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pCS2-Prom1-TEVp-TwinStrep-His / IRES2 / Pcdh21-TEVp-Myc-Flag

Plasmid

#210820

Purpose

Mammalian overexpression vector for polycistronic co-expression of C-terminally Strep and His tagged human Prom1s1 (WT) and C-terminally Myc and Flag tagged human Pchd21 (WT)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Inserts

Prominin 1 (PROM1 Human)
Protocadherin 21 (CDHR1 Human)

Tags

Myc, 3xFlag and TwinStrep, 10xHis

Expression

Mammalian

Promoter

CMV and CMV / IRES2

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMH003_phiC31_neo-5xTetO-pEF-H2B-mCitrine-coreHS4-pRSV-H2B-mCherry

Plasmid

#179431

Purpose

Dual fluorescent reporter construct with single core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

TRE-cit-coreHS4-mch (SC)

Expression

Mammalian

Promoter

pEF alpha, pRSV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-KLF6

Plasmid

#192909

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of KLF6

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

KLF6 (KLF6 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-KLF4-P2A-mCherry

Plasmid

#203855

Purpose

Tet-inducible lentiviral plasmid expressing KLF4-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

KLF4 (KLF4 Human)

Expression

Mammalian

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-LRRK2-G2019S-3a

Plasmid

#180432

Purpose

Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

Depositor

Frank Soldner

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

ncRNA targeting LRRK2-G2019S (LRRK2 Human)

Use

CRISPR

Expression

Mammalian

Mutation

LRRK2-G2019S

Promoter

U6

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-SynCAM4 [N244/5R]

Plasmid

#114562

Purpose

Mammalian Expression Plasmid of anti-SynCAM4 (Mouse). Derived from hybridoma N244/5.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-SynCAM4 (Mus musculus) recombinant mouse monoclonal antibody (Cadm4 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Kv1.4 K+ channel [K13/31R]

Plasmid

#128613

Purpose

Mammalian Expression Plasmid of anti-Kv1.4 K+ channel (Rat). Derived from hybridoma K13/31.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Kv1.4 K+ Channel (Rat) recombinant mouse monoclonal antibody (Kcna4 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-ID2-P2A-mCherry

Plasmid

#203865

Purpose

Tet-inducible lentiviral plasmid expressing ID2-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

ID2 (ID2 Human)

Expression

Mammalian

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX307-mFzd4

Plasmid

#102866

Purpose

Expresses mouse Fzd4 in mammalian cells

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Frizzled 4 (Fzd4 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

T to C substitution at nucleotide 1149 of Fzd4 OR…

Promoter

EF1A

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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Anti-GIT2 [N83/48R]

Plasmid

#140079

Purpose

Mammalian Expression Plasmid of anti-GIT2 (Human). Derived from hybridoma N83/48.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-GIT2 (Human) recombinant mouse monoclonal antibody (GIT2 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX307-mFzd8

Plasmid

#102870

Purpose

Expresses mouse Fzd8 in mammalian cells

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Frizzled 8 (Fzd8 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

T to C substitution at nucleotide 1134, C to T su…

Promoter

EF1A

Available Since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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Anti-Cav3.1 Ca2+ channel [N178A/9.1R]

Plasmid

#114520

Purpose

Mammalian Expression Plasmid of anti-Cav3.1 Ca2+ channel (Mouse). Derived from hybridoma N178A/9.1.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Cav3.1 Ca2+ channel (Mus musculus) recombinant mouse monoclonal antibody (Cacna1g Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pIRES2-CMV-nAChRb2(E61C)-IRES-GFP

Plasmid

#164779

Purpose

cysteine mutated nAChR beta2 subunit (E61C) for the attachment of a photoswitchable tethered ligand

Depositor

Alexandre Mourot

Article

Durand-de Cuttoli et al Elife. 2018 Sep 4;7. pii: 37487. doi: 10

Insert

nAChR b2 E61C - IRES- GFP (Chrnb2 Mouse)

Expression

Mammalian

Mutation

E61C

Promoter

CMV

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hWWTR1-W2B)-PGKpuro2AmCherry-W

Plasmid

#163177

Purpose

Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of mCherry tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

WWTR1 (WWTR1 Human)

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

U6

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-SUR1 [N289/16R]

Plasmid

#114556

Purpose

Mammalian Expression Plasmid of anti-SUR1 (Rat). Derived from hybridoma N289/16.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-SUR1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Abcc8 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pLEX307-mFzd9

Plasmid

#102877

Purpose

Expresses mouse Fzd9 in mammalian cells

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Frizzled 9 (Fzd9 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

A to G substitution at nucleotide 114, G to A sub…

Promoter

EF1A

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-Nav1 Na+ channel [N419/78R]

Plasmid

#114553

Purpose

Mammalian Expression Plasmid of anti-Pan-Nav1 Na+ channel (Human). Derived from hybridoma N419/78.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Pan-Nav1 Na+ channel (Homo sapiens) recombinant mouse monoclonal antibody (SCN5A Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-NR3C1-P2A-mCherry

Plasmid

#203854

Purpose

Tet-inducible lentiviral plasmid expressing NR3C1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

NR3C1 (NR3C1 Human)

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 Luciferase

Plasmid

#117072

Purpose

single guide RNA targeting Luciferase

Depositor

William Hahn

Article

Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

Insert

Luciferase

Use

CRISPR

Promoter

hU6

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Cavbeta1 Ca2+ channel [N7/18.1R]

Plasmid

#114497

Purpose

Mammalian Expression Plasmid of anti-Cavbeta1 Ca2+ channel (Human). Derived from hybridoma N7/18.1.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Cavbeta1 Ca2+ channel (Home sapiens) recombinant mouse monoclonal antibody (CACNB1 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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