoter (PPARGC1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Human PGC-1alpha Distal Promoter

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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PB-TRE-OKSM

Plasmid

#204498

Purpose

Expresses Oct4, Klf4, Sox2 and c-Myc by tet-on promoter in mammalian cells

Depositor

Wataru Fujii

Article

Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

Inserts

OCT4 (POU5F1 Human)
KLF4 (KLF4 Human)
SOX2 (SOX2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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VGAT scFv [L118/14]

Plasmid

#206754

Purpose

Mammalian Expression of VGAT scFV. Derived from hybridoma L118/14 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

VGAT (Mus musculus) recombinant scFV (Slc32a1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-BrCas12b-RFND

Plasmid

#195339

Purpose

Expresses Engineered BrCas12b in Bacteria

Depositor

Piyush Jain

Article

Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

Insert

BrCas12b

Use

CRISPR and Synthetic Biology

Tags

6xHis and 6xHis, MBP, TEV cleavage site

Expression

Bacterial

Mutation

R160E, F208W, N524V, D868V

Promoter

T7

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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lenti-SpCas9 puro

Plasmid

#104994

Purpose

This 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

KpnI-XhoI-BsrGI-NheI

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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Gephyrin scFv [L106/23]

Plasmid

#206775

Purpose

Mammalian Expression of Gephyrin scFV. Derived from hybridoma L106/23 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Gephyrin (Homo sapiens) recombinant scFV (GPHN Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-Alb-mNG

Plasmid

#183460

Purpose

To produce AAV with the P3 promoter to express Alb(mouse)-mNeonGreen fusion protein

Depositor

Hajime Hirase

Article

Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

Insert

Alb (Alb Mouse)

Use

AAV

Tags

mNeonGreen

Expression

Mutation

Promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-EGFP-FLAG-cGAS

Plasmid

#127661

Purpose

Over-express EGFP-FLAG-cGAS

Depositor

Nicolas Manel

Article

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

cGAS (CGAS Human)

Use

Lentiviral

Tags

EGFP-Flag

Expression

Mutation

Promoter

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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CAG-Cas9-T2A-EGFP-ires-puro

Plasmid

#78311

Purpose

Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.

Depositor

Timo Otonkoski

Article

Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi

Insert

WT SpCas9-T2A-EGFP-ires-puromycin resistance

Use

CRISPR

Tags

T2A-EGFP-ires-puro

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-EGFP-NLS-FLAG-cGAS

Plasmid

#127659

Purpose

Over-express EGFP-NLS-FLAG-cGAS

Depositor

Nicolas Manel

Article

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

cGAS (CGAS Human)

Use

Lentiviral

Tags

EGFP-NLS-Flag

Expression

Mutation

Promoter

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)

Plasmid

#71707

Purpose

A human codon-optimized SpCas9 fused to first 110 amino acids of human GEMININ and chimeric guide RNA expression plasmid

Depositor

Giulio Draetta

Article

Gutschner et al Cell Rep. 2016 Feb 3. pii: S2211-1247(16

Insert

humanized S. pyogenes Cas9 fused to human Geminin (GMNN Human)

Use

CRISPR

Tags

3xFLAG and hGEM(1/110)

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-EGFP-NLS-FLAG- cGAS E225A/D227A

Plasmid

#127660

Purpose

Over-express catalytically inactive EGFP-NLS-FLAG-cGAS E225A/D227A

Depositor

Nicolas Manel

Article

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

cGAS (CGAS Human)

Use

Lentiviral

Tags

Expression

Mutation

E225A/D227A

Promoter

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-SPHKAP [L131/17R]

Plasmid

#177477

Purpose

Mammalian Expression Plasmid of anti-SPHKAP (Mouse). Derived from hybridoma L131/17.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-SPHKAP (Mus musculus) recombinant mouse monoclonal antibody (Sphkap Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-EGFP-FLAG-cGAS E225A/D227A

Plasmid

#127662

Purpose

Over-express catalytically inactive EGFP-FLAG-cGAS E225A/D227A

Depositor

Nicolas Manel

Article

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

cGAS (CGAS Human)

Use

Lentiviral

Tags

Expression

Mutation

E225A/D227A

Promoter

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pNL-CEF-CD79B-YFP

Plasmid

#187006

Purpose

Expression of human CD79B as YFP fusion protein

Depositor

Hermann Eibel

Article

Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

Insert

CD79B (CD79B Human)

Use

Lentiviral

Tags

YFP

Expression

Mutation

Promoter

Available since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-Neurofascin (extracellular) [A12/18R]

Plasmid

#177438

Purpose

Mammalian Expression Plasmid of anti-Pan-Neurofascin (extracellular) (Rat). Derived from hybridoma A12/18.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Pan-Neurofascin (extracellular) (Rattus norvegicus) recombinant mouse monoclonal antibody (Nfasc Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Homer1L scFv [L113/13]

Plasmid

#190498

Purpose

Mammalian Expression of Homer1L scFV. Derived from hybridoma L113/13.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Homer1L (Mus musculus) recombinant scFV (Homer1 Mouse)

Use

Tags

Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3 ss-3xFLAG-hLRP5 (aa 32-1615)

Plasmid

#115788

Purpose

Expresses human LRP5 with N-terminal 3xflag tag in mammalian cells, contains artificial signal sequence upstream of tag.

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

LRP5 (LRP5 Human)

Use

Tags

3xflag

Expression

Mammalian

Mutation

contains silent mutation that creates an internal…

Promoter

CMV

Available since

Oct. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Blast-DNTGFBR2-HA

Plasmid

#130888

Purpose

Expresses dominant negative mutant human TGFBR2

Depositor

Gian-Paolo Dotto

Article

Bordignon et al Cell Rep. 2019 Aug 27;28(9):2358-2372.e6

Insert

TGFBR2 (TGFBR2 Human)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

Dominant Negative receptor lacking cytosolic cata…

Promoter

CMV

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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PSD-95 scFv [K28/43]

Plasmid

#182066

Purpose

Mammalian Expression of PSD-95 scFv. Derived from hybridoma K28/43.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting PSD-95 (Homo sapiens) (DLG4 Mouse)

Use

Tags

HA, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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